Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P05_F_E22
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom... 52 1e-07
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.28
SPAC1782.08c |rex3||exonuclease Rex3 |Schizosaccharomyces pombe|... 29 1.1
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 2.6
SPBC1604.19c |||TRAPP complex subunit Trs85 |Schizosaccharomyces... 27 4.5
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 27 4.5
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 6.0
SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 26 7.9
SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 26 7.9
>SPBC2D10.09 |||3-hydroxyisobutyryl-CoA
hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 429
Score = 51.6 bits (118), Expect = 1e-07
Identities = 36/135 (26%), Positives = 68/135 (50%), Gaps = 4/135 (2%)
Frame = +2
Query: 260 NGVYVVTLDSPNVKVNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISM 439
NG + TL+ P V +N++N +++ + + +E S + +I+ G F +G DI
Sbjct: 64 NGARIFTLNRPKV-LNAINVDMIDSILPKLVSLE-ESNLAKVIILKGNGRSFSSGGDIKA 121
Query: 440 ----IENCKTKEEVVSLSKRGHEIFRRIEQSRKPYIAAIQGSCLGGGLETALACKYRIAV 607
I++ K E + ++ + + + +KP +A + G +GGG A+ +RIA
Sbjct: 122 AALSIQDGKLPEVRHAFAQE-YRLSHTLATYQKPVVALMNGITMGGGSGLAMHVPFRIAC 180
Query: 608 KDSKTGFGLPEVMLG 652
+D T F +PE +G
Sbjct: 181 ED--TMFAMPETGIG 193
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.7 bits (66), Expect = 0.28
Identities = 29/87 (33%), Positives = 33/87 (37%), Gaps = 4/87 (4%)
Frame = -3
Query: 810 PKPGPRGETNRXTIP--NFFALSAFTVLPVRARSKVVGMEVRA--GNLCVPPPPGKSPNM 643
P P P NR P N + S+ P RS G G PPPP +S
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPS 371
Query: 642 TSGSPNPVLESFTAMRYLHARAVSNPP 562
T P P+ S RAVSNPP
Sbjct: 372 TGRQPPPLSSS---------RAVSNPP 389
>SPAC1782.08c |rex3||exonuclease Rex3 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 540
Score = 28.7 bits (61), Expect = 1.1
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +2
Query: 239 HTKCKLVNGVYVVTLDSPNVKVNSLNTQVMEEVSNIVNEIETNSGIE 379
+ CKL V +VT P V +LN + + +S +N +TN I+
Sbjct: 63 YDNCKLSICVPIVTTCIPYHSVENLNFNIRQNISTTLNLEKTNDSIK 109
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.5 bits (58), Expect = 2.6
Identities = 22/94 (23%), Positives = 42/94 (44%)
Frame = +2
Query: 296 VKVNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKEEVVS 475
V+ + + Q +++ N + + A + I P G S + + T E VS
Sbjct: 534 VRGDDSDVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTTG--YSCVMHIHTAVEEVS 591
Query: 476 LSKRGHEIFRRIEQSRKPYIAAIQGSCLGGGLET 577
+K H++ + +S+KP + A +G + LET
Sbjct: 592 FAKLLHKLDKTNRKSKKPPMFATKGMKIIAELET 625
>SPBC1604.19c |||TRAPP complex subunit Trs85 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 658
Score = 26.6 bits (56), Expect = 4.5
Identities = 15/59 (25%), Positives = 27/59 (45%)
Frame = +1
Query: 538 RNTRQLSRRWIRDCSCM*ISHCCERLQNRIWTTRSHVGTFARRWRDTEITCSHLHTYNF 714
RNT + W SC I C +QNR + +S + ++ +W D + + ++F
Sbjct: 467 RNTFSQDKAWNYLASCEEIQIICLLMQNRNISLKSQI-SYLNKWFDEMVYIYAVRLHSF 524
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 26.6 bits (56), Expect = 4.5
Identities = 24/76 (31%), Positives = 38/76 (50%), Gaps = 2/76 (2%)
Frame = -3
Query: 609 FTAMRYLHARAVSNPPPRQLPCIAAIYGFRDCSILLNISCPLLDRL--TTSSFVLQFSIM 436
+TA+ +H + PPP+ LP I I R CS L + P+L +L T + VL+ M
Sbjct: 254 YTAVPTIHQILLRTPPPKPLPRIRFI---RSCSSPL--APPVLSKLEATFRAPVLEAYAM 308
Query: 435 LISAPAMKHPGLPDIM 388
++ M LP ++
Sbjct: 309 TEASHQMTTNPLPPLV 324
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 26.2 bits (55), Expect = 6.0
Identities = 12/40 (30%), Positives = 18/40 (45%)
Frame = -3
Query: 672 PPPPGKSPNMTSGSPNPVLESFTAMRYLHARAVSNPPPRQ 553
P P G S + S P SF + +L + SN P ++
Sbjct: 140 PRPSGNSSRLDRNSSRPFTSSFHSAPFLSSYGSSNDPSQE 179
>SPAC1296.03c |sxa2||serine carboxypeptidase
Sxa2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 25.8 bits (54), Expect = 7.9
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = -1
Query: 704 VWR*EQVISVSLHRRAKVPT*LLVVQILFWSLSQQCDIYMQEQSLIHLLDSCLVLRQYTV 525
+WR E S S R + + +F S+S CD+Y L++L +SC++ Y V
Sbjct: 294 IWRPEYNFSTSTSLRKREALDGEDIGNVFNSISG-CDLYSLSNFLLYLENSCVI--TYDV 350
Query: 524 SVIVRF 507
S+ F
Sbjct: 351 SLDCSF 356
>SPBC543.08 |||phosphoinositide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 250
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +1
Query: 235 STYKMQVGQWSLRSHFGLSK 294
S Y + V +WS+ S+FG SK
Sbjct: 38 SIYSIYVDKWSITSYFGNSK 57
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,302,047
Number of Sequences: 5004
Number of extensions: 67121
Number of successful extensions: 226
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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