Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_F_L15
(732 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 184 1e-47
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 108 9e-25
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 107 2e-24
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 92 6e-20
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 90 3e-19
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 90 3e-19
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 77 3e-15
SPBPB8B6.04c |grt1|SPAPB8B6.04c, SPAPB8B6.04c|transcription fact... 30 0.30
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 29 0.68
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 27 3.6
SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos... 26 6.4
SPAC343.17c |||WD repeat protein, human WDR70 family|Schizosacch... 26 6.4
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 184 bits (448), Expect = 1e-47
Identities = 89/181 (49%), Positives = 124/181 (68%), Gaps = 1/181 (0%)
Frame = +2
Query: 191 GLTSVALRGTDAAVVAAQRKVPDRLLDPASVTHLFALTDRIGCVMTGMIADSRSQVQRAR 370
G+TSV + G + A V +Q+KVPD+L+D ++V H+F +T IGCVMTG IAD+R+QV RAR
Sbjct: 34 GITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFPITKGIGCVMTGSIADARAQVSRAR 93
Query: 371 YEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYKTD 550
EAA ++YK G +P +L +R+A+I+QV TQ A MRPLG +M L+A DDE GP ++K D
Sbjct: 94 SEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQRAAMRPLGVAMTLVAVDDEIGPSLFKLD 153
Query: 551 PSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLST-DDAIQLAISCLSQVLSVDFKSSX 727
P+G+Y YKA +AG K + +LEK+ KK S D ++ I L LS DFKS+
Sbjct: 154 PAGFYIGYKATSAGPKQTETINWLEKRFKKDGIPSNLTDTVETGIQALMSSLSTDFKSTE 213
Query: 728 I 730
+
Sbjct: 214 L 214
Score = 62.5 bits (145), Expect = 6e-11
Identities = 28/50 (56%), Positives = 33/50 (66%)
Frame = +1
Query: 106 GFDRHITIFSPEGRLYQVEYALKAINQGWVDICGITRYRCSCSGSSTQGP 255
GFDR IT+FSPEGRLYQVEYA KA N + G+T C+C S + P
Sbjct: 6 GFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVP 55
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 108 bits (259), Expect = 9e-25
Identities = 66/182 (36%), Positives = 96/182 (52%), Gaps = 2/182 (1%)
Frame = +2
Query: 191 GLTSVALRGTDAAVVAAQRKVPDRLLDPASVTHLFALTDRIGCVMTGMIADSRSQVQRAR 370
G+TSV ++ TD V+A ++K L AS+ + A+T IG V +GM D R V ++R
Sbjct: 31 GVTSVGIKATDGVVLATEKKPTSELAIGASLEKVCAITPDIGMVYSGMGPDFRVLVDKSR 90
Query: 371 YEAANWQYK--YGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYK 544
+ A YK Y P IL + IA + Q TQ+ +RP G S+ L+A DE GP +Y+
Sbjct: 91 -KVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGGVRPFGVSL-LVAGMDEKGPSLYQ 148
Query: 545 TDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDDAIQLAISCLSQVLSVDFKSS 724
DPSG Y ++KA A G + +LEK+ +L DDA+ AI L + +
Sbjct: 149 VDPSGTYFAWKATAIGKSSTAAKTFLEKRY--NDELELDDAVHTAILALKETFEGELTED 206
Query: 725 XI 730
I
Sbjct: 207 NI 208
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +1
Query: 121 ITIFSPEGRLYQVEYALKAINQG 189
+T FSP G+L Q+EYAL A+N G
Sbjct: 9 LTTFSPNGKLVQIEYALNAVNAG 31
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 107 bits (256), Expect = 2e-24
Identities = 57/174 (32%), Positives = 96/174 (55%), Gaps = 1/174 (0%)
Frame = +2
Query: 185 RGGLTSVALRGTDAAVVAAQRKVPDRLLDPASVTHLFALTDRIGCVMTGMIADSRSQVQR 364
R G T++ALRG + V+ +RK +L + ++ + + + + G+ AD+R + +
Sbjct: 28 RRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQKIAMVDNHVCLAFAGLNADARILIDK 87
Query: 365 ARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYD-DEAGPCVY 541
AR EA N + + + L R +A + Q YTQ+ +RP G S ++ +D + P VY
Sbjct: 88 ARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFDVGDNTPRVY 147
Query: 542 KTDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDDAIQLAISCLSQVL 703
+T+P+G Y ++KA A G + YLEK K+ LS D+ I LA+S L +V+
Sbjct: 148 QTEPAGIYNAWKATAIGRASKAAREYLEKNWKE--GLSRDETIHLAVSSLLEVV 199
Score = 41.1 bits (92), Expect = 2e-04
Identities = 15/29 (51%), Positives = 24/29 (82%)
Frame = +1
Query: 103 AGFDRHITIFSPEGRLYQVEYALKAINQG 189
+G+DR +++FSP+GRL QVEY +A+ +G
Sbjct: 2 SGYDRALSVFSPDGRLLQVEYGQEAVRRG 30
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 92.3 bits (219), Expect = 6e-20
Identities = 53/165 (32%), Positives = 82/165 (49%)
Frame = +2
Query: 197 TSVALRGTDAAVVAAQRKVPDRLLDPASVTHLFALTDRIGCVMTGMIADSRSQVQRARYE 376
T + ++ D ++A ++ V +LL P + ++ IG TG I D + V+RAR E
Sbjct: 35 TCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFIPDGQHIVKRARDE 94
Query: 377 AANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYKTDPS 556
A +W+ YGS IP ++ R+ + Q++T + +RP G + YD E GP +Y +P+
Sbjct: 95 ATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSE-GPHLYMVEPN 153
Query: 557 GYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDDAIQLAISCL 691
G Y Y AAG LEK S L DA++ A L
Sbjct: 154 GVYWGYNGAAAGKGRQVARNELEK--LNFSSLKMKDAVKEAARIL 196
Score = 32.7 bits (71), Expect = 0.056
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +1
Query: 106 GFDRHITIFSPEGRLYQVEYALKAI 180
G+D + FSP+GRL+Q EYA KA+
Sbjct: 7 GYDLGL-FFSPDGRLFQAEYAYKAV 30
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 89.8 bits (213), Expect = 3e-19
Identities = 48/162 (29%), Positives = 87/162 (53%), Gaps = 1/162 (0%)
Frame = +2
Query: 221 DAAVVAAQRKVPDRLLDPA-SVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYK 397
D V+AA++KV +LL+ S L+ + D + C + G+ AD+ + AR +
Sbjct: 41 DGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQT 100
Query: 398 YGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYK 577
+ E+P L RR+ D+ Q YTQ +RP G S + +D G +++++PSG Y S++
Sbjct: 101 FNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQ 160
Query: 578 AVAAGAKTVDGNAYLEKKLKKRSDLSTDDAIQLAISCLSQVL 703
A + G + + + ++ K D++ D+A +A+ LS+ L
Sbjct: 161 ANSIGGNSTSVQSLMRQEYK--DDINLDEASAMAVKFLSKTL 200
Score = 48.0 bits (109), Expect = 1e-06
Identities = 22/32 (68%), Positives = 24/32 (75%)
Frame = +1
Query: 100 SAGFDRHITIFSPEGRLYQVEYALKAINQGWV 195
S +D TIFSPEGRLYQVEYAL+AIN V
Sbjct: 2 SRSYDSRTTIFSPEGRLYQVEYALEAINHAGV 33
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 89.8 bits (213), Expect = 3e-19
Identities = 52/186 (27%), Positives = 98/186 (52%), Gaps = 6/186 (3%)
Frame = +2
Query: 191 GLTSVALRGTDAAVVAAQRKVPDRLLDPASVTHLFALTDRIGCVMTGMIADSRSQVQRAR 370
G T++ ++ DA V+ ++++ L++ SV LF + IGC ++G+ AD+R+ ++ AR
Sbjct: 34 GSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEHAR 93
Query: 371 YEAANWQYKYGSEIPVHILCRRIADISQVYTQNAE------MRPLGCSMMLIAYDDEAGP 532
+ N ++ Y + + I D++ + + + RP G + +LIA DE GP
Sbjct: 94 VQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVA-LLIAGIDEHGP 152
Query: 533 CVYKTDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDDAIQLAISCLSQVLSVD 712
+Y ++PSG Y Y+A A G+ + + L K+ K D++ ++A L + L QV+
Sbjct: 153 QLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHK--DMTLEEAEVLILKVLRQVMEEK 210
Query: 713 FKSSXI 730
S +
Sbjct: 211 LDSKNV 216
Score = 41.9 bits (94), Expect = 9e-05
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +1
Query: 109 FDRHITIFSPEGRLYQVEYALKAINQG 189
+DR + FSPEGRL+QVEYA++AI G
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKLG 34
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 77.0 bits (181), Expect = 3e-15
Identities = 49/174 (28%), Positives = 82/174 (47%)
Frame = +2
Query: 191 GLTSVALRGTDAAVVAAQRKVPDRLLDPASVTHLFALTDRIGCVMTGMIADSRSQVQRAR 370
G +V L AV+ A ++ + L + L + D IG + G+ D+R +
Sbjct: 32 GSATVGLVSKTHAVLVALKRNAEEL--SSYQKKLIRIDDHIGIAIAGLAPDARVLSNYMK 89
Query: 371 YEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCVYKTD 550
EA + + + IPV L ++A+ +Q+ TQ RP G ++I Y DE+GP + +
Sbjct: 90 QEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGY-DESGPHLLEFQ 148
Query: 551 PSGYYCSYKAVAAGAKTVDGNAYLEKKLKKRSDLSTDDAIQLAISCLSQVLSVD 712
PSG Y + G+++ Y+E+ L D S ++ I A+ L LS D
Sbjct: 149 PSGLVLEYLGTSMGSRSQSARTYIERNLDTFPDSSREELILSALRALRDTLSKD 202
Score = 39.9 bits (89), Expect = 4e-04
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +1
Query: 109 FDRHITIFSPEGRLYQVEYALKAINQG 189
+D T +SP+GRL+QVEYAL+AI QG
Sbjct: 6 YDGDATTWSPQGRLHQVEYALEAIKQG 32
>SPBPB8B6.04c |grt1|SPAPB8B6.04c, SPAPB8B6.04c|transcription factor
Grt1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 648
Score = 30.3 bits (65), Expect = 0.30
Identities = 15/50 (30%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +1
Query: 349 VSSTTSSL*GCQLAVQVW--VRDTCAYLMQTHS*YLSSLHPKCRDETSGL 492
++S ++ + G QLA ++W +RDTC++L + Y+ S+ +D L
Sbjct: 520 IASNSAKIQG-QLATEIWAVLRDTCSFLQGSSGPYVGSVSTIAKDALESL 568
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 29.1 bits (62), Expect = 0.68
Identities = 15/62 (24%), Positives = 25/62 (40%)
Frame = +2
Query: 329 GMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADISQVYTQNAEMRPLGCSMMLI 508
GM +R+ Q +E +W Y + V LCR + ++ N + + LI
Sbjct: 606 GMCPYTRAVSQHVEWENDSWMYVLNVSLQVAKLCRHVGNVFMELNTNKLANAINYLISLI 665
Query: 509 AY 514
Y
Sbjct: 666 LY 667
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 26.6 bits (56), Expect = 3.6
Identities = 21/91 (23%), Positives = 39/91 (42%), Gaps = 1/91 (1%)
Frame = +2
Query: 275 ASVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYKYGSEIPVHILCRRIADIS- 451
A++ +L+ +I CV AD Q QR + + + G P HI I + S
Sbjct: 508 ANLLNLYPDLQKIPCVFAATKADLDRQQQRYPVQPDEFTKQLGLPSPTHISTAAIWNTSK 567
Query: 452 QVYTQNAEMRPLGCSMMLIAYDDEAGPCVYK 544
+ + Q AE S ++ ++++ Y+
Sbjct: 568 EFFIQLAESAQYPASSIIRIPEEDSNKTNYQ 598
>SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 584
Score = 25.8 bits (54), Expect = 6.4
Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Frame = +2
Query: 137 LREDCIKLNMH*KQ*TRGGLTSVALRGTDAAVVAAQRKVPD--RLLDP 274
+ EDC K+N+H + G L A G+ ++ K D + +DP
Sbjct: 328 ITEDCNKINVHPRHPYAGNLVFTAFSGSHQDAISKGLKAYDERKAVDP 375
>SPAC343.17c |||WD repeat protein, human WDR70
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 576
Score = 25.8 bits (54), Expect = 6.4
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2
Query: 383 WQPHSELVVLETGYQQSSR 327
WQP S + L TGY ++R
Sbjct: 205 WQPDSSQIFLTTGYDSTAR 223
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,805,295
Number of Sequences: 5004
Number of extensions: 53626
Number of successful extensions: 166
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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