Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P05_pT_G03
         (704 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po...   204   1e-53
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po...   189   4e-49
SPAC23H3.05c |swd1||COMPASS complex subunit Swd1|Schizosaccharom...    27   2.0  
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb...    27   2.6  
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce...    26   4.6  
SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   8.0  
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S...    25   8.0  

>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  204 bits (498), Expect = 1e-53
 Identities = 95/130 (73%), Positives = 110/130 (84%)
 Frame = -2

Query: 703 HLIPKXSNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPT 524
           HLIP  ++ ESKVFY KMKGDYYRYLAE A GE R    + S + Y+ A EI+ A++ PT
Sbjct: 109 HLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPT 168

Query: 523 HPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRD 344
           HPIRLGLALNFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L+E+SYKDSTLIMQLLRD
Sbjct: 169 HPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRD 228

Query: 343 NLTLWTSDTQ 314
           NLTLWTSD +
Sbjct: 229 NLTLWTSDAE 238


>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  189 bits (460), Expect = 4e-49
 Identities = 88/130 (67%), Positives = 105/130 (80%)
 Frame = -2

Query: 703 HLIPKXSNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPT 524
           HLIP  +  ESKVFY KMKGDYYRYLAE   GE      + S +AY+ A +I+ A++ PT
Sbjct: 108 HLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPT 167

Query: 523 HPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRD 344
            P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L+E+SYKDSTLIMQLLRD
Sbjct: 168 DPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKDSTLIMQLLRD 227

Query: 343 NLTLWTSDTQ 314
           NLTLWTSD +
Sbjct: 228 NLTLWTSDAE 237


>SPAC23H3.05c |swd1||COMPASS complex subunit
           Swd1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 398

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
 Frame = +2

Query: 161 LASASVMIPRQTTFSNVTRFLI-NTDENKCVCWGECGEILVVAA 289
           L + SV+I   +TFS V+R L  +T   + VCW  C   L+ A+
Sbjct: 43  LVNGSVVIWDLSTFS-VSRVLTGHTRAIQSVCWSSCDRFLLTAS 85


>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1207

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
 Frame = -2

Query: 634  RYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 488
            RY+  ++   E R    ED+++A + ++  + A +QP H  I L LA+N S
Sbjct: 1049 RYIVALSCELERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099


>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 817

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 2/81 (2%)
 Frame = -2

Query: 577 QKAYQDAFEISKAKMQPTHPIRL-GLALNF-SVFYYEILNSPDKACQLAKQAFDDAIAEL 404
           Q+   D  E   +   P H        L F S  Y ++    D   QLA Q F     ++
Sbjct: 415 QRLVADLHEFYNSVKGPAHSFETEDPVLKFVSQSYDDLFPYMDNFIQLAVQLFYHISKKV 474

Query: 403 DTLNEDSYKDSTLIMQLLRDN 341
           + L  +++  S  ++Q ++DN
Sbjct: 475 NCLYVEAFSSSDAVLQRIQDN 495


>SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 85

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = +2

Query: 524 CGLHFRLADFKSILVCFL*ILYNRMSGFSCGHFC 625
           C L+F L + K+ L+    I+Y  + GFS  H C
Sbjct: 46  CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75


>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 971

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 13/42 (30%), Positives = 18/42 (42%)
 Frame = -2

Query: 703 HLIPKXSNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 578
           H     SNP S     K KG++  Y    A G    ++ +DS
Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,611,196
Number of Sequences: 5004
Number of extensions: 49730
Number of successful extensions: 156
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)