Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_pT_I05
(641 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 2.3
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 27 2.3
SPBC30B4.08 |eri1||double-strand siRNA ribonuclease|Schizosaccha... 27 2.3
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 27 2.3
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 27 2.3
SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual 26 5.3
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 26 5.3
SPAC589.06c |||pho88 family protein|Schizosaccharomyces pombe|ch... 25 7.0
SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 25 7.0
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.3
SPAC22E12.01 ||SPAC890.09|triose phosphate transporter |Schizosa... 25 9.3
SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po... 25 9.3
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 25 9.3
>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 230
Score = 27.1 bits (57), Expect = 2.3
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = +1
Query: 238 LVVYKTLLIFFFLVLVAQDTSFVIVFAFKTIRVDITIFTAGNTVYSNGFF 387
L +++ LL+FFF +L SF F F ++ I F++ ++ FF
Sbjct: 110 LSLFRCLLLFFFFLLFFLSFSFSFSFLFFLSQIFIVYFSSFPILHFLFFF 159
>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
Mad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 27.1 bits (57), Expect = 2.3
Identities = 28/102 (27%), Positives = 44/102 (43%), Gaps = 4/102 (3%)
Frame = -1
Query: 527 LETHNRNTAQLIQEKRNQSLKLFVNTEMKTRMEALH-GASKTTTVLLRKKPLE*TVLPAV 351
L ++NRN L +EK + KL+ E + ++ L K T L K L LP
Sbjct: 260 LSSNNRNVEILKEEKNDLESKLYRFEEYRDKVATLELENEKIQTELNSWKSLITNELPTP 319
Query: 350 NMVMSTLMVL---NANTITKLVSCATRTRKKKIRRVL*TTKK 234
V + L+ L NAN ++ S ++ K + L +K
Sbjct: 320 EAVSNKLVFLQNTNANLGERVSSLESQLSNKPANQPLGANEK 361
>SPBC30B4.08 |eri1||double-strand siRNA
ribonuclease|Schizosaccharomyces pombe|chr 2|||Manual
Length = 313
Score = 27.1 bits (57), Expect = 2.3
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = -3
Query: 324 LKREYNYETGILCDKNKEEKDQKGFIDYQENKAVLPNGITIDLSA 190
LKR + + L +K K+E+ QK F ENK L + +D+ A
Sbjct: 46 LKRRWKFREKRLEEKRKQERYQK-FSTSNENKTCLRYLLIVDVEA 89
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 27.1 bits (57), Expect = 2.3
Identities = 19/74 (25%), Positives = 29/74 (39%)
Frame = -3
Query: 411 ENDDGSFKEETIGIDCITRGKYGYVDPDGLKREYNYETGILCDKNKEEKDQKGFIDYQEN 232
E D+ + K + I+ R G LK Y YE L +N K ++ +
Sbjct: 784 EADEEAMKNKIFKIEGTQRKILGIHSRRKLKNSYEYECSFLVGENIGMKSERWVPMMSSD 843
Query: 231 KAVLPNGITIDLSA 190
A LP G ++ A
Sbjct: 844 NAWLPRGELMETHA 857
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 27.1 bits (57), Expect = 2.3
Identities = 16/50 (32%), Positives = 22/50 (44%)
Frame = -1
Query: 467 KLFVNTEMKTRMEALHGASKTTTVLLRKKPLE*TVLPAVNMVMSTLMVLN 318
+LF N EM+ M L A KTT + K +P V + T+ N
Sbjct: 15 RLFSNKEMRILMLGLDAAGKTTILYKLKLNQSVVTIPTVGFNVETVTYKN 64
>SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual
Length = 566
Score = 25.8 bits (54), Expect = 5.3
Identities = 17/55 (30%), Positives = 26/55 (47%)
Frame = -1
Query: 539 SAPPLETHNRNTAQLIQEKRNQSLKLFVNTEMKTRMEALHGASKTTTVLLRKKPL 375
+AP E N LI E +NQ+L E + R++ L + TT ++K L
Sbjct: 14 TAPATEQENALNTALINELKNQNL-FESPAESEKRVKVLDELQQITTEFVKKVSL 67
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 25.8 bits (54), Expect = 5.3
Identities = 17/41 (41%), Positives = 26/41 (63%)
Frame = -3
Query: 351 KYGYVDPDGLKREYNYETGILCDKNKEEKDQKGFIDYQENK 229
+YG+ D D +KR+ ET L NKEEK++K D +++K
Sbjct: 162 RYGFSD-DIMKRKL--ETEKLEANNKEEKEEKEGKDDKDDK 199
>SPAC589.06c |||pho88 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 202
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 430 SILVFISVFTNNLSDWFLFS 489
SIL IS+FTNNL ++F+
Sbjct: 124 SILPLISLFTNNLVSIYIFN 143
>SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 624
Score = 25.4 bits (53), Expect = 7.0
Identities = 27/112 (24%), Positives = 43/112 (38%), Gaps = 3/112 (2%)
Frame = -3
Query: 534 APVRNTQQKYSPTNTREKKPVAQ---IIRKYRDENEDGSITWGFENDDGSFKEETIGIDC 364
AP N Q ++ TNT K P + I E+++ W + F + +D
Sbjct: 360 APYPNPYQNFTTTNTTVKNPFERFDTIDLVDGGEDDENIPIWPLLHPQ-RFVDVIFAVDA 418
Query: 363 ITRGKYGYVDPDGLKREYNYETGILCDKNKEEKDQKGFIDYQENKAVLPNGI 208
G+ PDG YE I + NK D +GF + ++ G+
Sbjct: 419 TYDDSNGW--PDGSSIVTTYERIITYNANK-SVDVRGFPYIPDEDTIISLGL 467
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 25.0 bits (52), Expect = 9.3
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = +3
Query: 171 SWFSYPWQRDLL*YHWVG 224
+W +YPWQ +L H G
Sbjct: 439 AWLTYPWQTQILRLHLDG 456
>SPAC22E12.01 ||SPAC890.09|triose phosphate transporter
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 374
Score = 25.0 bits (52), Expect = 9.3
Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 4/63 (6%)
Frame = +1
Query: 331 RVDITIFTAGNTVYSNGFFLKRTVVVFEAPCNASILVFI----SVFTNNLSDWFLFSCIS 498
R I G + + L+ + F C +SIL+F+ +F + DW L CI+
Sbjct: 126 RAGICALVTGLDIGLSNASLETITLSFYTMCRSSILIFVFFFSVIFRIEMFDWILL-CIT 184
Query: 499 WAV 507
+
Sbjct: 185 LVI 187
>SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 472
Score = 25.0 bits (52), Expect = 9.3
Identities = 20/71 (28%), Positives = 30/71 (42%), Gaps = 6/71 (8%)
Frame = +1
Query: 403 VVFEAPCNASILVFISVFTNNLSDWFLFSCISWAVFLLCVSNGGADWLLR------SCVL 564
V+FE CN S + N DW ++C +W F + +LLR + +
Sbjct: 335 VIFEGVCNFSAEITRFADRNFYDDW--WNCWTWDQFARTWNKPVHYFLLRHVYVPLNSFM 392
Query: 565 SDGLDFFFLFY 597
S L FF F+
Sbjct: 393 SKSLSTFFTFF 403
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 25.0 bits (52), Expect = 9.3
Identities = 18/90 (20%), Positives = 43/90 (47%), Gaps = 1/90 (1%)
Frame = -3
Query: 489 REKKPVAQIIRKYRDENEDGSITWGFENDDGSFKEETIGIDCITRGKYGYVDPDGLKREY 310
R+ + II + + +D N D ++T G+ + + ++D G+K Y
Sbjct: 1161 RKNSELCDIIEALKYQIQDQETEIISLNADNLDLKDTNGV--LEKNASDFIDFQGIKSRY 1218
Query: 309 NYETGILCDKNKEEKDQKGFIDYQ-ENKAV 223
++ L ++ ++E+ + G + + EN++V
Sbjct: 1219 EHKISDLLNQLQKERCKVGLLKQKTENRSV 1248
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,710,992
Number of Sequences: 5004
Number of extensions: 62045
Number of successful extensions: 174
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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