Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P05_pT_B13
(716 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 90 3e-19
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 5e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 55 1e-08
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 52 1e-07
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 36 0.006
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 35 0.010
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.018
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 32 0.071
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 32 0.094
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 28 1.5
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 27 2.0
SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 2.0
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 2.7
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 27 3.5
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 26 6.2
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 26 6.2
SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 8.2
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 25 8.2
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 90.2 bits (214), Expect = 3e-19
Identities = 39/119 (32%), Positives = 78/119 (65%)
Frame = -3
Query: 714 KISTILXTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKE 535
++ ++ ++GQ +ELQ +I+E D + +G I+F F + + +++ D E +E++E
Sbjct: 33 ELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNE---EEVRE 89
Query: 534 AFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMT 358
AF+++D++GNGYIT L +L +L ++LS ++ +I E DTDG G ++++EF +++
Sbjct: 90 AFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVIS 148
Score = 62.5 bits (145), Expect = 6e-11
Identities = 30/73 (41%), Positives = 49/73 (67%)
Frame = -3
Query: 546 ELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 367
E +EAF L+DR+ +G IT++ L ++ +L + A+L +I E+D DG+GT+DF EF+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 366 MMTGD*AQRSKDS 328
MM A++ KD+
Sbjct: 73 MM----ARKMKDT 81
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 56.0 bits (129), Expect = 5e-09
Identities = 32/115 (27%), Positives = 65/115 (56%)
Frame = -3
Query: 711 ISTILXTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEA 532
+ ++L ++G D+EL L +E I+ F + S+ L E ++E +E +A
Sbjct: 31 VGSVLRSLGINVTDAELAKLSNEL----GDAIDEKKFMSFVSNKLRETESE---EEYIKA 83
Query: 531 FRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 367
FR++D++ +GYI T+ + + L +KLS+ ++ ++ E D SG+ D+ +F++
Sbjct: 84 FRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Score = 35.9 bits (79), Expect = 0.006
Identities = 20/63 (31%), Positives = 37/63 (58%)
Frame = -3
Query: 546 ELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 367
E+KEAF LYD + +G I TS + +L +L +++A+L A++ + +D +FM
Sbjct: 10 EMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAEL----AKLSNELGDAIDEKKFMS 65
Query: 366 MMT 358
++
Sbjct: 66 FVS 68
Score = 35.1 bits (77), Expect = 0.010
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = -3
Query: 714 KISTILXTMGQLFDDSELQALIDENDPENSGKINFDGF 601
K + + T+G+ D+E+Q ++ E DP NSG ++ F
Sbjct: 99 KFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 54.8 bits (126), Expect = 1e-08
Identities = 27/118 (22%), Positives = 59/118 (50%)
Frame = -3
Query: 714 KISTILXTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKE 535
++ + +G + SE+ ++ + D G + + F + + + E D +E+K
Sbjct: 58 ELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDP---LEEIKR 114
Query: 534 AFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 361
AF L+D + G I+ L+ + L++ + + +L+ +I E D D G ++ EF+ +M
Sbjct: 115 AFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIM 172
Score = 42.3 bits (95), Expect = 7e-05
Identities = 20/74 (27%), Positives = 39/74 (52%)
Frame = -3
Query: 579 LEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDG 400
L E E +Q++ EAF+L+D + + I L+ + AL +++ I+ + D G
Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTG 86
Query: 399 SGTVDFDEFMEMMT 358
G + ++F+ +MT
Sbjct: 87 KGYLQMEDFVRVMT 100
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 51.6 bits (118), Expect = 1e-07
Identities = 34/111 (30%), Positives = 60/111 (54%), Gaps = 6/111 (5%)
Frame = -3
Query: 669 SELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITT 490
S L +++DE + G ++F F N S F + E ++LK AF++YD + +GYI+
Sbjct: 59 SRLFSVVDE---DGGGDVDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISN 112
Query: 489 STLKEILAAL------DDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMTG 355
L +L + +D+L +D I E+D D G + F+EF ++++G
Sbjct: 113 GELYLVLKMMVGTNLREDQLQQI-VDKTIMEVDKDRDGKISFEEFKDIVSG 162
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/64 (23%), Positives = 31/64 (48%)
Frame = -3
Query: 549 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFM 370
+ +++ F D +G I + I + + L++ + + +D DG G VDF EF+
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLASR----LFSVVDEDGGGDVDFQEFI 79
Query: 369 EMMT 358
++
Sbjct: 80 NSLS 83
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.9 bits (79), Expect = 0.006
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = -3
Query: 549 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 409
QELKEAF L D++G+G I +K +L +L+ S ++ + I+
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 35.1 bits (77), Expect = 0.010
Identities = 18/60 (30%), Positives = 35/60 (58%)
Frame = -3
Query: 540 KEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 361
K+AF L+DR G G I +++ ++L A + A+ I EI++ VD ++F++++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQVL 64
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 34.3 bits (75), Expect = 0.018
Identities = 21/64 (32%), Positives = 32/64 (50%)
Frame = -3
Query: 555 MQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDE 376
M +E +EAF L+D GYI L+ A L + L+ L ++ T+G V +E
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK--VSREE 67
Query: 375 FMEM 364
F E+
Sbjct: 68 FAEL 71
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 32.3 bits (70), Expect = 0.071
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -3
Query: 573 EEDAEAMQQELKEAFRLYDREGNGYITTSTLKE 475
++D M Q LKE Y+ E N IT+ TLKE
Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 31.9 bits (69), Expect = 0.094
Identities = 22/55 (40%), Positives = 29/55 (52%)
Frame = -3
Query: 537 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEF 373
E FR + E NGY++ S +L + KLS+ L+ I D D G DFDEF
Sbjct: 11 EIFRGLNPE-NGYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGMFDFDEF 62
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 27.9 bits (59), Expect = 1.5
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = -3
Query: 711 ISTILXTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHF 580
+ST+ T + DDS L+ +ID+ PE GK NI++HF
Sbjct: 14 VSTVEDTSFEAMDDSILE-IIDDEAPEIQGKPK----ANISTHF 52
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/69 (24%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = -3
Query: 690 MGQLFDDSELQALIDENDP--ENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYD 517
+G+ D ++ + + +P ENS N D +A+ FL+E + E ++E + + L
Sbjct: 414 IGKYLGDKDIFQIENFINPIEENSADTNDDIVNQVAAQFLDEREFETDEEEEESQYLLST 473
Query: 516 REGNGYITT 490
++ I T
Sbjct: 474 KDAINAINT 482
>SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 418
Score = 27.5 bits (58), Expect = 2.0
Identities = 15/68 (22%), Positives = 29/68 (42%)
Frame = -3
Query: 651 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEI 472
+ E + F F + ++ +D A+ Q + +F +R G G I T ++
Sbjct: 343 VSEESQVEPNTLIFQNFSSPSTSLSAPKDTPAVIQSINTSFLNNERAGRGNIKTVDQNQL 402
Query: 471 LAALDDKL 448
+ + DKL
Sbjct: 403 INRIVDKL 410
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 27.1 bits (57), Expect = 2.7
Identities = 18/59 (30%), Positives = 23/59 (38%)
Frame = -3
Query: 537 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 361
+ F D E GYI A L + +L I +DT G +D DEF M
Sbjct: 306 QLFSKIDNENKGYIVGGEAVPFFMA--SHLDSEELARIWDTVDTQDRGYIDKDEFAVAM 362
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = -2
Query: 196 RFCLSCRNVLVIYVPASCRSRTYLNSFQRVIFGI 95
R CL+ R ++ +Y+ C + N F++++F I
Sbjct: 533 RICLNPRALVELYINYDCDRSSTTNVFEQLLFSI 566
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 25.8 bits (54), Expect = 6.2
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = -3
Query: 639 DPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL 460
D + +G I+F F S E + +L AF+LYD + NG I+ + I+ A+
Sbjct: 73 DADKNGYIDFKEFICALSVTSRGE----LNDKLIWAFQLYDLDNNGLISYDEMLRIVDAI 128
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1183
Score = 25.8 bits (54), Expect = 6.2
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = -3
Query: 525 LYDREGNGYITT-STLKEILAALDDKLSNADLDGII-AEIDTDGSGTVDFDEFMEMMTGD 352
LYD+E GY+T + E++ + D+ N D + ++ + SG V E+
Sbjct: 1055 LYDKEKRGYVTLFPSGSEVVQFVPDRTYNIDSEYVVYLSLHESRSGRVYMSPLTEISPEH 1114
Query: 351 *AQRSKDS 328
A+ +K++
Sbjct: 1115 LARLAKNT 1122
>SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 8.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -3
Query: 516 REGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 409
RE GY+ T + D L+NA+L I+A+ID
Sbjct: 107 REETGYVGTVMDSTTVMYNDPGLTNANLKIILADID 142
>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.2
Identities = 20/100 (20%), Positives = 41/100 (41%), Gaps = 5/100 (5%)
Frame = -3
Query: 651 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQ---ELKEAFRLYDREGNGYITTSTL 481
I +N P + IN + ++ EE E KE LY+++ G++ +
Sbjct: 181 IMKNSPMPAEPINKENINQLSRSLFYEEGKEFWDDYDIAAKEILSLYEQDPEGFVNPYSK 240
Query: 480 KEILAALDDKLSNADLDGIIAEI--DTDGSGTVDFDEFME 367
+ A + + + G+ A+ D D + +EF++
Sbjct: 241 RGAAALANSENNKGSEAGVSAKTNPDMDSDSSAIVNEFLK 280
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,626,188
Number of Sequences: 5004
Number of extensions: 49155
Number of successful extensions: 173
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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