Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P05_pT_J15
(786 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 316 3e-87
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 316 3e-87
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 316 3e-87
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 100 5e-22
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 2e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 53 4e-08
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 7.0
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 316 bits (775), Expect = 3e-87
Identities = 144/186 (77%), Positives = 163/186 (87%)
Frame = -3
Query: 784 IGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNVGFNVKNV 605
IGGIGTVPVGRVETGV+KPG IV FAPA + TEVKSVEMHHE+L +PGDNVGFNVKNV
Sbjct: 254 IGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNV 313
Query: 604 SVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEI 425
SVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+
Sbjct: 314 SVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAEL 373
Query: 424 KEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAV 245
EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAV
Sbjct: 374 IEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAV 433
Query: 244 GVIKAV 227
GVIKAV
Sbjct: 434 GVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 316 bits (775), Expect = 3e-87
Identities = 144/186 (77%), Positives = 163/186 (87%)
Frame = -3
Query: 784 IGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNVGFNVKNV 605
IGGIGTVPVGRVETGV+KPG IV FAPA + TEVKSVEMHHE+L +PGDNVGFNVKNV
Sbjct: 254 IGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNV 313
Query: 604 SVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEI 425
SVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+
Sbjct: 314 SVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAEL 373
Query: 424 KEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAV 245
EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAV
Sbjct: 374 IEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAV 433
Query: 244 GVIKAV 227
GVIKAV
Sbjct: 434 GVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 316 bits (775), Expect = 3e-87
Identities = 144/186 (77%), Positives = 163/186 (87%)
Frame = -3
Query: 784 IGGIGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEALQEAVPGDNVGFNVKNV 605
IGGIGTVPVGRVETGV+KPG IV FAPA + TEVKSVEMHHE+L +PGDNVGFNVKNV
Sbjct: 254 IGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNV 313
Query: 604 SVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEI 425
SVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+
Sbjct: 314 SVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAEL 373
Query: 424 KEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAV 245
EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAV
Sbjct: 374 IEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAV 433
Query: 244 GVIKAV 227
GVIKAV
Sbjct: 434 GVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 99.5 bits (237), Expect = 5e-22
Identities = 58/186 (31%), Positives = 99/186 (53%), Gaps = 2/186 (1%)
Frame = -3
Query: 775 IGTVPVGRVETGVLKPGTIVVFAPANIXTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSV 599
+GT+ G++E G +K + V+ P N EV ++ + E + ++ GD V V+
Sbjct: 480 LGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD- 538
Query: 598 KELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKE 419
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++
Sbjct: 539 SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLH 597
Query: 418 KVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG- 242
K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG
Sbjct: 598 KLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGK 656
Query: 241 VIKAVN 224
V+K ++
Sbjct: 657 VVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 54.4 bits (125), Expect = 2e-08
Identities = 49/176 (27%), Positives = 79/176 (44%), Gaps = 1/176 (0%)
Frame = -3
Query: 757 GRVETGVLKPGTIVVFAPANIXTEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRG 581
GRVE G ++ ++ + VK+V + + AV GD V + ++ V +LR G
Sbjct: 423 GRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPG 482
Query: 580 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 401
+ + +N P + F A++ + G I +G T VL H+ + K+
Sbjct: 483 DILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVN 536
Query: 400 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 233
K + + S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 537 NKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 53.2 bits (122), Expect = 4e-08
Identities = 47/185 (25%), Positives = 80/185 (43%), Gaps = 2/185 (1%)
Frame = -3
Query: 784 IGGIGTVPVGRVETGVLKPGTIV--VFAPANIXTEVKSVEMHHEALQEAVPGDNVGFNVK 611
I G GTV GRVE G LK G + V +++ T V +EM + L AV GDN G ++
Sbjct: 263 ISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLR 322
Query: 610 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 431
++ ++L+RG + P F A +L + T +D + + + +
Sbjct: 323 SIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQLYSRTS 378
Query: 430 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 251
++ ++ T + K + GD + P+ +E Q RF VR+ TV
Sbjct: 379 DVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVREGGSTV 429
Query: 250 AVGVI 236
++
Sbjct: 430 GTALV 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 590 QFLDGHVLYVETYIVSRYSFLESFV 664
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.0
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +3
Query: 183 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 356
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 357 SPDLMDFGLTSVDLPVRRSTFSLIS 431
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.3
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +3
Query: 348 MAASPDLMDFGLTSVDLPVRRSTFS 422
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,167,348
Number of Sequences: 5004
Number of extensions: 64286
Number of successful extensions: 192
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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