Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P05_pT_M22
         (721 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    57   3e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    45   1e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    43   4e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    29   0.51 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   1.5  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   6.2  
SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|ch...    26   6.2  
SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|...    25   8.2  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 56.8 bits (131), Expect = 3e-09
 Identities = 29/75 (38%), Positives = 44/75 (58%)
 Frame = -1

Query: 625 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 446
           A+K KD    E+  E  K++DK+ NG +   ELTH L +LGE+L   EVA++ ++     
Sbjct: 75  ARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132

Query: 445 DDDGMIPYAAFLKKV 401
           D DG+I Y  F + +
Sbjct: 133 DGDGVINYEEFSRVI 147



 Score = 29.1 bits (62), Expect = 0.67
 Identities = 15/61 (24%), Positives = 32/61 (52%)
 Frame = -1

Query: 592 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 413
           +F E   L+D++++G +   EL   + +LG+    +E+ ++  +     D +G I +  F
Sbjct: 13  EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70

Query: 412 L 410
           L
Sbjct: 71  L 71


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 25/84 (29%), Positives = 47/84 (55%)
 Frame = -1

Query: 646 FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVT 467
           FL + ++       G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ 
Sbjct: 60  FLQVLNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119

Query: 466 KDCMDPEDDDGMIPYAAFLKKVMA 395
           K        DGM+ Y  F++ ++A
Sbjct: 120 KGV---PVKDGMVNYHDFVQMILA 140


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 21/77 (27%), Positives = 46/77 (59%)
 Frame = -1

Query: 625 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 446
           + K ++  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  + ++  DP 
Sbjct: 68  SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP- 125

Query: 445 DDDGMIPYAAFLKKVMA 395
            + G   Y  F++++MA
Sbjct: 126 TNSGSFDYYDFVQRIMA 142



 Score = 30.7 bits (66), Expect = 0.22
 Identities = 14/60 (23%), Positives = 34/60 (56%)
 Frame = -1

Query: 595 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 416
           ++  E   LYD +++GL+  + +   L +LG  + D+E+A+++ +  D  D+   + + +
Sbjct: 9   DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 29.5 bits (63), Expect = 0.51
 Identities = 15/58 (25%), Positives = 30/58 (51%)
 Frame = -1

Query: 634 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 461
           Y+  + +  +   +D  E  KL+D +++  +   EL   + ALG   + SEV ++ +D
Sbjct: 24  YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81



 Score = 27.1 bits (57), Expect = 2.7
 Identities = 18/75 (24%), Positives = 33/75 (44%)
 Frame = -1

Query: 622 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 443
           +K  ++   E+     +L+D +E G +    L      L E +DD E+  + ++     D
Sbjct: 101 EKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLD 158

Query: 442 DDGMIPYAAFLKKVM 398
            DG I    F+  +M
Sbjct: 159 QDGEINEQEFIAIMM 173


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 13/31 (41%), Positives = 15/31 (48%)
 Frame = +3

Query: 246  APPEELSPPRALPAPVPQSRASVF*GPSHRT 338
            APP    PP A P P+P S A     P  R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = -1

Query: 508 LGEKLDDSEVAEVTKDCMDPED 443
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


>SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 204

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 16/49 (32%), Positives = 22/49 (44%), Gaps = 5/49 (10%)
 Frame = -2

Query: 201 HLRYG--QTPFLYEQHNINCFVQLSTFDWTDERKMKETGKHTL---CVF 70
           HL  G  Q  F   + N+    + S+F W D  K+KE     L   C+F
Sbjct: 43  HLEKGLLQQCFNLVKKNMEALYRQSSFGWDDSEKLKEMEMEKLEYICIF 91


>SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 182

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 12/29 (41%), Positives = 13/29 (44%)
 Frame = +2

Query: 134 ESCTKQLILCCSYKKGVCPYLRCYTGCRR 220
           ESC K+   CC  KK  C       GC R
Sbjct: 78  ESCEKKKPKCCEKKKPKCCESEQNNGCGR 106


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,599,064
Number of Sequences: 5004
Number of extensions: 48419
Number of successful extensions: 148
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)