Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P05_pT_P13
(748 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 325 4e-90
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 325 4e-90
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 325 4e-90
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 100 4e-22
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 60 4e-10
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 57 3e-09
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.8
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 6.6
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.7
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 325 bits (799), Expect = 4e-90
Identities = 149/193 (77%), Positives = 168/193 (87%)
Frame = -3
Query: 746 PLQDVYXIGGIGTVPVGRVETGVLKXGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNV 567
PLQDVY IGGIGTVPVGRVETGV+K G IV FAPA + TEVKSVEMHHE+L +PGDNV
Sbjct: 247 PLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNV 306
Query: 566 GFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHI 387
GFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHI
Sbjct: 307 GFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHI 366
Query: 386 ACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRD 207
ACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRD
Sbjct: 367 ACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRD 426
Query: 206 MRQTVAVGVIKAV 168
MRQTVAVGVIKAV
Sbjct: 427 MRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 325 bits (799), Expect = 4e-90
Identities = 149/193 (77%), Positives = 168/193 (87%)
Frame = -3
Query: 746 PLQDVYXIGGIGTVPVGRVETGVLKXGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNV 567
PLQDVY IGGIGTVPVGRVETGV+K G IV FAPA + TEVKSVEMHHE+L +PGDNV
Sbjct: 247 PLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNV 306
Query: 566 GFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHI 387
GFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHI
Sbjct: 307 GFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHI 366
Query: 386 ACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRD 207
ACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRD
Sbjct: 367 ACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRD 426
Query: 206 MRQTVAVGVIKAV 168
MRQTVAVGVIKAV
Sbjct: 427 MRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 325 bits (799), Expect = 4e-90
Identities = 149/193 (77%), Positives = 168/193 (87%)
Frame = -3
Query: 746 PLQDVYXIGGIGTVPVGRVETGVLKXGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNV 567
PLQDVY IGGIGTVPVGRVETGV+K G IV FAPA + TEVKSVEMHHE+L +PGDNV
Sbjct: 247 PLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNV 306
Query: 566 GFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHI 387
GFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHI
Sbjct: 307 GFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHI 366
Query: 386 ACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRD 207
ACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRD
Sbjct: 367 ACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRD 426
Query: 206 MRQTVAVGVIKAV 168
MRQTVAVGVIKAV
Sbjct: 427 MRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 99.5 bits (237), Expect = 4e-22
Identities = 58/186 (31%), Positives = 99/186 (53%), Gaps = 2/186 (1%)
Frame = -3
Query: 716 IGTVPVGRVETGVLKXGTIVVFAPANIXTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSV 540
+GT+ G++E G +K + V+ P N EV ++ + E + ++ GD V V+
Sbjct: 480 LGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD- 538
Query: 539 KELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKE 360
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++
Sbjct: 539 SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLH 597
Query: 359 KVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG- 183
K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG
Sbjct: 598 KLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGK 656
Query: 182 VIKAVN 165
V+K ++
Sbjct: 657 VVKILD 662
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 59.7 bits (138), Expect = 4e-10
Identities = 49/191 (25%), Positives = 85/191 (44%), Gaps = 2/191 (1%)
Frame = -3
Query: 743 LQDVYXIGGIGTVPVGRVETGVLKXGTIV--VFAPANIXTEVKSVEMHHEALQEAVPGDN 570
++DV+ I G GTV GRVE G LK G + V +++ T V +EM + L AV GDN
Sbjct: 257 IEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDN 316
Query: 569 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 390
G ++++ ++L+RG + P F A +L + T +D +
Sbjct: 317 CGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQ 372
Query: 389 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 210
+ + +++ ++ T + K + GD + P+ +E Q RF VR
Sbjct: 373 LYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVR 423
Query: 209 DMRQTVAVGVI 177
+ TV ++
Sbjct: 424 EGGSTVGTALV 434
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 56.8 bits (131), Expect = 3e-09
Identities = 54/191 (28%), Positives = 85/191 (44%), Gaps = 1/191 (0%)
Frame = -3
Query: 743 LQDVYXIGGIGTVPVGRVETGVLKXGTIVVFAPANIXTEVKSVEMHHEALQE-AVPGDNV 567
+ DVY TV GRVE G ++ ++ + VK+V + + AV GD V
Sbjct: 409 IDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTV 467
Query: 566 GFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHI 387
+ ++ V +LR G + + +N P + F A++ + G I +G T VL H+
Sbjct: 468 TLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HL 521
Query: 386 ACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRD 207
+ K+ K + + S K I L PLC+ +E P LGRF +R
Sbjct: 522 GRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRR 578
Query: 206 MRQTVAVGVIK 174
TVA G++K
Sbjct: 579 SGDTVAAGIVK 589
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +3
Query: 531 QFLDGHVLYVETYIVSRYSFLESFV 605
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 6.6
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +1
Query: 124 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 297
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 298 SPDLMDFGLTSVDLPVRRSTFSLIS 372
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +1
Query: 289 MAASPDLMDFGLTSVDLPVRRSTFS 363
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,065,733
Number of Sequences: 5004
Number of extensions: 61531
Number of successful extensions: 181
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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