Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P03_F_M02
(695 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q63CQ6 Cluster: Multifunctional nonribosomal peptide sy... 36 0.72
UniRef50_Q4C639 Cluster: Amino acid adenylation; n=1; Crocosphae... 36 0.95
UniRef50_A5GED1 Cluster: AMP-dependent synthetase and ligase; n=... 35 1.7
UniRef50_A0DMS6 Cluster: Chromosome undetermined scaffold_57, wh... 35 1.7
UniRef50_A4R850 Cluster: Putative uncharacterized protein; n=2; ... 35 1.7
UniRef50_Q21E99 Cluster: Amino acid adenylation; n=2; Bacteria|R... 33 8.8
UniRef50_Q5CWI6 Cluster: Beta-adaptin AP complex subunit-related... 33 8.8
UniRef50_A6RUV8 Cluster: Putative uncharacterized protein; n=1; ... 33 8.8
>UniRef50_Q63CQ6 Cluster: Multifunctional nonribosomal peptide
synthetase; n=1; Bacillus cereus E33L|Rep:
Multifunctional nonribosomal peptide synthetase -
Bacillus cereus (strain ZK / E33L)
Length = 3044
Score = 36.3 bits (80), Expect = 0.72
Identities = 38/170 (22%), Positives = 78/170 (45%), Gaps = 3/170 (1%)
Frame = +2
Query: 185 MVTMNRGYYDVFMRTCARSPGNVAIRQYECGKYHTYAYSELYGVCEYVSQNLQQLKCNK- 361
M +N +F ++ + N+A Q+E T YSEL Y+ L + K
Sbjct: 444 MFPINETLIALFEKSVNLNANNIAA-QFE---NETITYSELNERANYIGSELLKNGVTKE 499
Query: 362 GVIALVSEKNAIIPSVIAAAHKCCTSFIFLNSPQDIEIITGKIKLSAVIVINKNRDNVQL 541
++ ++SE + + + + A K +++ ++ +E ++ IK S + + +++N ++
Sbjct: 500 SIVGIISESSIGMIAAVLGALKAGAAYLPIDPSMPLERLSYIIKDSKMDALITSQENFEI 559
Query: 542 ELFGKKPDTTASMFDLNILFYSCDTTAENNFVSQHS--FIATTSGSTGEP 685
+ ++F LN L TA +++S ++ TSGSTG P
Sbjct: 560 TDLSED-----NIFLLNSL-EGMSATAPRRTATKNSLAYVIYTSGSTGNP 603
>UniRef50_Q4C639 Cluster: Amino acid adenylation; n=1; Crocosphaera
watsonii WH 8501|Rep: Amino acid adenylation -
Crocosphaera watsonii
Length = 2281
Score = 35.9 bits (79), Expect = 0.95
Identities = 39/160 (24%), Positives = 73/160 (45%), Gaps = 4/160 (2%)
Frame = +2
Query: 218 FMRTCARSPGNVAIRQYECGKYHTYAYSELYGVCEYVSQNLQQLKCNKGV-IALVSEKNA 394
F + P +A++ K + Y Y +L ++++L L +K +AL + N
Sbjct: 1512 FEEQVKKYPDKIAVQS----KDNQYTYQKLNTEANKIAKSLLNLGIDKQAKVALFFDHNV 1567
Query: 395 IIPSVIAAAHKCCTSFIFL--NSPQDIEIITGKIKLSAVIVINK-NRDNVQLELFGKKPD 565
+ + + K ++ + N PQD I T + + VI+ N+ N DN++ GK P
Sbjct: 1568 SMIAAMLGILKAGKIYVPIDPNYPQDRVIYTLEDSCAEVILTNQINSDNIKAITHGKLP- 1626
Query: 566 TTASMFDLNILFYSCDTTAENNFVSQHSFIATTSGSTGEP 685
++ LN + + + ++ +I TSGSTG+P
Sbjct: 1627 -IINIDKLNDVAVEINLEISPDTLA---YILYTSGSTGQP 1662
>UniRef50_A5GED1 Cluster: AMP-dependent synthetase and ligase; n=2;
Geobacter|Rep: AMP-dependent synthetase and ligase -
Geobacter uraniumreducens Rf4
Length = 603
Score = 35.1 bits (77), Expect = 1.7
Identities = 26/107 (24%), Positives = 47/107 (43%), Gaps = 4/107 (3%)
Frame = +2
Query: 212 DVFMRTCARSPGNVAIRQYECGKYHTYAYSELYGVCEYVSQNLQQLKCNKGV-IALVSEK 388
D+ + AR G +A++ + GK+ T +Y+E Y ++ L++ G +A++SE
Sbjct: 11 DMLRQNAARFQGKLALKYRKQGKFVTLSYAEFYERALMAARGLKKCNVKPGERVAILSEN 70
Query: 389 NA---IIPSVIAAAHKCCTSFIFLNSPQDIEIITGKIKLSAVIVINK 520
A I I N+P+ IE + + V V +K
Sbjct: 71 RAGWVIADMGILTVGGVTVPIYPTNTPEQIEYVLNHSEARIVFVSSK 117
>UniRef50_A0DMS6 Cluster: Chromosome undetermined scaffold_57, whole
genome shotgun sequence; n=2; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_57,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 314
Score = 35.1 bits (77), Expect = 1.7
Identities = 16/67 (23%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
Frame = +1
Query: 280 ISYICLFRIIRSLRIRVAELATVKMQQRCHSIGFGKECNHTFGYSSGTQMLYLFYIFE-F 456
+ ++ L +I + + E+ K Q+C + + K C+ T + +Y +Y E F
Sbjct: 34 VIFVALGIVITVINNNIQEVTIYKYDQKCSPVEYNKRCSFTQNLDNMKAPIYFYYELENF 93
Query: 457 SSRHRDY 477
HR Y
Sbjct: 94 YQNHRRY 100
>UniRef50_A4R850 Cluster: Putative uncharacterized protein; n=2;
Magnaporthe grisea|Rep: Putative uncharacterized protein
- Magnaporthe grisea (Rice blast fungus) (Pyricularia
grisea)
Length = 612
Score = 35.1 bits (77), Expect = 1.7
Identities = 23/85 (27%), Positives = 35/85 (41%), Gaps = 1/85 (1%)
Frame = +2
Query: 377 VSEKNAIIPSVIAAAHKCCTSFIFLNSPQDIEII-TGKIKLSAVIVINKNRDNVQLELFG 553
+S+ N P + H L + +I G L I +N D+ +LE+F
Sbjct: 4 ISQSNDA-PKQVKRGHASLQPIYTLQRTLSVIVIGAGASGLLLAYKIQRNFDDFELEVFE 62
Query: 554 KKPDTTASMFDLNILFYSCDTTAEN 628
K PD T + ++ SCD A N
Sbjct: 63 KNPDVTGTWYENRYPGCSCDVPAHN 87
>UniRef50_Q21E99 Cluster: Amino acid adenylation; n=2; Bacteria|Rep:
Amino acid adenylation - Saccharophagus degradans (strain
2-40 / ATCC 43961 / DSM 17024)
Length = 3111
Score = 32.7 bits (71), Expect = 8.8
Identities = 40/170 (23%), Positives = 73/170 (42%), Gaps = 4/170 (2%)
Frame = +2
Query: 188 VTMNRGYYDVFMRTCARSPGNVAIRQYECGKYHTYAYSELYGVCEYVSQNLQQLKCNKG- 364
+T N+ D+ A V + E GK Y+EL ++ NLQ + KG
Sbjct: 2277 ITENKTIIDLIDGHSASGKDAVVLPN-EMGKARKATYAELTAKSNRLAHNLQNMGIGKGS 2335
Query: 365 VIALVSEKNAIIPSVIAAAHKCCTSFIFLNS--PQD-IEIITGKIKLSAVIVINKNRDNV 535
++AL K+ + + K +++ L+ P D I + K++ +V N + +
Sbjct: 2336 IVALCLPKSPELIMTLLGVLKAGAAYLPLDPDYPADRISYMLNHAKVNLALVDNHTQTRL 2395
Query: 536 QLELFGKKPDTTASMFDLNILFYSCDTTAENNFVSQHSFIATTSGSTGEP 685
E GK T + L+ + + A ++ +++ TSGSTG+P
Sbjct: 2396 S-EWQGKA--LTLADKSLSTITKTSRLAAASH--DDLAYVIYTSGSTGKP 2440
>UniRef50_Q5CWI6 Cluster: Beta-adaptin AP complex subunit-related;
ARM/HEAT repeat protein; n=3; Cryptosporidium|Rep:
Beta-adaptin AP complex subunit-related; ARM/HEAT repeat
protein - Cryptosporidium parvum Iowa II
Length = 884
Score = 32.7 bits (71), Expect = 8.8
Identities = 22/101 (21%), Positives = 47/101 (46%), Gaps = 2/101 (1%)
Frame = +2
Query: 194 MNRGYYDVFMRTCARSPGNVAIRQYECGKYHTYAYSELYGVCEYVSQNLQQL--KCNKGV 367
+ R +Y + + + + G + + K+ Y Y + V +YV NL + K + +
Sbjct: 514 VGRYFYMILVEKLSFNTGGICSFLWIMAKFPDYVYCDKGEVLDYVVTNLLDIFEKDSSNI 573
Query: 368 IALVSEKNAIIPSVIAAAHKCCTSFIFLNSPQDIEIITGKI 490
LV+ + I +I + CC + +SP+D+ + G++
Sbjct: 574 ENLVNRPSNIFSILITS---CCR--LLFDSPEDVRPVLGRL 609
>UniRef50_A6RUV8 Cluster: Putative uncharacterized protein; n=1;
Botryotinia fuckeliana B05.10|Rep: Putative
uncharacterized protein - Botryotinia fuckeliana B05.10
Length = 176
Score = 32.7 bits (71), Expect = 8.8
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = +2
Query: 341 QQLKCNKGVIALVSEKNAIIPSVIAAAHKCCTSFIFLNSPQDIEIITGKIKLSAVIVIN 517
Q+ N V+A S I+P++ A H C TS NS ++I + IK +++ N
Sbjct: 87 QKANSNSQVVAFTSFSKVIMPTIFDACHSCKTS--SANSGKNIRCLIRDIKHKMLLLFN 143
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 624,277,607
Number of Sequences: 1657284
Number of extensions: 12147086
Number of successful extensions: 31128
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 29496
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31116
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 54958682807
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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