Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P05_pT_B18
(604 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 207 8e-55
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 49 5e-07
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 44 1e-05
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 37 0.002
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 36 0.006
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 36 0.006
SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos... 34 0.014
SPBC6B1.08c |ofd1||2-oxoglutarate and Fe|Schizosaccharomyces pom... 27 2.1
SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subu... 27 2.8
SPCC663.10 |||methyltransferase, DUF1613 family |Schizosaccharom... 26 3.7
SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 26 4.9
SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|... 26 4.9
SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ... 26 4.9
SPBC1604.06c |||CBF/Mak21 family|Schizosaccharomyces pombe|chr 2... 25 6.4
SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein Pop1... 25 8.5
SPBC660.07 |ntp1||alpha,alpha-trehalase Ntp1|Schizosaccharomyces... 25 8.5
SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalyti... 25 8.5
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 207 bits (506), Expect = 8e-55
Identities = 106/209 (50%), Positives = 139/209 (66%), Gaps = 21/209 (10%)
Frame = -3
Query: 584 FTEMFRGXRDQFASEIATVGQQYRVEPFKFLDPP-LKLEFPQAVQMLKEAG--------- 435
F +F+ R+++A ++A V QQY E F D ++ F AV++LKEAG
Sbjct: 368 FLYIFKTIREKYAKQVAVVRQQYPSEDFILPDADRIRFHFKDAVKLLKEAGYRKQLVPGQ 427
Query: 434 -VSVGDE---------DDLSTPDEKLLGRLVRAKYDTDFYILDKYPLAVRPFYTMPDPGN 285
V +E DD STP+E+ LG++VR KY TDFY++DKYP +VRPFYTMPDP +
Sbjct: 428 KVPEDEEFHYCEDPEFDDFSTPEERALGQIVREKYHTDFYVIDKYPSSVRPFYTMPDPED 487
Query: 284 PKYSNSYDMFMRGEEILSGAQRIHDPDLLAERARLHGVDTS-KIAAYIESFRLGCPPHAG 108
P+YSNSYD FM+G+EI+SGAQRIHDP+LL ER + GV + YI++F +GCPPHAG
Sbjct: 488 PRYSNSYDFFMKGQEIMSGAQRIHDPELLVERMKALGVSPDVGLQQYIDAFAIGCPPHAG 547
Query: 107 GGIGLERVVMLYLGLDNIKKDLNVPERPE 21
GGIGLERVVM YL L NI+ + P P+
Sbjct: 548 GGIGLERVVMFYLNLPNIRLASSFPRDPK 576
Score = 25.0 bits (52), Expect = 8.5
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = -1
Query: 52 RKTSMFPRDPKRXTP 8
R S FPRDPKR P
Sbjct: 566 RLASSFPRDPKRLLP 580
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 49.2 bits (112), Expect = 5e-07
Identities = 34/137 (24%), Positives = 60/137 (43%), Gaps = 3/137 (2%)
Frame = -3
Query: 488 PPLKLEFPQAVQMLKEAGVSV--GDEDDLSTPDEKLLGRLVRAKYDTDFYILDKYPLAVR 315
P ++L + A++ L E + G++ + R + + + + L +PL ++
Sbjct: 407 PFMRLSYEDAIKYLNEHNILTPEGEQHKFGDDIAEAAERKMTDQINRPIF-LTYFPLEIK 465
Query: 314 PFYTMPDPGNPKYSNSYDMFMRGE-EILSGAQRIHDPDLLAERARLHGVDTSKIAAYIES 138
FY P+ + S D M EI+ G+ RI D L + G+D + + E
Sbjct: 466 SFYMKRVVDRPELTESVDCLMPNVGEIVGGSMRISDIQELLAAYKREGIDPAPYYWFTEQ 525
Query: 137 FRLGCPPHAGGGIGLER 87
+ G H G G+GLER
Sbjct: 526 RKYGTTEHGGCGLGLER 542
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 44.4 bits (100), Expect = 1e-05
Identities = 31/112 (27%), Positives = 52/112 (46%), Gaps = 4/112 (3%)
Frame = -3
Query: 353 DFYILDKYPLAVRPFYTMPDPGNPKYSNSYDMFMRGEEILSGAQRIHDPDL----LAERA 186
D ++L+K PL+VR + YD+ + G E+ G+ RIH+PD+ L +
Sbjct: 478 DVHLLEKKPLSVRGLH-------------YDIVVNGIELGGGSIRIHNPDIQRFVLKDVL 524
Query: 185 RLHGVDTSKIAAYIESFRLGCPPHAGGGIGLERVVMLYLGLDNIKKDLNVPE 30
+L + I GCPPH G +G +R+ L I++ + P+
Sbjct: 525 KLPENRYATFEHLIRVLSSGCPPHGGIALGFDRLAALLTNAPGIREVIAFPK 576
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 37.1 bits (82), Expect = 0.002
Identities = 35/130 (26%), Positives = 64/130 (49%), Gaps = 2/130 (1%)
Frame = -3
Query: 416 DDLSTPDEKLLGRLVRAKYDTDFYILDKYPLAVRPFYTMPDPGNPKYSNSYDMFM-RGEE 240
+DLS+ EK L ++ + T ++ D YP ++PFY M G P + D+ + + E
Sbjct: 317 NDLSSEHEKYLCEIL---HKTPVFVTD-YPQKIKPFY-MKSSG-PDTVAAVDLLVPQVGE 370
Query: 239 ILSGAQRIHDPDLLAERARLHGVDTSKIAAYIESFRLGCPPHAGGGIGLERVVMLYLGLD 60
+ G+ R D L E + ++ Y++ + PH G G+G+ER++ G +
Sbjct: 371 LAGGSLR---KDHLDE----YKTYPPELQWYLDLMKYSNAPHGGFGLGIERLIAFLEGEN 423
Query: 59 -NIKKDLNVP 33
N+K+ + P
Sbjct: 424 TNVKETIPFP 433
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 35.5 bits (78), Expect = 0.006
Identities = 21/89 (23%), Positives = 43/89 (48%), Gaps = 9/89 (10%)
Frame = -3
Query: 281 KYSNSYDMFMRGEEILSGAQRIHDPDLLAERARLHGVDTSKI---------AAYIESFRL 129
+ S +++++ G EI + + +DP + + D ++ + ++ +
Sbjct: 435 RISKRFELYIGGYEICNAYEEENDPVAQYHKFQAQKYDRLQLGDDETPAPDSDFVHALEY 494
Query: 128 GCPPHAGGGIGLERVVMLYLGLDNIKKDL 42
G PP AG G+G++R+VML G I + L
Sbjct: 495 GLPPTAGWGMGVDRLVMLMTGQSKISEIL 523
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 35.5 bits (78), Expect = 0.006
Identities = 42/175 (24%), Positives = 76/175 (43%), Gaps = 15/175 (8%)
Frame = -3
Query: 488 PPLKLEFPQAVQMLKEA----GVSVGDEDDLSTPDEKLLGRLVRAKYDTDFYILDKYPLA 321
P +L P+A L++ GV S +KL+G + ++ +I+ +P
Sbjct: 414 PGDQLHTPEANAFLRDLCAKHGVECAPPQTCSRLLDKLVGEFIESECINPTFIIG-HPQM 472
Query: 320 VRPF--YTMPDPGNPKYSNSYDMFMRGEEILSGAQRIHDP-DLLAE-----RARLHGVDT 165
+ P Y D G ++ F+ +EI + ++D D A R + G D
Sbjct: 473 MSPLAKYHRSDAG---LCERFEAFVATKEICNAYTELNDIFDQRARFEEQARQKAQGDDE 529
Query: 164 SKIAA--YIESFRLGCPPHAGGGIGLERVVMLYLGLDNIKKDLNVPE-RPETRHA 9
++I + + G PP G G+G++R+VM + I++ L P +PE + A
Sbjct: 530 AQIIDENFCTALEYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFPHMKPEVQAA 584
>SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 482
Score = 34.3 bits (75), Expect = 0.014
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = +2
Query: 377 ACPGASRPEWTGRPRLQQTLQLPSASGRPAETPASGEDPRI 499
ACP + P + G+PR+++T +P + + E PA G+ I
Sbjct: 197 ACPTNADPNYDGKPRVKRTTGIPRSFLKNVERPAEGDAANI 237
>SPBC6B1.08c |ofd1||2-oxoglutarate and Fe|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 515
Score = 27.1 bits (57), Expect = 2.1
Identities = 10/33 (30%), Positives = 18/33 (54%)
Frame = -3
Query: 362 YDTDFYILDKYPLAVRPFYTMPDPGNPKYSNSY 264
+D + +DK +A+ ++ P PG P Y +Y
Sbjct: 210 HDVEEVYVDKPRMAISGWFHYPQPGEPGYDANY 242
>SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subunit
Apc13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 135
Score = 26.6 bits (56), Expect = 2.8
Identities = 17/61 (27%), Positives = 32/61 (52%), Gaps = 2/61 (3%)
Frame = +3
Query: 324 ERIFVQDIEVRVVLGPHQPAQELLVR--SGQVVLVSNRHSSFLQHLDGLRKLQLQGRIQE 497
+R+ V D+EVRV P E+ ++ S ++L+ N+ L+H + L+L+ +
Sbjct: 25 DRLPVDDVEVRVEHLPPVTEDEMTIQHSSANLILMKNKQ---LRHEPAWKDLELEDLVNA 81
Query: 498 F 500
F
Sbjct: 82 F 82
>SPCC663.10 |||methyltransferase, DUF1613 family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 502
Score = 26.2 bits (55), Expect = 3.7
Identities = 15/59 (25%), Positives = 25/59 (42%)
Frame = -3
Query: 428 VGDEDDLSTPDEKLLGRLVRAKYDTDFYILDKYPLAVRPFYTMPDPGNPKYSNSYDMFM 252
+G+ D TP +L RL + Y + + +P P NPK+ Y M++
Sbjct: 367 IGNHADELTPYLPILARLNQKCYFMSIPCCVHDLTGAKISWALPKPRNPKHGGRYAMYI 425
>SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 682
Score = 25.8 bits (54), Expect = 4.9
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3
Query: 396 VRSGQVVLVSNRHSSFLQHLDGL 464
+ SG VVL+ +HS Q LD +
Sbjct: 115 LESGSVVLIQKQHSKLRQRLDSI 137
>SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase
Snf21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1199
Score = 25.8 bits (54), Expect = 4.9
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -3
Query: 419 EDDLSTPDEKLLGRLVRAKYDTDFYILDKYPLAV 318
EDD P KL L K D+Y++ K P+A+
Sbjct: 1081 EDDNGRPLNKLFLELPSKKLYPDYYMIIKSPIAL 1114
>SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 429
Score = 25.8 bits (54), Expect = 4.9
Identities = 11/26 (42%), Positives = 18/26 (69%)
Frame = -3
Query: 104 GIGLERVVMLYLGLDNIKKDLNVPER 27
GIGLER+ M+ G+ +I+ ++ ER
Sbjct: 297 GIGLERLAMILYGIPDIRLFWSLDER 322
>SPBC1604.06c |||CBF/Mak21 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 485
Score = 25.4 bits (53), Expect = 6.4
Identities = 11/31 (35%), Positives = 14/31 (45%)
Frame = -3
Query: 467 PQAVQMLKEAGVSVGDEDDLSTPDEKLLGRL 375
P +QML + GD D PD L G +
Sbjct: 377 PTCMQMLHRSSAESGDSFDFDQPDPLLTGAI 407
>SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein
Pop1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 775
Score = 25.0 bits (52), Expect = 8.5
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +3
Query: 330 IFVQDIEVRVVLGPHQPAQELLVRSGQVV 416
IF Q E +VV G H Q +RSG++V
Sbjct: 704 IFFQHDESKVVSGSHSTLQLWDIRSGKLV 732
>SPBC660.07 |ntp1||alpha,alpha-trehalase Ntp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 735
Score = 25.0 bits (52), Expect = 8.5
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -1
Query: 427 LETRTTCPLRTRSSWAGWCGPSTTRTSISWTNILSP 320
LET++T T + WA W G +T R + + ++ P
Sbjct: 540 LETKSTYESAT-AFWALWAGVATPRQAAKFVDVSLP 574
>SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalytic
subunit Cdc6 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1086
Score = 25.0 bits (52), Expect = 8.5
Identities = 17/58 (29%), Positives = 28/58 (48%)
Frame = +3
Query: 333 FVQDIEVRVVLGPHQPAQELLVRSGQVVLVSNRHSSFLQHLDGLRKLQLQGRIQEFEG 506
FV E+ V G P LL R Q+ ++S LQH + +++ G +++EG
Sbjct: 513 FVNYTEMARVTGV--PFNFLLARGQQIKVISQLFRKALQHDLVVPNIRVNGTDEQYEG 568
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,624,149
Number of Sequences: 5004
Number of extensions: 56468
Number of successful extensions: 216
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 213
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -