Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P06_F_B15
(642 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 55 8e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 2e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 3e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.007
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.3
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 5.3
SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|... 26 5.3
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 25 7.0
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 25 7.0
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 55.2 bits (127), Expect = 8e-09
Identities = 29/78 (37%), Positives = 43/78 (55%)
Frame = +3
Query: 111 TAKQRKTKTRGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCM 290
T RK K E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA- 130
Query: 291 DPEDDDGMIPYAAFLKKV 344
D DG+I Y F + +
Sbjct: 131 -DTDGDGVINYEEFSRVI 147
Score = 29.9 bits (64), Expect = 0.33
Identities = 18/68 (26%), Positives = 36/68 (52%)
Frame = +3
Query: 153 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 332
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 333 LKKVMAXK 356
L +MA K
Sbjct: 71 L-TMMARK 77
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 44.0 bits (99), Expect = 2e-05
Identities = 23/70 (32%), Positives = 42/70 (60%)
Frame = +3
Query: 141 GAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIP 320
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+
Sbjct: 74 GDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVN 130
Query: 321 YAAFLKKVMA 350
Y F++ ++A
Sbjct: 131 YHDFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 43.2 bits (97), Expect = 3e-05
Identities = 26/84 (30%), Positives = 50/84 (59%)
Frame = +3
Query: 99 SFPSTAKQRKTKTRGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVT 278
SF S K R+T++ E++++ +++DK+ +G + A+ + LGEKL D+EV +
Sbjct: 65 SFVSN-KLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMV 120
Query: 279 KDCMDPEDDDGMIPYAAFLKKVMA 350
++ DP + G Y F++++MA
Sbjct: 121 QEA-DP-TNSGSFDYYDFVQRIMA 142
Score = 30.7 bits (66), Expect = 0.19
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +3
Query: 150 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 329
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.007
Identities = 21/74 (28%), Positives = 37/74 (50%)
Frame = +3
Query: 63 RRRARSCSHSKSSFPSTAKQRKTKTRGAYEDFLECLKLYDKNENGLMLGAELTHTLLALG 242
+RR+R+ S + + A R T +D E KL+D +++ + EL + ALG
Sbjct: 8 KRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
Query: 243 EKLDDSEVAEVTKD 284
+ SEV ++ +D
Sbjct: 68 FNAEKSEVLKILRD 81
Score = 27.9 bits (59), Expect = 1.3
Identities = 19/74 (25%), Positives = 31/74 (41%)
Frame = +3
Query: 126 KTKTRGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 305
K R E+ +L+D +E G + L L E +DD E+ + ++ D
Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLDQ 159
Query: 306 DGMIPYAAFLKKVM 347
DG I F+ +M
Sbjct: 160 DGEINEQEFIAIMM 173
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.3
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -2
Query: 503 APPEELSPPRALPAPVPQSRASVF*GPSHRT 411
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 5.3
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 237 LGEKLDDSEVAEVTKDCMDPED 302
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 182
Score = 25.8 bits (54), Expect = 5.3
Identities = 12/29 (41%), Positives = 13/29 (44%)
Frame = -1
Query: 615 ESCTKQLILCCSYKKGVCPYXRCYTGCRR 529
ESC K+ CC KK C GC R
Sbjct: 78 ESCEKKKPKCCEKKKPKCCESEQNNGCGR 106
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 25.4 bits (53), Expect = 7.0
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +3
Query: 63 RRRARSCSHSKSSFPSTAKQRKTKTRGAYE-DFLECLKLYDKNENGLM 203
RR+ S SH +SF + + +K R ++ D L+ L + + E+G++
Sbjct: 36 RRKHASHSHDDASFFDPSNEEASKLRESFAMDTLDALLQWFEEEDGVV 83
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 25.4 bits (53), Expect = 7.0
Identities = 15/47 (31%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
Frame = +3
Query: 51 SVVQRRRARS-CSHSKSSFPSTAKQRKTKTRGAYEDFLECLKLYDKN 188
S + +RARS C H SS ST G C++ Y+K+
Sbjct: 178 SAIAGKRARSLCFHVLSSKSSTFYSLTESKSGLVNSVDRCIQFYEKS 224
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,243,324
Number of Sequences: 5004
Number of extensions: 39326
Number of successful extensions: 121
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -