Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_F_K19
(798 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 4e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 8e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 2e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 1e-29
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.4
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.1
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 27 4.1
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.1
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.4
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.2
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 9.5
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 235 bits (576), Expect = 4e-63
Identities = 101/132 (76%), Positives = 117/132 (88%)
Frame = +1
Query: 109 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 288
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 289 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 468
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 469 RKEAESCDCLQG 504
R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132
Score = 124 bits (300), Expect = 1e-29
Identities = 60/101 (59%), Positives = 70/101 (69%)
Frame = +2
Query: 494 ASRGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLS 673
A +GFQ KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188
Query: 674 VHQLXENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLV 796
+HQL EN+DET+CIDNEAL I TLK+ +P+Y DLNHLV
Sbjct: 189 MHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLV 229
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 8e-33
Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%)
Frame = +1
Query: 109 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 282
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 283 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 462
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 463 VVRKEAESCDCLQG 504
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 74.5 bits (175), Expect = 2e-14
Identities = 32/76 (42%), Positives = 49/76 (64%)
Frame = +2
Query: 569 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLXENTDETYCIDNEALYDICFR 748
++ EY + +SV P+P+VS +VVEPYN+ L+ H + D T+ +DNE+ YDIC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Query: 749 TLKLSTPTYGDLNHLV 796
L + P+Y +LN L+
Sbjct: 216 NLDIERPSYENLNRLI 231
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (314), Expect = 2e-31
Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%)
Frame = +1
Query: 109 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 270
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 271 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 450
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 451 SVLDVVRKEAESCDCLQG 504
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 81.0 bits (191), Expect = 2e-16
Identities = 35/76 (46%), Positives = 51/76 (67%)
Frame = +2
Query: 569 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLXENTDETYCIDNEALYDICFR 748
++ EY + +SV P+P+VS +VVEPYN+ L+ H +N+D T+ +DNEA YDIC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Query: 749 TLKLSTPTYGDLNHLV 796
L + PTY +LN L+
Sbjct: 220 NLDIERPTYENLNRLI 235
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 124 bits (299), Expect = 1e-29
Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%)
Frame = +1
Query: 112 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 291
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 292 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 465
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 466 VRKEAESCDCLQG 504
+ +EA+ D L+G
Sbjct: 122 IDREADGSDSLEG 134
Score = 63.7 bits (148), Expect = 3e-11
Identities = 32/77 (41%), Positives = 44/77 (57%), Gaps = 1/77 (1%)
Frame = +2
Query: 569 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLXENTDETYCIDNEALYDICF 745
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215
Query: 746 RTLKLSTPTYGDLNHLV 796
L PT+ N LV
Sbjct: 216 DRLHTQNPTFHQQNQLV 232
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.4
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 412 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 510
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.1
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 148 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 249
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 26.6 bits (56), Expect = 4.1
Identities = 16/46 (34%), Positives = 28/46 (60%)
Frame = -1
Query: 261 VIYIDALQLQVRVPMVGTSGVDAVLVGDDLPELSSDLVAALTSLDM 124
++Y+DA L +++ + SG A V DLP L +D V++L S+ +
Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.1
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 343 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 444
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 5.4
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 485 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 348
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 7.2
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 364 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 495
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 9.5
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 401 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 306
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,007,766
Number of Sequences: 5004
Number of extensions: 57910
Number of successful extensions: 188
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -