Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_pT_M23
(690 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 44 2e-05
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 38 0.001
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 32 0.090
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po... 28 1.5
SPAC1687.15 |gsk3|skp1|serine/threonine protein kinase Gsk3|Schi... 28 1.5
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 27 1.9
SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac... 27 3.4
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos... 26 5.9
SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|... 25 7.8
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 44.4 bits (100), Expect = 2e-05
Identities = 17/42 (40%), Positives = 30/42 (71%)
Frame = -2
Query: 686 DVSGFPTIFWKPKDSSKKPQRYNGGRALEDFIKYVSEQATSE 561
+V+ FPTI + PKD KP+ Y G R+LE I+Y+++++ ++
Sbjct: 211 EVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSGTQ 252
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/37 (37%), Positives = 25/37 (67%)
Frame = -2
Query: 683 VSGFPTIFWKPKDSSKKPQRYNGGRALEDFIKYVSEQ 573
++GFPT+ W P D S +P +Y+ R ++ ++VSE+
Sbjct: 93 ITGFPTLIWFPPDGS-EPVQYSNARDVDSLTQFVSEK 128
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 37.9 bits (84), Expect = 0.001
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = -2
Query: 683 VSGFPTIFWKPKDSSKKPQRYNGGRALEDFIKYVSEQATSE 561
+SGFPTI + + P RY G R LED ++ + A+ E
Sbjct: 424 ISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDKHASFE 464
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 31.9 bits (69), Expect = 0.090
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 3/46 (6%)
Frame = -2
Query: 683 VSGFPTI---FWKPKDSSKKPQRYNGGRALEDFIKYVSEQATSELK 555
V GFPTI + K SS YNG R+ + K+VS+ S++K
Sbjct: 102 VQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSIPSKVK 147
>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1313
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/40 (30%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = +1
Query: 334 ERQCLCTQRM-LFISAFPWTDITQINSEVIFHCFSHYIHP 450
E + +C + +F FP + +N +V+ HC SH P
Sbjct: 594 EHRAMCAFILSVFCRGFPQGQLACLNPQVLSHCLSHLNSP 633
>SPAC1687.15 |gsk3|skp1|serine/threonine protein kinase
Gsk3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 387
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
Frame = +3
Query: 45 FNNKRTSQLM*LIYHPQVE---VYQYTTYDNTSDFYKNV 152
F N R Q+M ++ HP + Y YTT DN+ + Y N+
Sbjct: 69 FKN-RELQIMRIMKHPNIVDLIAYYYTTGDNSDEVYLNL 106
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 27.5 bits (58), Expect = 1.9
Identities = 18/42 (42%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +1
Query: 370 ISAFPWTDITQINSEV-IFHCFSHYIHPSHKYT-RRSFMESF 489
IS +TD +INS + F CF H + PS K T +F+E F
Sbjct: 780 ISRCSFTDF-EINSILNFFFCFLHTVEPSGKLTFELAFLEIF 820
>SPCC4G3.08 |psk1||serine/threonine protein kinase
Psk1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 436
Score = 26.6 bits (56), Expect = 3.4
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = -3
Query: 331 LAIVMLHSLTIIFTDNRSRITLLDLQNNTDA*TQFHLSLLSVYGA 197
LA++ LH L I++ D + LLD + + T F LS ++ GA
Sbjct: 201 LALIHLHKLGIVYRDLKPENCLLDAEGHI-LLTDFGLSKVAENGA 244
>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 449
Score = 25.8 bits (54), Expect = 5.9
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -2
Query: 659 WKPKDSSKKPQRYNGGRALEDFIKYVSEQATSELKGWDRKG 537
W+ D + P++YN LE + KY AT+ L+G G
Sbjct: 112 WQ-SDHKRLPEKYNQMVCLEAYRKYPPVCATAGLRGIQNLG 151
>SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|chr
3|||Manual
Length = 699
Score = 25.4 bits (53), Expect = 7.8
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -2
Query: 686 DVSGFPTIFWKPKDSSKKPQRYNGGRALEDFIKYVSEQATSELK 555
+VS FP I + + SSK P++ + LE IK+VS + ++
Sbjct: 465 NVSDFPQIVEQEELSSKNPRKVHFSDTLE--IKHVSRSGKANIE 506
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,981,761
Number of Sequences: 5004
Number of extensions: 63774
Number of successful extensions: 197
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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