Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P05_F_N13
(803 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 1.4
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 2.4
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 27 4.1
SPBC713.05 |||WD repeat protein, human MAPK organizer 1 |Schizos... 26 5.5
SPAC22E12.10c |etp1|cox15|mitochondrial type I [2Fe-2S] ferredox... 26 5.5
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 5.5
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 26 7.2
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 26 7.2
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 9.5
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.5
SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 9.5
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 9.5
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 9.5
>SPAC1006.07 |||translation initiation factor
eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 28.3 bits (60), Expect = 1.4
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +3
Query: 279 AIDLLTNDECRLLLEVEDFFN 341
+I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 412 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 501
STR + N+VP N L I+C + NK
Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103
>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 26.6 bits (56), Expect = 4.1
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = +2
Query: 41 FSCNXAITMGF*YLIVCTPACAFTHN-IKMLAKMAPS-QDKLVHLHKTPTSLDINPSGLL 214
F C I++ F I A A H I L + P +D H K I+P LL
Sbjct: 310 FVCPSCISLDFDLQI---QAFARQHRVISTLGVVYPEREDSCFHKIKAAQWHQISPRSLL 366
Query: 215 FAFVELNHYNNECESEGVMGCGHRF 289
F F E NH +++ ++ G ++
Sbjct: 367 FQFQEQNHIHHKKIRRKLLAAGWKW 391
>SPBC713.05 |||WD repeat protein, human MAPK organizer 1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 26.2 bits (55), Expect = 5.5
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 674 LWCCRVNAFNTAAESAGTRDSVAS 745
LW CR N+F+ A +DSV+S
Sbjct: 124 LWDCRSNSFSPIQVLADAKDSVSS 147
>SPAC22E12.10c |etp1|cox15|mitochondrial type I [2Fe-2S] ferredoxin
Etp1/ cytochrome oxidase cofactor Cox15,
fusion|Schizosaccharomyces pombe|chr 1|||Manual
Length = 631
Score = 26.2 bits (55), Expect = 5.5
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -3
Query: 120 ILCVKAHAGVQTIRY*KPIVIAKLHEKTKIRLLTAS 13
++ +A G+ T+ Y P+ +A LH+ + LTA+
Sbjct: 422 VVTAQATLGIMTLIYVVPVPLAALHQAGSLVTLTAA 457
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.2 bits (55), Expect = 5.5
Identities = 16/62 (25%), Positives = 25/62 (40%)
Frame = +3
Query: 543 PSIFENISEVPERVDPQPPAAVLASSPFVTSQPTEELLREFETVYGAVELTHLTPPQSPP 722
P+ S V P P A++ AS+P + S P ++ + T + P Q P
Sbjct: 895 PAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHP--PTASGTRVPPVQQPS 952
Query: 723 GP 728
P
Sbjct: 953 HP 954
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/52 (25%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +3
Query: 522 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT-EELLREFETV 674
+E + D+ S N S +P+ P V+ SSP + P+ +++ + +T+
Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPSHSDVMGQKDTI 521
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -3
Query: 696 ALTRQHHKPFRIPAAVLQWAARSQKDLK-LEPLPGVVGPRVQELQK 562
A T H P + P+ +QW +S +DL+ ++ V+ +E K
Sbjct: 746 AFTHLGHHPDKTPSEYIQWRFQSGEDLEAMDKASRVISEADEEAMK 791
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 25.4 bits (53), Expect = 9.5
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +3
Query: 480 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 596
+D CKQ+ +FS E++ D L SI +S + ++ P
Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 9.5
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 297 NDECRLLLEVEDFFNDDC 350
NDECR L + FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552
>SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 95
Score = 25.4 bits (53), Expect = 9.5
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +2
Query: 152 DKLVHLHKTPTSLDINPSGLLFAFVELNH 238
D + H K +S NPS LLF L H
Sbjct: 59 DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.4 bits (53), Expect = 9.5
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = -1
Query: 422 WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 276
W T+ V R S RKV + + + EE +++ ALI + + R
Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191
>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 304
Score = 25.4 bits (53), Expect = 9.5
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
Frame = +2
Query: 128 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 262
L + ++KLV+L T T LD + ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,971,261
Number of Sequences: 5004
Number of extensions: 57341
Number of successful extensions: 162
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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