Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P03_F_P15
(662 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 153 2e-38
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 144 1e-35
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr... 106 2e-24
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 97 2e-21
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 95 1e-20
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 28 1.4
SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 27 1.8
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 27 3.2
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 5.6
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 25 7.4
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 7.4
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 25 9.7
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 153 bits (372), Expect = 2e-38
Identities = 78/163 (47%), Positives = 103/163 (63%), Gaps = 3/163 (1%)
Frame = +1
Query: 181 KPYDGKKACWVPDEKEGFVQGEIKATKGD---LVTVNLPGGETKDFKKDQVAQVNPPKYE 351
K D K+ W+ D + F + IK D +V N E K +D++ VNP K++
Sbjct: 19 KDIDDKRWVWISDPETAFTKAWIKEDLPDKKYVVRYNNSRDE-KIVGEDEIDPVNPAKFD 77
Query: 352 KCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGK 531
+ DM+ LTYLN+ +V YNL+QRY IYTYSGLF VA+NPY P+YT +LY+ K
Sbjct: 78 RVNDMAELTYLNEPAVTYNLEQRYLSDQIYTYSGLFLVAVNPYCGLPIYTKDIIQLYKDK 137
Query: 532 RRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTXNT 660
+ PH+FAI+D AY N+L N ENQS+L+TGESGAGKT NT
Sbjct: 138 TQERKLPHVFAIADLAYNNLLENKENQSILVTGESGAGKTENT 180
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 144 bits (349), Expect = 1e-35
Identities = 72/159 (45%), Positives = 95/159 (59%), Gaps = 1/159 (0%)
Frame = +1
Query: 187 YDGKKACWVPDEKEGFVQGEIKATKGDLVTVNLP-GGETKDFKKDQVAQVNPPKYEKCED 363
+D + W+PD KE FV+ I G+ V L G + +VNPPK++ +D
Sbjct: 34 FDERTWIWIPDSKESFVKAWIVEDLGEKYRVKLERDGSERIVDGFDAEKVNPPKFDMVDD 93
Query: 364 MSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGKRRSE 543
M+ LT LN+ SV+ NL QRY LIYTYSGLF VA+NPY P+Y + Y+ K+ E
Sbjct: 94 MAALTCLNEPSVVNNLTQRYEKDLIYTYSGLFLVAVNPYCHLPIYGDDVVRKYQSKQFKE 153
Query: 544 VPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTXNT 660
PHIF +D AY ++L NQS+L+TGESGAGKT T
Sbjct: 154 TKPHIFGTADAAYRSLLERRINQSILVTGESGAGKTETT 192
>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1516
Score = 106 bits (255), Expect = 2e-24
Identities = 57/163 (34%), Positives = 92/163 (56%), Gaps = 14/163 (8%)
Frame = +1
Query: 205 CWVPDEKEGFVQGEIKATKGDLVTVNLPGGETKDFKKDQVAQVNPPKYE----------- 351
CW+PDE+ ++ G IK + + L + + + + + YE
Sbjct: 12 CWIPDEQSQWIPGSIKDCRVEGEKAFLTVQDENENETVITVKPDDLNYEGRNGLPFLRSI 71
Query: 352 --KCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFP-VYTTRCAKLY 522
+D+++L+YLN+ SVL L RY IYTYSG+ +A+NP++R P +YT + Y
Sbjct: 72 NSDADDLTDLSYLNEPSVLDALSTRYNQLQIYTYSGIVLIAVNPFQRLPNLYTHEIVRAY 131
Query: 523 RGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKT 651
K R E+ PH++AI++ +Y M H+NQ+++I+GESGAGKT
Sbjct: 132 SEKSRDELDPHLYAIAEDSYKCMNQEHKNQTIIISGESGAGKT 174
>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1471
Score = 97.5 bits (232), Expect = 2e-21
Identities = 56/155 (36%), Positives = 83/155 (53%), Gaps = 6/155 (3%)
Frame = +1
Query: 205 CWVPDEKEGFVQG---EIKATKGDLV--TVNLPGGETKDFKKDQVAQVNPPKYEKCEDMS 369
CWV + + EIK G V TV G + Q+ N + E D++
Sbjct: 12 CWVSNNNGHWDAARLIEIKDNGGGKVVATVAKSSGVLETVNYQQLQNRNIGQSESPSDLT 71
Query: 370 NLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFP-VYTTRCAKLYRGKRRSEV 546
NL YLN+ SVL+ L RY +K IYTYSG+ V+INPY+ P Y K + +
Sbjct: 72 NLPYLNEPSVLHALHNRYNNKQIYTYSGIVLVSINPYQNLPEFYNDNLIKHFHKDPEAAK 131
Query: 547 PPHIFAISDGAYVNMLTNHENQSMLITGESGAGKT 651
PH+++I+ Y + T+ +NQ+++++GESGAGKT
Sbjct: 132 VPHLYSIASSCYHALTTDSKNQTIIVSGESGAGKT 166
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 94.7 bits (225), Expect = 1e-20
Identities = 41/98 (41%), Positives = 65/98 (66%)
Frame = +1
Query: 358 EDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTTRCAKLYRGKRR 537
+D++ L+ + D + NL+ R+ + IYTY G +++NP++ +YT K Y+GK R
Sbjct: 43 DDLTLLSKITDEEINKNLELRFRNGEIYTYIGHVLISVNPFRDLGIYTMDILKSYQGKNR 102
Query: 538 SEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKT 651
E PH++AI++ AY M + HENQ ++I+GESGAGKT
Sbjct: 103 LETSPHVYAIAENAYYQMKSYHENQCIIISGESGAGKT 140
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 27.9 bits (59), Expect = 1.4
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1
Query: 601 HENQSMLITGESGAGKT 651
HENQ ++ GE+G+GKT
Sbjct: 89 HENQIIVFVGETGSGKT 105
>SPCC1919.03c |||AMP-activated protein kinase beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 298
Score = 27.5 bits (58), Expect = 1.8
Identities = 17/51 (33%), Positives = 23/51 (45%)
Frame = +1
Query: 337 PPKYEKCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRF 489
PP EKC SN Y D SVL N + L + L +A++ R+
Sbjct: 233 PPHLEKCILNSNTAYKEDQSVLPNPNHVLLNHLAAANTQLGVLALSATTRY 283
>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 26.6 bits (56), Expect = 3.2
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -3
Query: 81 ANETFGLVETREHXPWSL 28
A++ FG +REH PW+L
Sbjct: 609 AHDNFGCGSSREHAPWAL 626
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 25.8 bits (54), Expect = 5.6
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -1
Query: 140 YGVGSGSSPSWTTGFGIFGG 81
+G GSG P GFG FGG
Sbjct: 193 FGGGSGGPPPGPGGFGGFGG 212
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 25.4 bits (53), Expect = 7.4
Identities = 8/16 (50%), Positives = 14/16 (87%)
Frame = +1
Query: 604 ENQSMLITGESGAGKT 651
+NQ +++ GE+G+GKT
Sbjct: 507 DNQVLIVVGETGSGKT 522
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.4 bits (53), Expect = 7.4
Identities = 25/102 (24%), Positives = 43/102 (42%), Gaps = 6/102 (5%)
Frame = +1
Query: 298 TKDFKKDQVAQVNPPKYEKCEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINP 477
TK + D + + + P K D ++ VL+N Q YY+ + + ++ NP
Sbjct: 935 TKFLECDFLVKQSEPLDRKRIDAISVILSECGFVLFNFSQPYYYYFSFLLAEMYLRYGNP 994
Query: 478 YKRFPV-YTTRCAKLYRGK-----RRSEVPPHIFAISDGAYV 585
R+ V + + R K R S+V +F I D + V
Sbjct: 995 SLRYSVMFLASYCFVTRRKPEFLLRISQVDSDLFVIKDRSAV 1036
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.0 bits (52), Expect = 9.7
Identities = 17/48 (35%), Positives = 24/48 (50%), Gaps = 2/48 (4%)
Frame = +2
Query: 470 STLTRDSPCTRHDAPSCTVASVVRKCHLTSSPFP--TALTSTC*PTTR 607
ST T P + + PS +S+ H TS+ FP +A +S PT R
Sbjct: 234 STSTPAPPNSNNANPSTLFSSIPSSRHTTSNHFPSNSAQSSLFSPTAR 281
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,812,984
Number of Sequences: 5004
Number of extensions: 61020
Number of successful extensions: 207
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -