Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P03_pT_E23
(719 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 84 2e-17
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 52 6e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 52 6e-08
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 51 2e-07
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 36 0.006
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 35 0.010
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.018
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 32 0.072
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 32 0.095
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 2.7
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 27 2.7
SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 3.6
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 26 6.2
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 25 8.2
SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 8.2
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 84.2 bits (199), Expect = 2e-17
Identities = 38/111 (34%), Positives = 72/111 (64%)
Frame = -1
Query: 719 MGQLFDDSELQALIDXNDPEXSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDRE 540
+GQ +ELQ +I+ D + +G I+F F + + +++ D E +E++EAF+++D++
Sbjct: 41 LGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNE---EEVREAFKVFDKD 97
Query: 539 GNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMT 387
GNGYIT L +L +L ++LS ++ +I E DTDG G ++++EF +++
Sbjct: 98 GNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVIS 148
Score = 62.5 bits (145), Expect = 6e-11
Identities = 30/73 (41%), Positives = 49/73 (67%)
Frame = -1
Query: 575 ELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 396
E +EAF L+DR+ +G IT++ L ++ +L + A+L +I E+D DG+GT+DF EF+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 395 MMTGD*AQRSKDS 357
MM A++ KD+
Sbjct: 73 MM----ARKMKDT 81
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 52.4 bits (120), Expect = 6e-08
Identities = 26/89 (29%), Positives = 52/89 (58%)
Frame = -1
Query: 662 EXSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDD 483
E I+ F + S+ L E ++E +E +AFR++D++ +GYI T+ + + L +
Sbjct: 53 ELGDAIDEKKFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGE 109
Query: 482 KLSNADLDGIIAEIDTDGSGTVDFDEFME 396
KLS+ ++ ++ E D SG+ D+ +F++
Sbjct: 110 KLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Score = 35.9 bits (79), Expect = 0.006
Identities = 20/63 (31%), Positives = 37/63 (58%)
Frame = -1
Query: 575 ELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 396
E+KEAF LYD + +G I TS + +L +L +++A+L A++ + +D +FM
Sbjct: 10 EMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAEL----AKLSNELGDAIDEKKFMS 65
Query: 395 MMT 387
++
Sbjct: 66 FVS 68
Score = 25.8 bits (54), Expect = 6.2
Identities = 9/30 (30%), Positives = 17/30 (56%)
Frame = -1
Query: 719 MGQLFDDSELQALIDXNDPEXSGKINFDGF 630
+G+ D+E+Q ++ DP SG ++ F
Sbjct: 107 LGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 52.4 bits (120), Expect = 6e-08
Identities = 26/105 (24%), Positives = 53/105 (50%)
Frame = -1
Query: 704 DDSELQALIDXNDPEXSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYI 525
+ SE+ ++ D G + + F + + + E D +E+K AF L+D + G I
Sbjct: 71 EKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKI 127
Query: 524 TTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 390
+ L+ + L++ + + +L+ +I E D D G ++ EF+ +M
Sbjct: 128 SLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIM 172
Score = 42.3 bits (95), Expect = 7e-05
Identities = 20/74 (27%), Positives = 39/74 (52%)
Frame = -1
Query: 608 LEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDG 429
L E E +Q++ EAF+L+D + + I L+ + AL +++ I+ + D G
Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTG 86
Query: 428 SGTVDFDEFMEMMT 387
G + ++F+ +MT
Sbjct: 87 KGYLQMEDFVRVMT 100
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 50.8 bits (116), Expect = 2e-07
Identities = 31/101 (30%), Positives = 54/101 (53%), Gaps = 6/101 (5%)
Frame = -1
Query: 668 DPEXSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL 489
D + G ++F F N S F + E ++LK AF++YD + +GYI+ L +L +
Sbjct: 66 DEDGGGDVDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMM 122
Query: 488 ------DDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMTG 384
+D+L +D I E+D D G + F+EF ++++G
Sbjct: 123 VGTNLREDQLQQI-VDKTIMEVDKDRDGKISFEEFKDIVSG 162
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/64 (23%), Positives = 31/64 (48%)
Frame = -1
Query: 578 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFM 399
+ +++ F D +G I + I + + L++ + + +D DG G VDF EF+
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLASR----LFSVVDEDGGGDVDFQEFI 79
Query: 398 EMMT 387
++
Sbjct: 80 NSLS 83
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.9 bits (79), Expect = 0.006
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = -1
Query: 578 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 438
QELKEAF L D++G+G I +K +L +L+ S ++ + I+
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 35.1 bits (77), Expect = 0.010
Identities = 18/60 (30%), Positives = 35/60 (58%)
Frame = -1
Query: 569 KEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 390
K+AF L+DR G G I +++ ++L A + A+ I EI++ VD ++F++++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQVL 64
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 34.3 bits (75), Expect = 0.018
Identities = 21/64 (32%), Positives = 32/64 (50%)
Frame = -1
Query: 584 MQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDE 405
M +E +EAF L+D GYI L+ A L + L+ L ++ T+G V +E
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK--VSREE 67
Query: 404 FMEM 393
F E+
Sbjct: 68 FAEL 71
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 32.3 bits (70), Expect = 0.072
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -1
Query: 602 EEDAEAMQQELKEAFRLYDREGNGYITTSTLKE 504
++D M Q LKE Y+ E N IT+ TLKE
Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 31.9 bits (69), Expect = 0.095
Identities = 22/55 (40%), Positives = 29/55 (52%)
Frame = -1
Query: 566 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEF 402
E FR + E NGY++ S +L + KLS+ L+ I D D G DFDEF
Sbjct: 11 EIFRGLNPE-NGYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGMFDFDEF 62
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 27.1 bits (57), Expect = 2.7
Identities = 18/59 (30%), Positives = 23/59 (38%)
Frame = -1
Query: 566 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 390
+ F D E GYI A L + +L I +DT G +D DEF M
Sbjct: 306 QLFSKIDNENKGYIVGGEAVPFFMA--SHLDSEELARIWDTVDTQDRGYIDKDEFAVAM 362
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 27.1 bits (57), Expect = 2.7
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = -3
Query: 225 RFCLSCRNVLVIYVPASCRSRTYLNXFQRVIFGI 124
R CL+ R ++ +Y+ C + N F++++F I
Sbjct: 533 RICLNPRALVELYINYDCDRSSTTNVFEQLLFSI 566
>SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 418
Score = 26.6 bits (56), Expect = 3.6
Identities = 14/55 (25%), Positives = 26/55 (47%)
Frame = -1
Query: 641 FDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKL 477
F F + ++ +D A+ Q + +F +R G G I T +++ + DKL
Sbjct: 356 FQNFSSPSTSLSAPKDTPAVIQSINTSFLNNERAGRGNIKTVDQNQLINRIVDKL 410
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1183
Score = 25.8 bits (54), Expect = 6.2
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = -1
Query: 554 LYDREGNGYITT-STLKEILAALDDKLSNADLDGII-AEIDTDGSGTVDFDEFMEMMTGD 381
LYD+E GY+T + E++ + D+ N D + ++ + SG V E+
Sbjct: 1055 LYDKEKRGYVTLFPSGSEVVQFVPDRTYNIDSEYVVYLSLHESRSGRVYMSPLTEISPEH 1114
Query: 380 *AQRSKDS 357
A+ +K++
Sbjct: 1115 LARLAKNT 1122
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 25.4 bits (53), Expect = 8.2
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = -1
Query: 668 DPEXSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL 489
D + +G I+F F S E + +L AF+LYD + NG I+ + I+ A+
Sbjct: 73 DADKNGYIDFKEFICALSVTSRGE----LNDKLIWAFQLYDLDNNGLISYDEMLRIVDAI 128
>SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 8.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -1
Query: 545 REGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 438
RE GY+ T + D L+NA+L I+A+ID
Sbjct: 107 REETGYVGTVMDSTTVMYNDPGLTNANLKIILADID 142
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,581,394
Number of Sequences: 5004
Number of extensions: 47337
Number of successful extensions: 151
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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