Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_F_M05
(759 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 48 2e-06
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 35 0.011
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 35 0.014
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 34 0.025
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 30 0.41
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 28 1.3
SPAC823.10c |||mitochondrial carrier with solute carrier repeats... 26 6.7
SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|... 26 6.7
SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M... 26 6.7
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 25 8.9
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 8.9
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 47.6 bits (108), Expect = 2e-06
Identities = 35/106 (33%), Positives = 52/106 (49%), Gaps = 7/106 (6%)
Frame = +2
Query: 458 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 634
+ ++ F D+DQ G ITS EL ++ + GQ+ + MI D D NG I+ EF
Sbjct: 13 EFREAFSLFDRDQDGNITSNEL-GVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFL 71
Query: 635 ----DKLYTYVN--QWLAVFKTYDTDQSGFIXEGELSKALTQMGFR 754
K+ N + FK +D D +G+I EL+ LT +G R
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 117
Score = 40.3 bits (90), Expect = 3e-04
Identities = 22/62 (35%), Positives = 35/62 (56%)
Frame = +2
Query: 458 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 637
+V++ F+ DKD +G+IT EL L + G+ S+ MI D D +G IN +EF
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSL-GERLSQEEVADMIREADTDGDGVINYEEFS 144
Query: 638 KL 643
++
Sbjct: 145 RV 146
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.1 bits (77), Expect = 0.011
Identities = 28/102 (27%), Positives = 45/102 (44%), Gaps = 3/102 (2%)
Frame = +2
Query: 458 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 637
++++ F +DKD G I ++++ L + Q SE + N M + N L
Sbjct: 49 ELKEAFALLDKDGDGNIGREDVKTMLTSLN-QDASEDSINHMFESINPPINLAAFLTAMG 107
Query: 638 KLYTYV---NQWLAVFKTYDTDQSGFIXEGELSKALTQMGFR 754
+ + N L F T+D QSG I + AL+ MG R
Sbjct: 108 SMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDR 149
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 34.7 bits (76), Expect = 0.014
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 9/98 (9%)
Frame = +2
Query: 458 QVQQWFRAVDKD-QSGFITSTELRSALVNAQGQTFSETAC--NLMIGMFDKDRNGQINLD 628
++QQW++ KD SG + +E + + Q F + + + +FD D+NG I+
Sbjct: 26 ELQQWYKGFFKDCPSGHLNKSEFQK--IYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFK 83
Query: 629 EFDKLYTYV------NQWLAVFKTYDTDQSGFIXEGEL 724
EF + ++ + F+ YD D +G I E+
Sbjct: 84 EFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEM 121
Score = 27.1 bits (57), Expect = 2.9
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 563 ETACNLMIGMFDKDRNGQINLDEF 634
E N + M DK+++GQ+ L+EF
Sbjct: 146 EKRVNKIFNMMDKNKDGQLTLEEF 169
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 33.9 bits (74), Expect = 0.025
Identities = 28/103 (27%), Positives = 45/103 (43%), Gaps = 7/103 (6%)
Frame = +2
Query: 458 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 634
++++ F +D +QSG I E S A S + + + D+D G ++ EF
Sbjct: 25 RIRKRFIKIDANQSGSIDRNEFLSIPSVA-----SNPLASRLFSVVDEDGGGDVDFQEFI 79
Query: 635 DKLYTYV------NQWLAVFKTYDTDQSGFIXEGELSKALTQM 745
+ L + + FK YD D+ G+I GEL L M
Sbjct: 80 NSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMM 122
Score = 31.5 bits (68), Expect = 0.13
Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 4/66 (6%)
Frame = +2
Query: 473 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGM----FDKDRNGQINLDEFDK 640
F+ D D+ G+I++ EL L G E ++ DKDR+G+I+ +EF
Sbjct: 99 FKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKD 158
Query: 641 LYTYVN 658
+ + N
Sbjct: 159 IVSGSN 164
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 29.9 bits (64), Expect = 0.41
Identities = 15/54 (27%), Positives = 26/54 (48%)
Frame = +2
Query: 473 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 634
FR DKD SG+I + + + + G+ S+ LM+ D +G + +F
Sbjct: 84 FRVFDKDNSGYIETAKF-ADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/54 (33%), Positives = 27/54 (50%)
Frame = +2
Query: 473 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 634
F+ DKD +G I ELR L + + +E L+ G+ KD G +N +F
Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD--GMVNYHDF 134
>SPAC823.10c |||mitochondrial carrier with solute carrier
repeats|Schizosaccharomyces pombe|chr 1|||Manual
Length = 296
Score = 25.8 bits (54), Expect = 6.7
Identities = 13/54 (24%), Positives = 28/54 (51%), Gaps = 4/54 (7%)
Frame = +1
Query: 382 WNATRP--IRNWTWTVPFHW--SWWYYYAPSTAVVPSCR*RSIWIHYFDRTKIG 531
W T P +R+ T + + + +W ++AP + + S +++W+ F R +G
Sbjct: 68 WKGTLPSILRSTTGSSCYFYFLNWLRHFAPQSKNIASSHIQNLWMGGFARAAVG 121
>SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 539
Score = 25.8 bits (54), Expect = 6.7
Identities = 20/66 (30%), Positives = 29/66 (43%)
Frame = +2
Query: 365 GQAAYGGMPPGQLEIGHGPYPSIGVGGTITPQVQQWFRAVDKDQSGFITSTELRSALVNA 544
G+ A+GG L+ GP + V G I + W + SG + +TEL S V +
Sbjct: 304 GRQAWGGFSREALK-NWGPLCRLAVPGVIMICSEYWAFELVTFASGVLGTTELASMSVLS 362
Query: 545 QGQTFS 562
T S
Sbjct: 363 TTSTLS 368
>SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1115
Score = 25.8 bits (54), Expect = 6.7
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -3
Query: 604 VFVEHTNHQIASSFGKSLT 548
V+ H N QI SSFG SLT
Sbjct: 33 VWTGHKNGQIKSSFGPSLT 51
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 25.4 bits (53), Expect = 8.9
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = +2
Query: 335 LQMAYNSGYPGQAAYGG 385
+Q Y+ GYPG YGG
Sbjct: 61 MQNKYSEGYPGARYYGG 77
>SPBC30D10.10c |tor1||phosphatidylinositol kinase
Tor1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2335
Score = 25.4 bits (53), Expect = 8.9
Identities = 8/22 (36%), Positives = 16/22 (72%)
Frame = -2
Query: 605 GLCRTYQSSDCKQFRKKFDLER 540
GLC T ++D + F+++ ++ER
Sbjct: 2002 GLCNTLLTTDSETFKRRLNIER 2023
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 8.9
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -2
Query: 431 WKGTVHVQFLIGLVAFHRKLPVLDIRNCRPFVVS 330
W G + L+GL+ + ++DI++ RP + S
Sbjct: 297 WTGNPKDKELVGLIPLLEFIAIMDIKDVRPVLKS 330
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,981,391
Number of Sequences: 5004
Number of extensions: 60142
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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