Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P03_F_J20
(862 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 64 2e-11
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo... 62 1e-10
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 56 5e-09
SPAC31G5.06 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 32 0.091
SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr 3... 32 0.12
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 28 2.0
SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces po... 27 4.5
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 26 6.0
SPBC56F2.12 |ilv5||acetohydroxyacid reductoisomerase|Schizosacch... 26 7.9
>SPCC191.09c |gst1||glutathione S-transferase
Gst1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 229
Score = 64.1 bits (149), Expect = 2e-11
Identities = 46/139 (33%), Positives = 71/139 (51%), Gaps = 8/139 (5%)
Frame = +1
Query: 196 EQLKPEYLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKYAKGSSL-YPEDPKARVL 363
EQ PE+L LNP VPTL+D + +IWES AI+ YL +KY + P D
Sbjct: 40 EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLADKYDTERKISLPRDHPEYYK 99
Query: 364 VDQRLYFDI---GTLYQRFSDY-FYPQVFAGAPADKAKNEKVQEALQLLDKFLEGQKYVA 531
V Q L+F G ++ + + Y Q + + +NE ++ L +L+ L+ + Y+
Sbjct: 100 VIQYLFFQASGQGIIWGQAGWFSVYHQELVISAITRYRNE-IKRVLGVLEDILKDRDYLV 158
Query: 532 GPNLTVADLSLIASVSSLE 588
T+ADLS I+ + LE
Sbjct: 159 ANRFTIADLSFISWNNFLE 177
>SPCC965.07c |gst2||glutathione S-transferase
Gst2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 230
Score = 62.1 bits (144), Expect = 1e-10
Identities = 47/135 (34%), Positives = 75/135 (55%), Gaps = 10/135 (7%)
Frame = +1
Query: 193 GEQLKPEYLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKY--AKGSSLYPEDPKAR 357
GEQ E+L LNP VPTLVD + +IWES AI+ YL +KY + SL +DP+
Sbjct: 39 GEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLADKYDTDRKISLSFDDPEYY 98
Query: 358 VLVDQRLYFDI---GTLYQR--FSDYFYPQVFAGAPADKAKNEKVQEALQLLDKFLEGQK 522
L+ Q L+F G ++ + + ++F+ + A + +NE ++ L +L+ L+ +
Sbjct: 99 KLI-QYLFFQASGQGVIWGQAGWFNFFHHEPVVSA-VTRYRNE-IKRVLGVLEDILKDRD 155
Query: 523 YVAGPNLTVADLSLI 567
Y+ T+ADLS I
Sbjct: 156 YLVANKYTIADLSFI 170
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 56.4 bits (130), Expect = 5e-09
Identities = 48/176 (27%), Positives = 71/176 (40%), Gaps = 17/176 (9%)
Frame = +1
Query: 208 PEYLKLNPQHTVPTLVDDGLSIWESRAIITYLVNKYAKGSSLYPEDPKARVLVDQRLYFD 387
P Y KL+P P +VDDG++ ES AI+ +LV KY G S P + L L+
Sbjct: 40 PAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKY--GPSFKPSEEDVAELEKYELWMH 97
Query: 388 IG--------------TLYQRFSDYFYPQV---FAGAPADKAKNEKVQEALQLLDKFLEG 516
L + F+ + F K +++ L +D L
Sbjct: 98 FSEASLMPFIWASHVLDLSVNMTPIFFRYIVRQFVNGIKSKYLSKETFLNLDYIDNHLAS 157
Query: 517 QKYVAGPNLTVADLSLIASVSSLEASDIDFKKYANVKRWYETVKSTAPGYQEANEK 684
+Y AG T AD + + + + + K Y N+KRW V S P + A EK
Sbjct: 158 NEYFAGEQFTAADPQMCFPIFAAQRDYLSQKPYKNIKRWMRVV-SDRPACRIAAEK 212
>SPAC31G5.06 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 234
Score = 32.3 bits (70), Expect = 0.091
Identities = 16/48 (33%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
Frame = +2
Query: 683 KDWRHSRDLSIACLKSE-HLSTRNENLFKITSLELNLTNXAELKRXCL 823
K+ H++ +S+ CL S+ +L ++ +FK ++ELN N ++KR L
Sbjct: 58 KNHLHNKHVSLYCLLSDRYLQPPSKRIFKRWNMELNNLNCPDIKRYIL 105
>SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 334
Score = 31.9 bits (69), Expect = 0.12
Identities = 23/83 (27%), Positives = 35/83 (42%), Gaps = 4/83 (4%)
Frame = +1
Query: 460 AKNEKVQEALQLLDKFLEGQ----KYVAGPNLTVADLSLIASVSSLEASDIDFKKYANVK 627
A+ K+ +AL+ ++ E Q KY P L LS A+ S+ S + +
Sbjct: 116 AEKRKILDALKSRKEYFETQALLEKYGEQPTLAQKKLSNAAAAKSVPGSSSSSSDPMHPQ 175
Query: 628 RWYETVKSTAPGYQEANEKGLEA 696
WY+ V G E +E EA
Sbjct: 176 HWYDRVLEGLVGANENSENNREA 198
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = -2
Query: 732 SLFKHAIDKSLECLQSFLVGLLVTRSCTLNRLVPS 628
S +K++++ L CL +L+ +TRS T+ + V S
Sbjct: 51 SWYKYSLESGLVCLYLYLIYRWITRSFTVKQTVGS 85
>SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1076
Score = 26.6 bits (56), Expect = 4.5
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +1
Query: 619 NVKRWYETVKSTAPGYQEANEKGLEAFKGLVNS 717
+V + YE+++S Y+ ANEK EA +V+S
Sbjct: 739 SVHKNYESLRSQLLEYKAANEKHTEAILSVVSS 771
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 26.2 bits (55), Expect = 6.0
Identities = 33/118 (27%), Positives = 51/118 (43%)
Frame = +1
Query: 259 DGLSIWESRAIITYLVNKYAKGSSLYPEDPKARVLVDQRLYFDIGTLYQRFSDYFYPQVF 438
+ LSI ESR I + ++ S L+P D K + RL F +G + R + +
Sbjct: 263 NSLSI-ESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKF-LGPNHPRVNRETLDAIK 320
Query: 439 AGAPADKAKNEKVQEALQLLDKFLEGQKYVAGPNLTVADLSLIASVSSLEASDIDFKK 612
A +P +E A L+ V+GPN + + S ++LE DI KK
Sbjct: 321 A-SPI--LADEGAFYAKHLILDLSTLSPAVSGPN----SVKVYNSAATLEKKDILIKK 371
>SPBC56F2.12 |ilv5||acetohydroxyacid
reductoisomerase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 404
Score = 25.8 bits (54), Expect = 7.9
Identities = 31/96 (32%), Positives = 45/96 (46%), Gaps = 8/96 (8%)
Frame = +1
Query: 445 APADKAKNEKVQEALQLLDKFLE--GQKYVAGPNLTVADLSLIA-SVSSLEASDIDFKK- 612
+PA+ A NE V+EA Q L + G Y+ T A I + LEA+ +
Sbjct: 291 SPAE-AFNETVEEATQSLYPLIGKYGLDYMFAACSTTARRGAIDWTPRFLEANKKVLNEL 349
Query: 613 YANVKRWYETVKS----TAPGYQEANEKGLEAFKGL 708
Y NV+ E +S +AP Y+E +K LE + L
Sbjct: 350 YDNVENGNEAKRSLEYNSAPNYRELYDKELEEIRNL 385
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,022,212
Number of Sequences: 5004
Number of extensions: 58549
Number of successful extensions: 205
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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