Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P04_F_E04
         (699 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    93   3e-20
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    87   2e-18
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    66   5e-12
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    54   1e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    48   1e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    28   1.1  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 93.5 bits (222), Expect = 3e-20
 Identities = 51/134 (38%), Positives = 78/134 (58%), Gaps = 6/134 (4%)
 Frame = +1

Query: 223 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 390
           + +Q+AEF+EAF L D D+DG I  N+L     SLG+  +  EL +M+ E     +G I+
Sbjct: 7   TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66

Query: 391 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDE 564
           FT+ LT+ A +M   +D ++ V  AFK FD++G   I  E L H L + G++ S +EV +
Sbjct: 67  FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125

Query: 565 AYDQMDIDDKGYID 606
              + D D  G I+
Sbjct: 126 MIREADTDGDGVIN 139



 Score = 28.7 bits (61), Expect = 0.85
 Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = +1

Query: 478 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 636
           D  G ID +E L        D  + +EV EA+   D D  GYI   +L  +LT+
Sbjct: 60  DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 87.0 bits (206), Expect = 2e-18
 Identities = 47/130 (36%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
 Frame = +1

Query: 178 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 357
           ++ AKR  S  F+  +  Q+ E KEAF L+D D DG IG+ D++    SL + ASE  ++
Sbjct: 28  AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87

Query: 358 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 531
            M    + PIN    LT   + +   S  +D ++ AF TFD  + GKI    +R AL + 
Sbjct: 88  HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146

Query: 532 GDKFSADEVD 561
           GD+    EV+
Sbjct: 147 GDRMDPQEVE 156


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 66.1 bits (154), Expect = 5e-12
 Identities = 40/140 (28%), Positives = 72/140 (51%), Gaps = 2/140 (1%)
 Frame = +1

Query: 223 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 402
           S++Q  E KEAF L D DKDG+I  + + +   SLG   ++ EL ++  E    I+  + 
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 403 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQ 576
           ++  +N++     E++  I AF+ FD++  G I++ +    + T G+K S +EV     +
Sbjct: 64  MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122

Query: 577 MDIDDKGYIDTTKLIAMLTA 636
            D  + G  D    +  + A
Sbjct: 123 ADPTNSGSFDYYDFVQRIMA 142


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 36/142 (25%), Positives = 68/142 (47%), Gaps = 6/142 (4%)
 Frame = +1

Query: 223 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 390
           +++Q  +  EAF+L D DKD  I  ++LRA   +LG  A + E+ +++ +      G + 
Sbjct: 32  TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91

Query: 391 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDKFSADEVDE 564
               + +   ++    D  + +  AF+ F  DE GKI    LR       +     E++ 
Sbjct: 92  MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150

Query: 565 AYDQMDIDDKGYIDTTKLIAML 630
             ++ D+D  G I+  + IA++
Sbjct: 151 MIEEFDLDQDGEINEQEFIAIM 172


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 34/136 (25%), Positives = 64/136 (47%), Gaps = 4/136 (2%)
 Frame = +1

Query: 244 FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 423
           +K+AF L D    G I K  +     + G+  +  E+ E+       ++  Q L +  NR
Sbjct: 8   YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NR 66

Query: 424 MSGGS--DEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 591
            +G     + +  +  F+ FD++  G I    LR+ L + G+K S +E+DE    + + D
Sbjct: 67  PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD 126

Query: 592 KGYIDTTKLIAMLTAS 639
            G ++    + M+ A+
Sbjct: 127 -GMVNYHDFVQMILAN 141



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 18/42 (42%), Positives = 26/42 (61%)
 Frame = +1

Query: 241 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 366
           EF + FQ+ D D  G+IG  +LR    SLG   S +E+DE++
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = +1

Query: 550 DEVDEAYDQMDIDDKGYID 606
           +E +EA+D  D+  KGYID
Sbjct: 12  EEAEEAFDLFDVTHKGYID 30



 Score = 27.1 bits (57), Expect = 2.6
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +1

Query: 241 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 366
           E +EAF L D    G I   DLR +   LG   ++++L  M+
Sbjct: 13  EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,897,283
Number of Sequences: 5004
Number of extensions: 28372
Number of successful extensions: 137
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)