Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P04_F_F21
(772 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 222 4e-59
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 103 3e-23
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 30 0.42
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 2.2
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 3.0
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 5.2
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 5.2
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 26 6.9
SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces pombe... 25 9.1
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 25 9.1
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 9.1
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 25 9.1
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 25 9.1
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 222 bits (543), Expect = 4e-59
Identities = 103/205 (50%), Positives = 138/205 (67%), Gaps = 1/205 (0%)
Frame = +2
Query: 161 IKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 340
++FL+++KPF LPEIA PERK+ F++K+LWT +TL IFLV Q+PL+GI+SSDS+DP
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63
Query: 341 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFGM 517
W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV + DR ++ QK +
Sbjct: 64 LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123
Query: 518 VITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAXXXXXXXXXXXXXXXXXXXXXXX 697
+I GQA YV+TGMYG P ++GAG+CLL+I+QL A
Sbjct: 124 IIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAASLIVLLLDELLQKGYGLGSGISL 183
Query: 698 XXATNICETIVWKAFSPXTVNTGRG 772
AT CE I WKAFSP T + G
Sbjct: 184 FIATINCENIFWKAFSPTTYHIANG 208
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 103 bits (247), Expect = 3e-23
Identities = 53/157 (33%), Positives = 91/157 (57%), Gaps = 1/157 (0%)
Frame = +2
Query: 158 GIKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 337
G +F+ IKP S+LPE+ P+ ++ EK+ W A + ++ + IP++G +D+ DP
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62
Query: 338 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFG 514
RV+ S+ LM G++PI S ++Q+LA K I V + DR LF AQK+
Sbjct: 63 INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKVVS 122
Query: 515 MVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFV 625
++ + A+ YV +G YG S++G +++I+Q+F+
Sbjct: 123 ALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFL 159
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.9 bits (64), Expect = 0.42
Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Frame = -2
Query: 507 NFCAPLNKALSFGVSPTS-MILAPASNCMMSPDVTIGEIPSSINV---PLFDARITRIQ* 340
+ CA +NKAL G+ TS A C PD I + S V PL + RI ++
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160
Query: 339 KGSALSD 319
G L D
Sbjct: 161 SGRVLVD 167
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 2.2
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -1
Query: 634 KTGHKQLYDNEEAHSGTNFTGFAIHSSHYINN-SLTNSNHHT 512
+T +Y + E H+G G +HSS NN S T+SN T
Sbjct: 144 QTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 3.0
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +2
Query: 161 IKFLEVI-KPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMS 319
I+FL+ KP+ S + R++ + K WT + I L+ PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 26.2 bits (55), Expect = 5.2
Identities = 17/66 (25%), Positives = 30/66 (45%), Gaps = 3/66 (4%)
Frame = -1
Query: 697 QRDTRTQSISFXEQ---FIEQQHNKTGHKQLYDNEEAHSGTNFTGFAIHSSHYINNSLTN 527
Q+ +S S + F E Q++ TG+K + A +G + G + +S +
Sbjct: 1700 QKSPSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIV 1759
Query: 526 SNHHTK 509
SNH T+
Sbjct: 1760 SNHQTE 1765
>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 5.2
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -2
Query: 387 SINVPLFDARITRIQ*KGSALSDDIIPNKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAI 208
S+ + LFD I+ + S +SD IP K +++ N + T+S N + +S
Sbjct: 273 SLLLGLFDKSISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKS 332
Query: 207 S 205
S
Sbjct: 333 S 333
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 6.9
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -2
Query: 210 ISGNILQNGLITSKNFIPILIYC 142
+ G L +GL+ K+F+P L C
Sbjct: 83 VQGEDLDDGLLVDKHFLPTLFKC 105
>SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 365
Score = 25.4 bits (53), Expect = 9.1
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = +2
Query: 719 ETIVWKAFSPXTVNTGRG 772
E +VW+ F TV TG G
Sbjct: 273 EDVVWETFDSQTVETGNG 290
>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 404
Score = 25.4 bits (53), Expect = 9.1
Identities = 6/15 (40%), Positives = 12/15 (80%)
Frame = +1
Query: 382 DGAWYLTYCNIRTHH 426
+G+W+ C+I++HH
Sbjct: 155 EGSWFCVTCSIKSHH 169
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 25.4 bits (53), Expect = 9.1
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -1
Query: 640 HNKTGHKQLYDNEEAHSGTNFTGFAIHSSHYINNSLTNSNH 518
+NK HK+L DNE SGT + + + + N T H
Sbjct: 515 NNKNHHKELEDNE---SGTRISPSTLGDTSFPNGIFTLLTH 552
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 25.4 bits (53), Expect = 9.1
Identities = 15/56 (26%), Positives = 28/56 (50%)
Frame = -2
Query: 510 NNFCAPLNKALSFGVSPTSMILAPASNCMMSPDVTIGEIPSSINVPLFDARITRIQ 343
N C +K + P ++I +P+S S ++ + PSS+NV + R+ +Q
Sbjct: 368 NGLCRNDSKCRFLMILPETIIKSPSSFIGDSYNIANIDPPSSMNVQSSEFRVGEVQ 423
>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 25.4 bits (53), Expect = 9.1
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = -1
Query: 691 DTRTQSISFXEQFIEQQHNKTGHKQLYDNEEAHSG 587
DTR Q + F +Q+IE N + LY E G
Sbjct: 169 DTRGQKLVFEDQYIELTTNMVENYNLYGLAETIHG 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,158,862
Number of Sequences: 5004
Number of extensions: 65367
Number of successful extensions: 177
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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