Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P03_F_L23
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 242 4e-65
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 137 1e-33
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 137 2e-33
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 136 4e-33
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 117 2e-27
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 98 1e-21
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 97 2e-21
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 32 0.094
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 31 0.22
SPBC776.06c |||spindle pole body interacting protein |Schizosacc... 27 2.7
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 26 6.1
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 242 bits (592), Expect = 4e-65
Identities = 115/214 (53%), Positives = 157/214 (73%), Gaps = 1/214 (0%)
Frame = +2
Query: 68 YDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERTVRKI 247
YDRA++VFSPDG LLQVEY QEAVR+G+TA E+K+V KLQ +KI
Sbjct: 4 YDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQKI 63
Query: 248 CLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSN 427
++D+HV +AFAGL ADARILI++A++E Q+HKL + DPV++EY+TRY+AG++QKYTQS
Sbjct: 64 AMVDNHVCLAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSG 123
Query: 428 GRRPFGISCLIGGFDY-DGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTADEV 604
G RPFG+S LI GFD D +P ++QTEP+GIY WKA A GR++K RE+LEKN+ E
Sbjct: 124 GVRPFGVSTLIAGFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKNW--KEG 181
Query: 605 ATENGAVKLAIRALLEVVQSGQKNLXIAVMRRGQ 706
+ + + LA+ +LLEVVQ+ N+ +A+M G+
Sbjct: 182 LSRDETIHLAVSSLLEVVQTASGNIELAIMDPGK 215
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 137 bits (332), Expect = 1e-33
Identities = 78/222 (35%), Positives = 121/222 (54%), Gaps = 8/222 (3%)
Frame = +2
Query: 47 FLTMSARYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQE 226
F+T S YDR + FSP+G L QVEYA EA++ GSTA EK+ + L E
Sbjct: 2 FMTRS-EYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLME 60
Query: 227 ERTVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLK 406
+V K+ +D H+ A +GLTADAR +I A+++ Q+H+ T ++P +E T+ I L
Sbjct: 61 SHSVEKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLA 120
Query: 407 QKYTQSNG------RRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVR 568
++ + RPFG++ LI G D G P L+ +EPSG Y+ ++A A G ++ +
Sbjct: 121 LRFGEGEDGEERIMSRPFGVALLIAGIDEHG-PQLYHSEPSGTYFRYEAKAIGSGSEPAK 179
Query: 569 EFLEKNYTADEVATENGAVKLAIRALLEVVQS--GQKNLXIA 688
L K + D E A L ++ L +V++ KN+ +A
Sbjct: 180 SELVKEFHKDMTLEE--AEVLILKVLRQVMEEKLDSKNVQLA 219
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 137 bits (331), Expect = 2e-33
Identities = 77/204 (37%), Positives = 112/204 (54%), Gaps = 1/204 (0%)
Frame = +2
Query: 56 MSARYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKL-QEER 232
MS YD T+FSP+G L QVEYA EA+ A EKK +KL ++E
Sbjct: 1 MSRSYDSRTTIFSPEGRLYQVEYALEAINHAGVALGIVAKDGIVLAAEKKVTSKLLEQEE 60
Query: 233 TVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQK 412
+ K+ + D+++ A AGLTADA ILIN A+ Q + T + + E + R + LKQ
Sbjct: 61 SAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQG 120
Query: 413 YTQSNGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYT 592
YTQ G RPFG+S L G+D+ LFQ+ PSG Y W+AN+ G ++ +V+ + + Y
Sbjct: 121 YTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQANSIGGNSTSVQSLMRQEYK 180
Query: 593 ADEVATENGAVKLAIRALLEVVQS 664
D E A +A++ L + + S
Sbjct: 181 DDINLDEASA--MAVKFLSKTLDS 202
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 136 bits (328), Expect = 4e-33
Identities = 79/217 (36%), Positives = 125/217 (57%), Gaps = 4/217 (1%)
Frame = +2
Query: 56 MSARYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERT 235
M+ +Y ++T FSP+G L+Q+EYA AV G T+ EKK ++L +
Sbjct: 1 MTDKYAFSLTTFSPNGKLVQIEYALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGAS 60
Query: 236 VRKICLLDDHVVMAFAGLTADARILINRAQIECQS--HKLTVEDPVTLEYITRYIAGLKQ 409
+ K+C + + M ++G+ D R+L+++++ Q+ K+ E P T + + + IA + Q
Sbjct: 61 LEKVCAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPT-KILVQEIASVMQ 119
Query: 410 KYTQSNGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNY 589
+ TQS G RPFG+S L+ G D G P L+Q +PSG Y+ WKA A G+S+ + FLEK Y
Sbjct: 120 ESTQSGGVRPFGVSLLVAGMDEKG-PSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRY 178
Query: 590 TADEVATENGAVKLAIRALLEVVQS--GQKNLXIAVM 694
DE+ ++ AV AI AL E + + N+ IAV+
Sbjct: 179 N-DELELDD-AVHTAILALKETFEGELTEDNIEIAVV 213
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 117 bits (281), Expect = 2e-27
Identities = 64/203 (31%), Positives = 106/203 (52%)
Frame = +2
Query: 65 RYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERTVRK 244
+YD T +SP G L QVEYA EA+++GS K++ +L + +K
Sbjct: 5 QYDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQ--KK 62
Query: 245 ICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQS 424
+ +DDH+ +A AGL DAR+L N + E S K P+ + + +A Q TQ
Sbjct: 63 LIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQE 122
Query: 425 NGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTADEV 604
GRRP+G+ L+ G+D G PHL + +PSG+ E+ + G +++ R ++E+N
Sbjct: 123 YGRRPYGVGFLVIGYDESG-PHLLEFQPSGLVLEYLGTSMGSRSQSARTYIERNLDTFPD 181
Query: 605 ATENGAVKLAIRALLEVVQSGQK 673
++ + A+RAL + + Q+
Sbjct: 182 SSREELILSALRALRDTLSKDQE 204
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 97.9 bits (233), Expect = 1e-21
Identities = 63/224 (28%), Positives = 107/224 (47%), Gaps = 4/224 (1%)
Frame = +2
Query: 50 LTMSARYDRAITVFSPDGHLLQVEYAQEAVRK-GSTAXXXXXXXXXXXXXEKKSVAKLQE 226
++ + +DR ITVFSP+G L QVEYA +A G T+ +KK KL +
Sbjct: 1 MSQARGFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLID 60
Query: 227 ERTVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLK 406
TV+ + + + G ADAR ++RA+ E + P+ + + + +A +
Sbjct: 61 ASTVKHVFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIA 120
Query: 407 QKYTQSNGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKN 586
Q TQ RP G++ + D + P LF+ +P+G Y +KA + G +LEK
Sbjct: 121 QVSTQRAAMRPLGVAMTLVAVDDEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKR 180
Query: 587 YTADEVATE-NGAVKLAIRALLEVVQSGQKN--LXIAVMRRGQP 709
+ D + + V+ I+AL+ + + K+ L I V+ +P
Sbjct: 181 FKKDGIPSNLTDTVETGIQALMSSLSTDFKSTELQIGVVEDDKP 224
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 97.1 bits (231), Expect = 2e-21
Identities = 61/194 (31%), Positives = 94/194 (48%), Gaps = 1/194 (0%)
Frame = +2
Query: 68 YDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERTVRKI 247
YD + FSPDG L Q EYA +AV ST EK +KL + R +I
Sbjct: 8 YDLGL-FFSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRI 66
Query: 248 CLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSN 427
+D H+ +A G D + ++ RA+ E S + P+ I + Q +T +
Sbjct: 67 GSVDRHIGIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYS 126
Query: 428 GRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEK-NYTADEV 604
RPFG+ + +D +G PHL+ EP+G+Y+ + A G+ + R LEK N+++ ++
Sbjct: 127 SVRPFGVMSFVATYDSEG-PHLYMVEPNGVYWGYNGAAAGKGRQVARNELEKLNFSSLKM 185
Query: 605 ATENGAVKLAIRAL 646
AVK A R L
Sbjct: 186 ---KDAVKEAARIL 196
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 31.9 bits (69), Expect = 0.094
Identities = 25/109 (22%), Positives = 45/109 (41%), Gaps = 2/109 (1%)
Frame = +2
Query: 260 DHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSNGRR- 436
D +V+ +G ADA L+ R Q + E ++ + + L G+R
Sbjct: 52 DDLVIGASGFEADALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLY------GKRF 105
Query: 437 -PFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLE 580
P+ + + G D +G ++ +P G Y A G +A + FL+
Sbjct: 106 FPYYVYTTVAGIDKEGKGEIYSFDPVGSYEREWCRAGGSAANFITPFLD 154
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 30.7 bits (66), Expect = 0.22
Identities = 14/72 (19%), Positives = 37/72 (51%)
Frame = +2
Query: 200 KKSVAKLQEERTVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEY 379
+ S L +TV+K+ ++ +++ AG AD + +EC+ H+L ++ +++
Sbjct: 80 RASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSA 139
Query: 380 ITRYIAGLKQKY 415
++ ++ + Y
Sbjct: 140 ASKILSNITYSY 151
>SPBC776.06c |||spindle pole body interacting protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 598
Score = 27.1 bits (57), Expect = 2.7
Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 3/41 (7%)
Frame = -1
Query: 315 FINIRASA-VNPANAITT*S--SKRHIFLTVRSSCNLATDF 202
F+N SA +NP N + T SK+ +FLT SS ++ DF
Sbjct: 261 FMNTDISADINPINVLITALLISKKVLFLTKTSSASVLADF 301
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.8 bits (54), Expect = 6.1
Identities = 27/87 (31%), Positives = 35/87 (40%), Gaps = 10/87 (11%)
Frame = -1
Query: 480 PS*SNPPIKHEIPNGRRPLL*VYFCLRP--------AMYLVIYS-NVTGSSTVNLCD*HS 328
P+ P +IPN R +L + C P MYL S N T S + L HS
Sbjct: 375 PNFPEPKFFEDIPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEVSVITLAPQHS 434
Query: 327 ICARFINIR-ASAVNPANAITT*SSKR 250
IN+ A A+ IT + KR
Sbjct: 435 FLNIVINLNPALALKSYTIITLLNGKR 461
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,872,070
Number of Sequences: 5004
Number of extensions: 59084
Number of successful extensions: 157
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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