Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P05_pT_E23
(779 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 74 2e-14
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 69 6e-13
SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 65 9e-12
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 49 7e-07
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 42 1e-04
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 30 0.43
SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyc... 28 1.7
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 27 3.0
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 27 3.0
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 27 4.0
SPAC27F1.05c |||aminotransferase class-III, unknown specificty|S... 26 5.3
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 5.3
SPAC823.13c |||mitochondrial inner membrane protein|Schizosaccha... 26 7.0
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 26 7.0
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 26 7.0
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 26 7.0
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 26 7.0
SPAC3G9.07c |hos2|hda1, phd1|histone deacetylase |Schizosaccharo... 25 9.2
SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces po... 25 9.2
SPAC17H9.12c |||NADH-cytochrome reductase|Schizosaccharomyces po... 25 9.2
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 9.2
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 74.1 bits (174), Expect = 2e-14
Identities = 42/101 (41%), Positives = 58/101 (57%), Gaps = 2/101 (1%)
Frame = -3
Query: 369 DPEWICSKEKPN-YDRIFQSLNPIDGKVTGAAAKTEMV-KSKLPNSVLGKIWKLSDIDKD 196
D W SKE+ YD+IF + + G A E+ +SKL + L IW L D
Sbjct: 812 DIPWAISKEEKRIYDQIFDAWDKERKGTLGGNAVLEIFGQSKLTRTELEHIWNLCDHGDK 871
Query: 195 GFLDEEEFALAMHLISVKVDGHDLPTELPSHLVPPSKRN*T 73
G LD +EFA+A+HLI K++G+++P LP L+PPS RN T
Sbjct: 872 GSLDRDEFAVALHLIYRKLNGNEVPAVLPPELIPPSTRNFT 912
Score = 55.2 bits (127), Expect = 1e-08
Identities = 25/88 (28%), Positives = 52/88 (59%)
Frame = -3
Query: 360 WICSKEKPNYDRIFQSLNPIDGKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDE 181
++ + ++ ++++F+S + ++ K +V+SKLP L KIW+LSD + G L
Sbjct: 276 FVSAADQAKFEQLFKSAVGREEAMSSEIGKAILVRSKLPTVQLSKIWRLSDTTRSGRLLF 335
Query: 180 EEFALAMHLISVKVDGHDLPTELPSHLV 97
+F LAM+L ++ + G +P ++P ++
Sbjct: 336 PQFVLAMYLCNLGLTGKPIPDKVPDGIL 363
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 69.3 bits (162), Expect = 6e-13
Identities = 37/92 (40%), Positives = 55/92 (59%), Gaps = 2/92 (2%)
Frame = -3
Query: 348 KEKPNYDRIFQSLNPIDGKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDEEEFA 169
+EK Y IF+ LNP +G ++G+ A + SKL + L KIW L+DID DG D +EFA
Sbjct: 4 QEKNKYWEIFRGLNPENGYLSGSKAAGVLRSSKLSSDKLEKIWDLADIDDDGMFDFDEFA 63
Query: 168 LAMHLISVKVDG--HDLPTELPSHLVPPSKRN 79
+AM + ++G +P +P LV SK++
Sbjct: 64 IAMKITFDLINGVYKTVPDRVPEALVSTSKKH 95
Score = 34.7 bits (76), Expect = 0.015
Identities = 20/93 (21%), Positives = 42/93 (45%)
Frame = -3
Query: 360 WICSKEKPNYDRIFQSLNPIDGKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDE 181
+I +K Y I+ G+V+ +PNS + K WKL + + +
Sbjct: 134 YISPSDKIRYSDIYSLHCNKHGEVSFNGLAPVFTPFGVPNSQIQKAWKLVNPQGTETIQK 193
Query: 180 EEFALAMHLISVKVDGHDLPTELPSHLVPPSKR 82
++ + +H+++ + +G +P ++P L KR
Sbjct: 194 DQCLVFLHILTQRSNGFRIPNDVPYSLKASFKR 226
>SPBC800.10c |||EPS15 repeat family actin cortical patch component
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1116
Score = 65.3 bits (152), Expect = 9e-12
Identities = 33/92 (35%), Positives = 59/92 (64%), Gaps = 1/92 (1%)
Frame = -3
Query: 369 DPEWICSKEKPNYDRIFQSLNPID-GKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDG 193
DP I S++ ++ ++F +++ G V+G+ A + + SKLP VL +IW LSD + +G
Sbjct: 251 DPWAIPSQDLTSFCQLFSNVDKAHKGYVSGSEAYSFFLASKLPEDVLAQIWDLSDTNSNG 310
Query: 192 FLDEEEFALAMHLISVKVDGHDLPTELPSHLV 97
L+ EF ++++LI +K+ G +LP LPS ++
Sbjct: 311 KLNIGEFCISLYLIKLKLSGKELPKVLPSSML 342
Score = 56.8 bits (131), Expect = 3e-09
Identities = 30/94 (31%), Positives = 47/94 (50%)
Frame = -3
Query: 414 PFGYGRGEGVDAGHGDPEWICSKEKPNYDRIFQSLNPIDGKVTGAAAKTEMVKSKLPNSV 235
P G+G D P I S E Y ++F ++ P DG + G A + ++ L +
Sbjct: 113 PNNVSSGDGSDGSFLPP--ISSDEMTRYQQMFTTVCPTDGLMDGDRASSIFGRAPLSTEI 170
Query: 234 LGKIWKLSDIDKDGFLDEEEFALAMHLISVKVDG 133
L ++W L D K G LD EF MH+I++ ++G
Sbjct: 171 LARVWNLVDTHKRGALDIREFNTGMHIINLLLNG 204
Score = 48.8 bits (111), Expect = 9e-07
Identities = 24/72 (33%), Positives = 44/72 (61%), Gaps = 1/72 (1%)
Frame = -3
Query: 357 ICSKEKPNYDRIFQSLNPID-GKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDE 181
+ ++E+ +D++F+ + D G +TG A + KS L VLG+IW+++D + GFL
Sbjct: 5 LSAEEQTAFDQLFKIADKQDIGVITGEEAVPFLEKSGLAPQVLGQIWQIADAENRGFLTF 64
Query: 180 EEFALAMHLISV 145
F +AM L+++
Sbjct: 65 SGFVIAMRLVAL 76
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 49.2 bits (112), Expect = 7e-07
Identities = 24/83 (28%), Positives = 45/83 (54%), Gaps = 1/83 (1%)
Frame = -3
Query: 357 ICSKEKPNYDRIFQSL-NPIDGKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDE 181
I +++ N+ ++F + N G + G A + S L + L +IW D G++D+
Sbjct: 296 ITPEDRSNFYQLFSKIDNENKGYIVGGEAVPFFMASHLDSEELARIWDTVDTQDRGYIDK 355
Query: 180 EEFALAMHLISVKVDGHDLPTEL 112
+EFA+AM +I +++ G L + L
Sbjct: 356 DEFAVAMEIIKLRLSGKSLASIL 378
Score = 43.2 bits (97), Expect = 4e-05
Identities = 22/73 (30%), Positives = 43/73 (58%), Gaps = 2/73 (2%)
Frame = -3
Query: 297 GKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDEEEFALAMHLISV--KVDGHDL 124
G + + AK ++KS L +VL +IW L+D G+L+ ++ +A HL+++ + + L
Sbjct: 151 GCMPSSIAKPILLKSSLHYAVLAQIWNLADSAHVGYLEMYQYVIARHLVAICKQYNLIHL 210
Query: 123 PTELPSHLVPPSK 85
P LP+ ++ +K
Sbjct: 211 PRSLPADVIESAK 223
Score = 40.3 bits (90), Expect = 3e-04
Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
Frame = -3
Query: 345 EKPNYDRIFQSLNPIDGK-VTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDEEEFA 169
E+ +DR+ +SL+P + G+ ++K LP +L KIW D + G LD +
Sbjct: 10 EQQEFDRLLESLHPEQKDLIPGSVIGPFLLKFGLPQKILAKIWDYCDQEDKGSLDRNQVY 69
Query: 168 LAMHLIS 148
LIS
Sbjct: 70 ACFRLIS 76
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1379
Score = 41.9 bits (94), Expect = 1e-04
Identities = 23/59 (38%), Positives = 31/59 (52%)
Frame = -3
Query: 258 KSKLPNSVLGKIWKLSDIDKDGFLDEEEFALAMHLISVKVDGHDLPTELPSHLVPPSKR 82
+S P SVL KI+ L D G L +EF + M LI + G LP ++P + SKR
Sbjct: 1270 RSGAPTSVLAKIYDLVDRHHTGVLGRDEFIVGMFLIDQYLKGRKLPLKVPDSVWLSSKR 1328
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 29.9 bits (64), Expect = 0.43
Identities = 22/73 (30%), Positives = 38/73 (52%), Gaps = 2/73 (2%)
Frame = +2
Query: 344 SLLQIHSGSPWPASTPSPRP*PNGETWSSTPSKAPPF--IRGSLTMLMSSCGIISMSRAM 517
S Q ++ S P+STPS P + + ++T + + P + + T SS + S+S A
Sbjct: 272 SSAQYNTSSLLPSSTPSSTPLSSANSTTATSASSTPLTSVNSTTTTSASSTPLSSVSSAN 331
Query: 518 SVASMWSTTSSNL 556
S + ST+S+ L
Sbjct: 332 STTAT-STSSTPL 343
>SPAC4G9.05 |mpf1||meiotic PUF family protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 27.9 bits (59), Expect = 1.7
Identities = 16/55 (29%), Positives = 33/55 (60%)
Frame = -3
Query: 756 IVNELRKEMPSMFGKDSKKKDXIKNLGQVYDKIQREQQISPGDFPDIKKMQETLA 592
+V+E +K++ S + KKKD I+ L ++Y+ + + ++ P +K + ETL+
Sbjct: 40 LVSEQQKKIESSTLEVQKKKDQIRELERIYE-MNPKSFLTLRRTPALKLLPETLS 93
>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1260
Score = 27.1 bits (57), Expect = 3.0
Identities = 14/53 (26%), Positives = 26/53 (49%)
Frame = +1
Query: 127 VVSVHLHRDEMHRERKLLFVQEAVLVDVGELPNLAEHRVGQLRLHHLCLCGGA 285
++++ + +++ E + F+Q A + L E R L HHL + GGA
Sbjct: 432 IINIDIEKEKTIEEIAMGFIQVANETMCRPIRKLTESRGLDLSAHHLAVFGGA 484
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 27.1 bits (57), Expect = 3.0
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = -3
Query: 753 VNELRKEMPSMFGKDS--KKKDXIKNLGQVYDKIQREQQ 643
V E RKE + K+ KKK+ +K G+ K Q+EQQ
Sbjct: 284 VEEARKEEARLKKKEKERKKKEEMKAQGKYLSKKQKEQQ 322
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 26.6 bits (56), Expect = 4.0
Identities = 10/33 (30%), Positives = 21/33 (63%)
Frame = -3
Query: 246 PNSVLGKIWKLSDIDKDGFLDEEEFALAMHLIS 148
P++ ++ + D DK+G++D +EF A+ + S
Sbjct: 61 PSAFAEYVFNVFDADKNGYIDFKEFICALSVTS 93
Score = 25.8 bits (54), Expect = 7.0
Identities = 17/55 (30%), Positives = 29/55 (52%)
Frame = -3
Query: 336 NYDRIFQSLNPIDGKVTGAAAKTEMVKSKLPNSVLGKIWKLSDIDKDGFLDEEEF 172
+YD + + ++ I K+ G+ K + P + KI+ + D +KDG L EEF
Sbjct: 117 SYDEMLRIVDAIY-KMVGSMVKLPEDEDT-PEKRVNKIFNMMDKNKDGQLTLEEF 169
>SPAC27F1.05c |||aminotransferase class-III, unknown
specificty|Schizosaccharomyces pombe|chr 1|||Manual
Length = 484
Score = 26.2 bits (55), Expect = 5.3
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -3
Query: 357 ICSKEKPNYDRIFQSLNPIDGKVTGA 280
+ ++ KPN + +LN GK TGA
Sbjct: 172 LATRYKPNKKKFLSTLNSFHGKTTGA 197
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 26.2 bits (55), Expect = 5.3
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3
Query: 393 EGVDAGHGDPEWICSKEKPNYD 328
E V +GD E IC K KP++D
Sbjct: 318 EIVKIWYGDEEKICKKAKPSFD 339
>SPAC823.13c |||mitochondrial inner membrane
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 317
Score = 25.8 bits (54), Expect = 7.0
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = -3
Query: 219 KLSD-IDKDGFLDEEEFALAMHLISVKVDGHDLPTELPSHLVPP 91
K+S+ +DK G E A+ L S K+D +LP S ++PP
Sbjct: 216 KISEKLDKKG----EAVGQALELTSPKIDNKNLPKNSASLILPP 255
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 25.8 bits (54), Expect = 7.0
Identities = 12/28 (42%), Positives = 15/28 (53%), Gaps = 2/28 (7%)
Frame = +1
Query: 16 FNKLQIX*MCWSRARGTAARSV--PFGG 93
F+K ++ CW R RG A V P GG
Sbjct: 185 FHKYRVKRNCWPRTRGVAMNPVDHPHGG 212
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 25.8 bits (54), Expect = 7.0
Identities = 12/28 (42%), Positives = 15/28 (53%), Gaps = 2/28 (7%)
Frame = +1
Query: 16 FNKLQIX*MCWSRARGTAARSV--PFGG 93
F+K ++ CW R RG A V P GG
Sbjct: 185 FHKYRVKRNCWPRTRGVAMNPVDHPHGG 212
>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
Win1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1436
Score = 25.8 bits (54), Expect = 7.0
Identities = 19/75 (25%), Positives = 39/75 (52%), Gaps = 4/75 (5%)
Frame = +1
Query: 127 VVSVHLHRDEMHRERKLLFVQEAVLVDVGELPNLAEH---RVGQLRLH-HLCLCGGASHF 294
V V LH+ + R E +++++ + PN+ + V + +++ + LC G+S F
Sbjct: 1148 VKEVALHKPRISRPMIKRIKGEMLVLELFDHPNVVSYYGIEVHREKVNIFMELCQGSSLF 1207
Query: 295 AVDRVQRLEDSVVVR 339
R R+ED +V++
Sbjct: 1208 EFLRYGRIEDELVIQ 1222
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 25.8 bits (54), Expect = 7.0
Identities = 12/28 (42%), Positives = 15/28 (53%), Gaps = 2/28 (7%)
Frame = +1
Query: 16 FNKLQIX*MCWSRARGTAARSV--PFGG 93
F+K ++ CW R RG A V P GG
Sbjct: 185 FHKYRVKRNCWPRTRGVAMNPVDHPHGG 212
>SPAC3G9.07c |hos2|hda1, phd1|histone deacetylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 25.4 bits (53), Expect = 9.2
Identities = 7/28 (25%), Positives = 17/28 (60%)
Frame = -3
Query: 609 MQETLANHDFTKFHPLKPKLLEVVDHML 526
+ E + N+ + HP+KP + + +H++
Sbjct: 32 LDEQVGNYHYGDKHPMKPHRITITNHLV 59
>SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 228
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = +2
Query: 410 NGETWSSTPSKAPPFIRGSLTMLMSSCGIISMSRAMSVASMWS 538
NG+ +T S PP R ++S C + A+ VA + S
Sbjct: 99 NGKALMATGSPLPPLTRNGKEYVISQCNNALLYPALGVACVLS 141
>SPAC17H9.12c |||NADH-cytochrome reductase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 266
Score = 25.4 bits (53), Expect = 9.2
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -3
Query: 312 LNPIDGKVTGAAAKTEMVKSKLPNSVLGKIWKL 214
++ I G A+ VK L N+ GK+WKL
Sbjct: 234 VSTIAGPKADYGARQGEVKGLLSNNPFGKVWKL 266
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.4 bits (53), Expect = 9.2
Identities = 8/20 (40%), Positives = 17/20 (85%)
Frame = -3
Query: 270 TEMVKSKLPNSVLGKIWKLS 211
+++V+ +LPN + G+IW+L+
Sbjct: 214 SKLVRIELPNKLRGEIWELT 233
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,012,499
Number of Sequences: 5004
Number of extensions: 63499
Number of successful extensions: 231
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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