Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_F_G23
(787 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 152 5e-38
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 44 3e-05
SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces pomb... 29 0.76
SPAC824.08 |gda1|gdp1|guanosine-diphosphatase Gda1|Schizosacchar... 29 1.00
SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|ch... 28 1.3
SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac... 27 4.0
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 7.0
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 26 7.0
>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 538
Score = 152 bits (369), Expect = 5e-38
Identities = 74/134 (55%), Positives = 102/134 (76%), Gaps = 6/134 (4%)
Frame = +2
Query: 404 SSCRKVKEEVSKLLALKAQLV--TEDAGPQK---FTLKTPKGTRDYNPQQMTIRNNVLDK 568
+S + +EV+KLL LK + +E +G +K FTLKTPKGT+D+ + + +R ++
Sbjct: 27 ASKEDIDKEVAKLLQLKNLKLGGSEVSGKKKDTSFTLKTPKGTKDWCDKDVVLREHIFST 86
Query: 569 IITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYL 748
+ +F+RHG +DTPVFELK++L+GKYGEDSKLIYDLKDQGGE+ SLRYDLTVP AR+L
Sbjct: 87 VTEIFKRHGGVTLDTPVFELKEILSGKYGEDSKLIYDLKDQGGELCSLRYDLTVPFARWL 146
Query: 749 XMN-KITTLKRYHI 787
MN K+T++KR+HI
Sbjct: 147 AMNPKVTSIKRFHI 160
Score = 28.7 bits (61), Expect = 1.00
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +1
Query: 148 MADSQEDLLLKIQEQGDLVRKLKAAKDSNE 237
MADS D+ +I QG+LVR LK+ S E
Sbjct: 1 MADSLVDINERITSQGNLVRSLKSQGASKE 30
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 43.6 bits (98), Expect = 3e-05
Identities = 26/77 (33%), Positives = 41/77 (53%)
Frame = +2
Query: 557 VLDKIITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPL 736
V D ++ VFRRHGA+ ++ + K + +Y +D + L D+ G +L L YD +P
Sbjct: 990 VRDHVVKVFRRHGAKERESHILFPK---SSQYDKDQASV-SLLDKNGTLLQLPYDTVLPY 1045
Query: 737 ARYLXMNKITTLKRYHI 787
AR + N + K Y I
Sbjct: 1046 ARNVARNAVEEEKTYLI 1062
>SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1136
Score = 29.1 bits (62), Expect = 0.76
Identities = 18/78 (23%), Positives = 38/78 (48%), Gaps = 1/78 (1%)
Frame = +2
Query: 545 IRNNVLDKIITVFR-RHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYD 721
++N VL I++ + ++ +E K +L ++ +KL+ L G EILS+ +D
Sbjct: 466 LKNAVLSSIVSTIQIEPNSDLTGYDYYEYKRLLYNEWERFAKLVAYLDHFGDEILSINFD 525
Query: 722 LTVPLARYLXMNKITTLK 775
+ + NK+ ++
Sbjct: 526 PSNAVTYINYANKVAFIR 543
>SPAC824.08 |gda1|gdp1|guanosine-diphosphatase
Gda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 556
Score = 28.7 bits (61), Expect = 1.00
Identities = 18/46 (39%), Positives = 24/46 (52%)
Frame = -2
Query: 744 YRAKGTVKSYLNERISPPWSFKSYINFESSPYFPVSTSFSSKTGVS 607
YR++G V LN P + + F P FPV+TS +KT VS
Sbjct: 57 YRSEGEVVDLLN----PKDELEEPL-FSEEPLFPVTTSIPTKTAVS 97
>SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 404
Score = 28.3 bits (60), Expect = 1.3
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = -1
Query: 586 PKNCNNFIQYIITYCHLLWVVVSCSLWCFKSKL 488
P+N F+ II Y + + ++ CSLW + +KL
Sbjct: 318 PQNLQPFVFMIIQYLYSITTMLPCSLW-YNNKL 349
>SPAC23C4.02 |crn1||actin binding protein, coronin
Crn1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 26.6 bits (56), Expect = 4.0
Identities = 17/71 (23%), Positives = 32/71 (45%)
Frame = +2
Query: 572 ITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLX 751
+ +FR H A +DT D + G+DSK++ + ++ Y+ P+A
Sbjct: 74 VNLFRGHTAAVLDTDWNPFHDQVLASGGDDSKIMIWKVPEDYTVME-PYEDVHPIAELKG 132
Query: 752 MNKITTLKRYH 784
++ L +YH
Sbjct: 133 HSRKVGLVQYH 143
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.8 bits (54), Expect = 7.0
Identities = 17/55 (30%), Positives = 31/55 (56%), Gaps = 2/55 (3%)
Frame = +2
Query: 515 TRDYNPQQMTIRNNVLDKIITVFRRHGAECIDTPVFELKDV--LTGKYGEDSKLI 673
TRDY+ +Q+ R NV+ +++ F + A+ ++ P +K + L G Y E L+
Sbjct: 2511 TRDYHIKQVNNRPNVISTLVSSFVKCAAK-LELPPHLVKYLGKLYGVYHESVSLL 2564
>SPAPB8E5.07c |||ribosome biogenesis protein
Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1163
Score = 25.8 bits (54), Expect = 7.0
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -2
Query: 267 IFITRPFLVPLIGILRCF*FSNK 199
+FI+ PFLV LI L F S+K
Sbjct: 857 VFISEPFLVQLISTLNLFITSSK 879
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,240,408
Number of Sequences: 5004
Number of extensions: 69334
Number of successful extensions: 166
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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