BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_pT_B05
(523 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 50 2e-07
SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 32 0.060
SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 28 0.73
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p... 26 3.0
SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr 3|||Ma... 26 3.9
SPBC1271.02 |stt3||oligosaccharyltransferase subunit Stt3|Schizo... 25 6.8
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.8
SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 25 9.0
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 50.0 bits (114), Expect = 2e-07
Identities = 35/110 (31%), Positives = 55/110 (50%), Gaps = 4/110 (3%)
Frame = -1
Query: 499 LDDRIRMVYTEAVILETLRISTVASMGIPHMALNDAKLG-NYIIPKGTFILLSLY-ELHH 326
LD +M YT AV+ E LR+ M +P+ + +Y +PK ++ +LY LH
Sbjct: 363 LDLMEKMTYTRAVVKECLRLRPPVLM-VPYRVKKAFPITPDYTVPKDAMVIPTLYGALHD 421
Query: 325 GPHWKDPETFRPERFLTKEGNILQD--EWLIPFGIGKRRCIGEGLARSEL 182
+ +PETF P+R+ G Q W++ FG G C+G+ A + L
Sbjct: 422 SKVYPEPETFNPDRW-APNGLAEQSPKNWMV-FGNGPHVCLGQRYAVNHL 469
>SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 495
Score = 31.9 bits (69), Expect = 0.060
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -1
Query: 241 IPFGIGKRRCIGEGLARSELFMFLTHILQKF 149
+PFG G+ RCIGE A +M L+ I+ KF
Sbjct: 433 LPFGAGRHRCIGEQFA----YMHLSTIISKF 459
>SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 449
Score = 28.3 bits (60), Expect = 0.73
Identities = 20/60 (33%), Positives = 30/60 (50%)
Frame = +3
Query: 126 SFLGILRWNF*RI*VRNMKSSERANPSPIQRRFPIPKGMSHSSCKMLPSLVRNRSGRNVS 305
S ILRW+F ++ +N SS +N SP ++ I K + +C LV N + R S
Sbjct: 124 SIFSILRWSFQKLGFQNQASSILSNRSP-SGQYVIRKNIEKLAC-----LVHNEAMRRCS 177
>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 595
Score = 26.2 bits (55), Expect = 3.0
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +3
Query: 174 NMKSSERANPSPIQRRFPIPKGMSHSSCKMLPSLVRN 284
N + E+ N P+Q P+P MS +S + S + N
Sbjct: 41 NRLAVEKPNIQPLQGHSPVPSFMSTASTNISSSKINN 77
>SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 628
Score = 25.8 bits (54), Expect = 3.9
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -2
Query: 99 MDFHCPLSNSGLFSNREKH 43
M FH P+SNSG + R H
Sbjct: 200 MQFHLPISNSGAATPRSVH 218
>SPBC1271.02 |stt3||oligosaccharyltransferase subunit
Stt3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 752
Score = 25.0 bits (52), Expect = 6.8
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = -1
Query: 442 ISTVASMGIPHMALNDAKLGNYIIPKGTFILLSLYELHH 326
I T+ASM +P + ++ G F LL L+ ++
Sbjct: 252 IGTLASMQVPFVGFQPVSTSEHMSALGVFGLLQLFAFYN 290
>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 386
Score = 25.0 bits (52), Expect = 6.8
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1
Query: 139 IPKNEPLPSTEPIDGLSLSAKQFRIIFEP 53
+P N +P TEP+D SLS Q R P
Sbjct: 190 LPSNS-IPKTEPLDTPSLSNSQERFSKSP 217
>SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 567
Score = 24.6 bits (51), Expect = 9.0
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +3
Query: 264 LPSLVRNRSGRNVSGSFQCGP*CSSYSDNKIKVPFG 371
L S R N S S P S +SD ++ VP+G
Sbjct: 207 LVSAGNGRETVNASASGAVNPTSSQWSDQRLGVPYG 242
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,259,709
Number of Sequences: 5004
Number of extensions: 50043
Number of successful extensions: 158
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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