Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P06_F_J23
         (694 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit...    78   1e-15
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po...    64   2e-11
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta...    33   0.030
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces...    28   1.1  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    27   2.6  
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ...    27   2.6  
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ...    26   4.5  
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo...    26   4.5  
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c...    26   5.9  

>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 77.8 bits (183), Expect = 1e-15
 Identities = 51/175 (29%), Positives = 88/175 (50%), Gaps = 4/175 (2%)
 Frame = +2

Query: 149 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 316
           N   D   KFP  K+P F   DG   L+E+ AIA+Y+A+     +L G     +A+V Q+
Sbjct: 38  NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96

Query: 317 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 496
            S+++SEL  A    + P +    +++Q  + A++ +       D  L ++T+LV  R+T
Sbjct: 97  CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156

Query: 497 LADVIVFSTLLHAFQHVLXPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAP 661
           LAD+     L     +VL  S  +   ++ R++ T+ HQ ++ A+   L     P
Sbjct: 157 LADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAITEPLKFIDQP 211


>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 220

 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 50/206 (24%), Positives = 94/206 (45%), Gaps = 8/206 (3%)
 Frame = +2

Query: 29  MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 208
           M  G LY++  N R    L  A+     V +   +       S D   KFP  K+P F  
Sbjct: 1   MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57

Query: 209 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 364
           ADG  L      + Y+         E L   +   +A + +W  + + +++ P +   WV
Sbjct: 58  ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117

Query: 365 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQH 544
             + G + + ++  + + +  + +LK+ +  +  RT+LV +R TLAD+   S L   F  
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFFNS 177

Query: 545 VLXPSVRSSLINVQRWFLTVAHQPQV 622
           ++    R  L ++ R+++T+ HQ ++
Sbjct: 178 IIDEKTRKELPHLTRYYITMFHQAKL 203


>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 450

 Score = 33.5 bits (73), Expect = 0.030
 Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
 Frame = +2

Query: 446 LDGHLLTRTFLVTER-ITLADVIVFSTLLHAFQHVLXPSVRSSLINVQRWFLTVAHQPQV 622
           LD  L + TF+  +  I++AD+ V++ + H++   L       L NV RWF  + HQ  V
Sbjct: 128 LDEFLKSSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESV 186

Query: 623 SAVVGSLTL 649
                S+++
Sbjct: 187 MEAANSMSM 195


>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = +3

Query: 423 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 515
           TY PP  Y  D+FS APS+   +     S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 16/62 (25%), Positives = 30/62 (48%)
 Frame = +2

Query: 56  PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 235
           P  F++ KA +  QY+          +FG  +K ++F K+    ++  F S +  +L+  
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611

Query: 236 SN 241
           SN
Sbjct: 612 SN 613


>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 267

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
 Frame = +3

Query: 285 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 380
           +W+P PV  SG    T   C+ LP  G  +T V
Sbjct: 23  LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55


>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 369

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -2

Query: 225 STFPSALSNAGTFPAGNFFKKSSDL 151
           +TFP A +  GTFP G  F   S L
Sbjct: 52  ATFPWAQAPVGTFPIGQLFDPVSGL 76


>SPBC16A3.11 |eso1||sister chromatid cohesion protein
           Eso1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = +2

Query: 236 SNAIAYYVANESLRGGDLATQARVWQWAS 322
           S + A Y+ NE+LR G +   +R+W  AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808


>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 339

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
 Frame = +3

Query: 315 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 422
           GH  L  + C  P++GSS  L+  +C   N R+  V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,996,690
Number of Sequences: 5004
Number of extensions: 63874
Number of successful extensions: 209
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)