BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_F_I19
(679 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC645.03c |isa1||iron-sulfur protein Isa1|Schizosaccharomyces ... 125 7e-30
SPBC3B9.17 |isa2||iron-sulfur protein Isa2|Schizosaccharomyces p... 48 2e-06
SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 27 2.5
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 25 7.6
SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit Git... 25 7.6
>SPCC645.03c |isa1||iron-sulfur protein Isa1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 190
Score = 125 bits (301), Expect = 7e-30
Identities = 53/114 (46%), Positives = 83/114 (72%)
Frame = +1
Query: 127 RILPSRAALVLTPSAVKKVKEIMQKEEAKGYVGLKVGVRQRGCNGLSYTLDYAKTKEKLD 306
R +P + + LTP AV+ +K++ KG + L++GV+Q+GC G +Y+L+Y + +K D
Sbjct: 75 RFMPRKNVIKLTPLAVEHLKKMQSSASMKGKM-LRIGVKQKGCAGQAYSLEYIEKPDKFD 133
Query: 307 EEVKQDGVTIIIDKKAQLTLLGTEMDYVEDKLSAEFVFNNPNIKGTCGCGESFS 468
E VKQDG++II+ ++A L ++G+ MDY +D L + F+F+NPN+K TCGCGESFS
Sbjct: 134 EIVKQDGISIIVARRALLQIIGSVMDYRDDDLQSRFIFSNPNVKSTCGCGESFS 187
>SPBC3B9.17 |isa2||iron-sulfur protein Isa2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 205
Score = 47.6 bits (108), Expect = 2e-06
Identities = 27/108 (25%), Positives = 53/108 (49%), Gaps = 1/108 (0%)
Frame = +1
Query: 151 LVLTPSAVKKVKEIMQKEEAKGYVGLKVGVRQRGCNGLSYTLDYAKTKEKLDEEVKQDGV 330
L + SA K++++I +++ + V L+V V GC+G + D +
Sbjct: 98 LYVKDSAKKQLEKIAERKPEENSV-LRVTVDGGGCHGYQVSFRMDNKIGNADTVFVRGKA 156
Query: 331 TIIIDKKAQLTLLGTEMDYVEDKLSAEF-VFNNPNIKGTCGCGESFSI 471
++ D + + G+E++Y + + + F + NNP K +CGC SF +
Sbjct: 157 RVVADNISLPLISGSEIEYTNELIGSSFQLLNNPRAKTSCGCNVSFDV 204
>SPAC22A12.11 |dak1||dihydroxyacetone kinase
Dak1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 27.1 bits (57), Expect = 2.5
Identities = 15/60 (25%), Positives = 29/60 (48%)
Frame = +1
Query: 178 KVKEIMQKEEAKGYVGLKVGVRQRGCNGLSYTLDYAKTKEKLDEEVKQDGVTIIIDKKAQ 357
KVKE + KE V + G NGL + + +T +++ E ++ + +ID+ +
Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNGLGFGITLLRTTDRVKVEGEEYSLVDLIDQPVE 345
>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 809
Score = 25.4 bits (53), Expect = 7.6
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = +1
Query: 85 TKIIASATVRAVKKRILPSRAALVLTPSAVKKVKEIMQKEEAKG 216
+K++ + +AVKK ++PS + PS K + + + +KG
Sbjct: 524 SKVVVPSNAKAVKKSVVPSSP---VVPSPRKATNKSLSMDVSKG 564
>SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit
Git5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 305
Score = 25.4 bits (53), Expect = 7.6
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = +1
Query: 349 KAQLTLLGTEMDYVEDKLSAEFVFNNPNIKGTCGC 453
KA L G EMD V S +F+ +NPN+ T GC
Sbjct: 138 KAISVLKGHEMDIV----SLDFLPSNPNLFVTGGC 168
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,461,765
Number of Sequences: 5004
Number of extensions: 45861
Number of successful extensions: 108
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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