Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_pT_N09
(732 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 253 2e-68
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 253 2e-68
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 253 2e-68
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 81 1e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 48 1e-06
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 33 0.042
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.6
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 6.4
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 253 bits (619), Expect = 2e-68
Identities = 115/154 (74%), Positives = 132/154 (85%)
Frame = -2
Query: 731 EVXSVEMHHEALQEAVPGDNVGFXVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 552
EV SVEMHHE+L +PGDNVGF VK VSVK++RRG V GDSKN+PP G A FTAQVI+L
Sbjct: 286 EVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIIL 345
Query: 551 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 372
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 371 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 270
KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 253 bits (619), Expect = 2e-68
Identities = 115/154 (74%), Positives = 132/154 (85%)
Frame = -2
Query: 731 EVXSVEMHHEALQEAVPGDNVGFXVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 552
EV SVEMHHE+L +PGDNVGF VK VSVK++RRG V GDSKN+PP G A FTAQVI+L
Sbjct: 286 EVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIIL 345
Query: 551 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 372
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 371 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 270
KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 253 bits (619), Expect = 2e-68
Identities = 115/154 (74%), Positives = 132/154 (85%)
Frame = -2
Query: 731 EVXSVEMHHEALQEAVPGDNVGFXVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 552
EV SVEMHHE+L +PGDNVGF VK VSVK++RRG V GDSKN+PP G A FTAQVI+L
Sbjct: 286 EVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIIL 345
Query: 551 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 372
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 371 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 270
KP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 406 KPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 81.4 bits (192), Expect = 1e-16
Identities = 47/147 (31%), Positives = 78/147 (53%), Gaps = 1/147 (0%)
Frame = -2
Query: 704 EALQEAVPGDNVGFXVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNG 525
E + ++ GD V V+ +++ GYV +KN P F AQ+ +L P ++ G
Sbjct: 519 EEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTG 576
Query: 524 YTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQ 345
Y+ V+ HTA FA++ K+D+ T + ++ P G I L P+C+E F+
Sbjct: 577 YSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFE 635
Query: 344 EFPPLGRFAVRDMRQTVAVG-VIKAVN 267
++ +GRF +RD TVAVG V+K ++
Sbjct: 636 DYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 48.0 bits (109), Expect = 1e-06
Identities = 41/138 (29%), Positives = 62/138 (44%)
Frame = -2
Query: 689 AVPGDNVGFXVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 510
AV GD V + + V +LR G + + +N P + F A++ + G I +G T VL
Sbjct: 461 AVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL 519
Query: 509 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 330
H+ + K+ K + + S K I L PLC+ +E P L
Sbjct: 520 -----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPAL 571
Query: 329 GRFAVRDMRQTVAVGVIK 276
GRF +R TVA G++K
Sbjct: 572 GRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 33.1 bits (72), Expect = 0.042
Identities = 32/150 (21%), Positives = 60/150 (40%)
Frame = -2
Query: 728 VXSVEMHHEALQEAVPGDNVGFXVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 549
V +EM + L AV GDN G ++ + ++L+RG + P F A +L
Sbjct: 298 VTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILT 355
Query: 548 HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSK 369
+ T +D + + + +++ ++ T + K + GD +
Sbjct: 356 K--EEGGRRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIH 410
Query: 368 PLCVESFQEFPPLGRFAVRDMRQTVAVGVI 279
P+ +E Q RF VR+ TV ++
Sbjct: 411 PIVIEKGQ------RFTVREGGSTVGTALV 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.6
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +3
Query: 633 QFLDGHVLYVETYIVSRYSFLESFV 707
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 6.4
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +1
Query: 226 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 399
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 400 SPDLMDFGLTSVDLPVRRSTFSLIS 474
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.4
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +1
Query: 391 MAASPDLMDFGLTSVDLPVRRSTFS 465
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,915,004
Number of Sequences: 5004
Number of extensions: 58412
Number of successful extensions: 163
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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