Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P03_F_N11
(684 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 258 5e-70
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 254 1e-68
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 126 4e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 108 6e-25
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 258 bits (632), Expect = 5e-70
Identities = 115/199 (57%), Positives = 142/199 (71%)
Frame = +1
Query: 88 LGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFV 267
+GQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++V
Sbjct: 9 VGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYV 68
Query: 268 DLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVXLVLDRIRKLADQ 447
DLEP V+D+VRTG YR LFHP QLITGKEDA+NNYARGHYT+GKE+V V D+IR++AD
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128
Query: 448 CTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSXLEFAIYXXXXXXXXXXXXYNSI 627
C+GLQGFL+FH LL+ERL+++Y KKS L+F++Y YNS+
Sbjct: 129 CSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSV 188
Query: 628 XXXXXXXXXSDCAFMVDNE 684
+DC FMVDNE
Sbjct: 189 LTTHATLDLADCTFMVDNE 207
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 254 bits (621), Expect = 1e-68
Identities = 117/204 (57%), Positives = 145/204 (71%), Gaps = 5/204 (2%)
Frame = +1
Query: 88 LGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVP 252
+GQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK VP
Sbjct: 9 VGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVP 67
Query: 253 RAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVXLVLDRIR 432
R+++VDLEP V+D+VRTG Y+ LFHP Q++TGKEDA+NNYARGHYT+GKE++ VL+RIR
Sbjct: 68 RSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIR 127
Query: 433 KLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSXLEFAIYXXXXXXXXXXX 612
++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++Y
Sbjct: 128 RMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVE 187
Query: 613 XYNSIXXXXXXXXXSDCAFMVDNE 684
YNS+ SDC FMVDNE
Sbjct: 188 PYNSVLTTHATLDNSDCTFMVDNE 211
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 126 bits (303), Expect = 4e-30
Identities = 67/198 (33%), Positives = 95/198 (47%)
Frame = +1
Query: 91 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 270
GQ G Q+G A W EHG+ G T + N +F+E GK+VPRAV VD
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67
Query: 271 LEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVXLVLDRIRKLADQC 450
LEP +D V++G + LF P +I G+ A N +A+GHYT G E+ VLD +R+ A+ C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 451 TGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSXLEFAIYXXXXXXXXXXXXYNSIX 630
LQGF + H LL+ ++ +Y + F++ YN+
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187
Query: 631 XXXXXXXXSDCAFMVDNE 684
SD F +DNE
Sbjct: 188 SMHQLVENSDETFCIDNE 205
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 108 bits (260), Expect = 6e-25
Identities = 63/200 (31%), Positives = 100/200 (50%), Gaps = 3/200 (1%)
Frame = +1
Query: 91 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 270
GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +D
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68
Query: 271 LEPTVVDEVRTGTYRQLFHPXQLITGKE--DAANNYARGHYTIGKEIVXLVLDRIRKLAD 444
LEP VV+ + + TY L++P ++ K A NN+A G Y+ + I ++D I + AD
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 445 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSXLEFAIY-XXXXXXXXXXXXYN 621
L+GF + H L+ERL+ Y KK ++++ YN
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187
Query: 622 SIXXXXXXXXXSDCAFMVDN 681
S+ +D ++DN
Sbjct: 188 SLLALKRLTLNADSVVVLDN 207
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 143 STASSLMARCPQTRPSGVETILSTLSSARPELAST 247
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 162 WPDAHRQDHRGWRRFFQHFLQR 227
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,532,318
Number of Sequences: 5004
Number of extensions: 49401
Number of successful extensions: 118
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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