Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_pT_D14
(870 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g12780.1 68416.m01596 phosphoglycerate kinase, putative simil... 66 3e-11
At1g56190.1 68414.m06458 phosphoglycerate kinase, putative simil... 65 7e-11
At1g79550.2 68414.m09274 phosphoglycerate kinase, putative simil... 64 2e-10
At1g79550.1 68414.m09273 phosphoglycerate kinase, putative simil... 64 2e-10
At4g28300.2 68417.m04053 hydroxyproline-rich glycoprotein family... 30 1.7
At4g28300.1 68417.m04052 hydroxyproline-rich glycoprotein family... 30 1.7
At2g07360.1 68415.m00843 SH3 domain-containing protein contains ... 29 3.1
At4g08380.1 68417.m01384 proline-rich extensin-like family prote... 29 4.0
At4g02110.1 68417.m00282 BRCT domain-containing protein contains... 29 5.3
At4g39070.1 68417.m05533 zinc finger (B-box type) family protein... 28 7.1
At4g29530.1 68417.m04212 2,3-diketo-5-methylthio-1-phosphopentan... 28 7.1
At2g24070.1 68415.m02875 expressed protein contains Pfam domain,... 28 7.1
At5g58160.1 68418.m07280 formin homology 2 domain-containing pro... 28 9.3
At4g22470.1 68417.m03245 protease inhibitor/seed storage/lipid t... 28 9.3
At1g17710.1 68414.m02192 expressed protein 28 9.3
>At3g12780.1 68416.m01596 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 481
Score = 66.1 bits (154), Expect = 3e-11
Identities = 34/80 (42%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Frame = -1
Query: 510 DGWDSXLDRNHESSSQI--LLPETKVIVWXGPAGVFEXEKFAGGTRAIXDGVVKATSNGT 337
DGW LD +S L T+ ++W GP GVFE EKFA GT AI + + + + G
Sbjct: 368 DGW-MGLDIGPDSIKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGV 426
Query: 336 VTIIGGGDTATCCAKWGTRG 277
TIIGGGD+ K G G
Sbjct: 427 TTIIGGGDSVAAVEKVGVAG 446
Score = 53.6 bits (123), Expect = 2e-07
Identities = 26/72 (36%), Positives = 45/72 (62%)
Frame = -2
Query: 764 NLLDKVNEMIIGGGMAYTFLKETKGMPIGXSLYDAEGAKIVTKLLEXAEKNNVKVHLPVD 585
+LL+K + +++GGGM +TF K +G+ +G SL + + ++ T+LL A+ V + LP D
Sbjct: 287 SLLEKCDILLLGGGMIFTFYK-AQGLSVGSSLVEEDKLELATELLAKAKAKGVSLLLPTD 345
Query: 584 FVTADXXDENAS 549
V AD +A+
Sbjct: 346 VVVADKFAPDAN 357
Score = 52.4 bits (120), Expect = 4e-07
Identities = 24/29 (82%), Positives = 27/29 (93%)
Frame = -2
Query: 272 VSHVSTGGGASLELLEGKVLPGVPALSDA 186
+SH+STGGGASLELLEGKVLPGV AL +A
Sbjct: 448 MSHISTGGGASLELLEGKVLPGVIALDEA 476
Score = 43.6 bits (98), Expect = 2e-04
Identities = 43/151 (28%), Positives = 66/151 (43%), Gaps = 3/151 (1%)
Frame = -3
Query: 868 KKELQYFAXALHEPERPFLAILGGAKVADXILLIETCLIK*MK**LEGVWHTHS*RKPKE 689
+KEL Y A+ P+RPF AI+GG+KV+ I +IE+ L K L G + K +
Sbjct: 252 QKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEK-CDILLLGGGMIFTFYKAQG 310
Query: 688 CRLVIPCMTLRELKL*PNYWXKLRKTMLKFTCP---XXXXXXXXXMKMHLVGEANVETGI 518
+ + +L+L K + + P +V + +E
Sbjct: 311 LSVGSSLVEEDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIE--- 367
Query: 517 PDGWMGLXVGPKSRELFADPIARD*SYCVXW 425
DGWMGL +GP S + F + A D + V W
Sbjct: 368 -DGWMGLDIGPDSIKTFNE--ALDTTQTVIW 395
>At1g56190.1 68414.m06458 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 478
Score = 64.9 bits (151), Expect = 7e-11
Identities = 33/80 (41%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Frame = -1
Query: 510 DGWDSXLDRNHESSSQI--LLPETKVIVWXGPAGVFEXEKFAGGTRAIXDGVVKATSNGT 337
DGW LD +S L T+ ++W GP GVFE EKFA GT A+ + + + + G
Sbjct: 365 DGW-MGLDIGPDSVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGV 423
Query: 336 VTIIGGGDTATCCAKWGTRG 277
TIIGGGD+ K G G
Sbjct: 424 TTIIGGGDSVAAVEKVGVAG 443
Score = 52.8 bits (121), Expect = 3e-07
Identities = 24/29 (82%), Positives = 27/29 (93%)
Frame = -2
Query: 272 VSHVSTGGGASLELLEGKVLPGVPALSDA 186
+SH+STGGGASLELLEGKVLPGV AL +A
Sbjct: 445 MSHISTGGGASLELLEGKVLPGVVALDEA 473
Score = 52.4 bits (120), Expect = 4e-07
Identities = 26/72 (36%), Positives = 44/72 (61%)
Frame = -2
Query: 764 NLLDKVNEMIIGGGMAYTFLKETKGMPIGXSLYDAEGAKIVTKLLEXAEKNNVKVHLPVD 585
+LL+K + +++GGGM +TF K +G+ +G SL + + ++ T LL A+ V + LP D
Sbjct: 284 SLLEKCDILLLGGGMIFTFYK-AQGLSVGSSLVEEDKLELATTLLAKAKARGVSLLLPTD 342
Query: 584 FVTADXXDENAS 549
V AD +A+
Sbjct: 343 VVIADKFAPDAN 354
Score = 47.2 bits (107), Expect = 1e-05
Identities = 42/148 (28%), Positives = 66/148 (44%)
Frame = -3
Query: 868 KKELQYFAXALHEPERPFLAILGGAKVADXILLIETCLIK*MK**LEGVWHTHS*RKPKE 689
+KEL Y A+ P+RPF AI+GG+KV+ I +IE+ L++ L G + K +
Sbjct: 249 QKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIES-LLEKCDILLLGGGMIFTFYKAQG 307
Query: 688 CRLVIPCMTLRELKL*PNYWXKLRKTMLKFTCPXXXXXXXXXMKMHLVGEANVETGIPDG 509
+ + +L+L K + + P + + IPDG
Sbjct: 308 LSVGSSLVEEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAP-DANSKIVPASAIPDG 366
Query: 508 WMGLXVGPKSRELFADPIARD*SYCVXW 425
WMGL +GP S + F + A D + V W
Sbjct: 367 WMGLDIGPDSVKTFNE--ALDTTQTVIW 392
>At1g79550.2 68414.m09274 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 401
Score = 63.7 bits (148), Expect = 2e-10
Identities = 33/77 (42%), Positives = 41/77 (53%), Gaps = 2/77 (2%)
Frame = -1
Query: 510 DGWDSXLDRNHESSSQI--LLPETKVIVWXGPAGVFEXEKFAGGTRAIXDGVVKATSNGT 337
DGW LD +S L TK I+W GP GVFE +KFA GT A+ + + + G
Sbjct: 293 DGW-MGLDIGPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGV 351
Query: 336 VTIIGGGDTATCCAKWG 286
TIIGGGD+ K G
Sbjct: 352 TTIIGGGDSVAAVEKVG 368
Score = 54.4 bits (125), Expect = 9e-08
Identities = 25/31 (80%), Positives = 28/31 (90%)
Frame = -2
Query: 278 DKVSHVSTGGGASLELLEGKVLPGVPALSDA 186
DK+SH+STGGGASLELLEGK LPGV AL +A
Sbjct: 371 DKMSHISTGGGASLELLEGKPLPGVLALDEA 401
Score = 50.4 bits (115), Expect = 2e-06
Identities = 25/72 (34%), Positives = 43/72 (59%)
Frame = -2
Query: 764 NLLDKVNEMIIGGGMAYTFLKETKGMPIGXSLYDAEGAKIVTKLLEXAEKNNVKVHLPVD 585
+LL+ V+ +++GGGM +TF K +G+ +G SL + + + L+E A+ V + LP D
Sbjct: 212 SLLNTVDILLLGGGMIFTFYK-AQGLSVGSSLVEEDKLDLAKSLMEKAKAKGVSLLLPTD 270
Query: 584 FVTADXXDENAS 549
V AD +A+
Sbjct: 271 VVIADKFAPDAN 282
Score = 47.6 bits (108), Expect = 1e-05
Identities = 41/148 (27%), Positives = 67/148 (45%)
Frame = -3
Query: 868 KKELQYFAXALHEPERPFLAILGGAKVADXILLIETCLIK*MK**LEGVWHTHS*RKPKE 689
+KEL Y A+ P++PF AI+GG+KV+ I +IE+ L+ + L G + K +
Sbjct: 177 QKELDYLVGAVANPKKPFAAIVGGSKVSTKIGVIES-LLNTVDILLLGGGMIFTFYKAQG 235
Query: 688 CRLVIPCMTLRELKL*PNYWXKLRKTMLKFTCPXXXXXXXXXMKMHLVGEANVETGIPDG 509
+ + +L L + K + + P + T IPDG
Sbjct: 236 LSVGSSLVEEDKLDLAKSLMEKAKAKGVSLLLP-TDVVIADKFAPDANSKIVPATAIPDG 294
Query: 508 WMGLXVGPKSRELFADPIARD*SYCVXW 425
WMGL +GP S + F++ A D + + W
Sbjct: 295 WMGLDIGPDSIKTFSE--ALDTTKTIIW 320
>At1g79550.1 68414.m09273 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 401
Score = 63.7 bits (148), Expect = 2e-10
Identities = 33/77 (42%), Positives = 41/77 (53%), Gaps = 2/77 (2%)
Frame = -1
Query: 510 DGWDSXLDRNHESSSQI--LLPETKVIVWXGPAGVFEXEKFAGGTRAIXDGVVKATSNGT 337
DGW LD +S L TK I+W GP GVFE +KFA GT A+ + + + G
Sbjct: 293 DGW-MGLDIGPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGV 351
Query: 336 VTIIGGGDTATCCAKWG 286
TIIGGGD+ K G
Sbjct: 352 TTIIGGGDSVAAVEKVG 368
Score = 54.4 bits (125), Expect = 9e-08
Identities = 25/31 (80%), Positives = 28/31 (90%)
Frame = -2
Query: 278 DKVSHVSTGGGASLELLEGKVLPGVPALSDA 186
DK+SH+STGGGASLELLEGK LPGV AL +A
Sbjct: 371 DKMSHISTGGGASLELLEGKPLPGVLALDEA 401
Score = 50.4 bits (115), Expect = 2e-06
Identities = 25/72 (34%), Positives = 43/72 (59%)
Frame = -2
Query: 764 NLLDKVNEMIIGGGMAYTFLKETKGMPIGXSLYDAEGAKIVTKLLEXAEKNNVKVHLPVD 585
+LL+ V+ +++GGGM +TF K +G+ +G SL + + + L+E A+ V + LP D
Sbjct: 212 SLLNTVDILLLGGGMIFTFYK-AQGLSVGSSLVEEDKLDLAKSLMEKAKAKGVSLLLPTD 270
Query: 584 FVTADXXDENAS 549
V AD +A+
Sbjct: 271 VVIADKFAPDAN 282
Score = 47.6 bits (108), Expect = 1e-05
Identities = 41/148 (27%), Positives = 67/148 (45%)
Frame = -3
Query: 868 KKELQYFAXALHEPERPFLAILGGAKVADXILLIETCLIK*MK**LEGVWHTHS*RKPKE 689
+KEL Y A+ P++PF AI+GG+KV+ I +IE+ L+ + L G + K +
Sbjct: 177 QKELDYLVGAVANPKKPFAAIVGGSKVSTKIGVIES-LLNTVDILLLGGGMIFTFYKAQG 235
Query: 688 CRLVIPCMTLRELKL*PNYWXKLRKTMLKFTCPXXXXXXXXXMKMHLVGEANVETGIPDG 509
+ + +L L + K + + P + T IPDG
Sbjct: 236 LSVGSSLVEEDKLDLAKSLMEKAKAKGVSLLLP-TDVVIADKFAPDANSKIVPATAIPDG 294
Query: 508 WMGLXVGPKSRELFADPIARD*SYCVXW 425
WMGL +GP S + F++ A D + + W
Sbjct: 295 WMGLDIGPDSIKTFSE--ALDTTKTIIW 320
>At4g28300.2 68417.m04053 hydroxyproline-rich glycoprotein family
protein
Length = 438
Score = 30.3 bits (65), Expect = 1.7
Identities = 14/49 (28%), Positives = 18/49 (36%)
Frame = +2
Query: 287 PHLAQHVAVSPPPMIVTVPLDVAFTTPSXMARVPPANFSNSNTPAGPXH 433
P Q+ PP + P V +TP + PPA P P H
Sbjct: 175 PQQHQYYMPPPPTQLQNTPAPVPVSTPPSQLQAPPAQSQFMPPPPAPSH 223
>At4g28300.1 68417.m04052 hydroxyproline-rich glycoprotein family
protein
Length = 496
Score = 30.3 bits (65), Expect = 1.7
Identities = 14/49 (28%), Positives = 18/49 (36%)
Frame = +2
Query: 287 PHLAQHVAVSPPPMIVTVPLDVAFTTPSXMARVPPANFSNSNTPAGPXH 433
P Q+ PP + P V +TP + PPA P P H
Sbjct: 233 PQQHQYYMPPPPTQLQNTPAPVPVSTPPSQLQAPPAQSQFMPPPPAPSH 281
>At2g07360.1 68415.m00843 SH3 domain-containing protein contains
Pfam profile PF00018: SH3 domain
Length = 1196
Score = 29.5 bits (63), Expect = 3.1
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +1
Query: 79 HNTVICTLLSIFVVLNKVCFV*SLNKYYYYHVLXIYASERAGTPGSTFPSSN 234
+N+V +L ++LN+ C ++ +Y YY++ I S+ TPG P+ N
Sbjct: 137 YNSVDPSLAPSLLMLNQQCEDRNVLRYVYYYLARIL-SDTGMTPGGGIPTPN 187
>At4g08380.1 68417.m01384 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 437
Score = 29.1 bits (62), Expect = 4.0
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +2
Query: 272 PCPLVPHLAQHVAVSPPPMIVTVPLDVAFTTPSXMARVPPANFSNSNTP 418
P P V +V SPPP + + P A++ P PP+ + + P
Sbjct: 124 PSPYVYKSPPYVYSSPPPYVYSSPPPYAYSPPPYAYSPPPSPYVYKSPP 172
>At4g02110.1 68417.m00282 BRCT domain-containing protein contains
Pfam domain, PF00533: BRCA1 C Terminus (BRCT) domain
Length = 1293
Score = 28.7 bits (61), Expect = 5.3
Identities = 23/72 (31%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Frame = -2
Query: 398 NSQVE-PEPSWMVS*RLHPMEPSQSLVVVILQRVAPSGEPEDKVSHVSTGGGASLELLEG 222
NS+ P PS ++ L P P+ +L V+ ++ E E+ H S + +LL
Sbjct: 385 NSEASIPPPSSLLLQELRPSSPNDNLRPVM--SISDPTESEE-AGHKSPTSELNTKLLSS 441
Query: 221 KVLPGVPALSDA 186
V+P V ALS A
Sbjct: 442 NVVPMVDALSTA 453
>At4g39070.1 68417.m05533 zinc finger (B-box type) family protein
salt-tolerance protein - Arabidopsis thaliana,
PID:e224078
Length = 242
Score = 28.3 bits (60), Expect = 7.1
Identities = 22/69 (31%), Positives = 29/69 (42%)
Frame = +1
Query: 187 ASERAGTPGSTFPSSNSRXAPPPVETCETLSSGSPLGATRCSITTTNDCDGSIGCSLYDT 366
A RA S ++ P V + E SS S T S TTT++C I + +
Sbjct: 111 AYPRASNSNSAAAFGRAKTRPKSVSS-EVPSSASNEVFTSSSSTTTSNCYYGIEENYHHV 169
Query: 367 IXDGSGSTC 393
GSGS C
Sbjct: 170 SDSGSGSGC 178
>At4g29530.1 68417.m04212 2,3-diketo-5-methylthio-1-phosphopentane
phosphatase family contains TIGRfam TIGR01489:
2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Length = 245
Score = 28.3 bits (60), Expect = 7.1
Identities = 30/129 (23%), Positives = 46/129 (35%), Gaps = 5/129 (3%)
Frame = +1
Query: 148 LNKYYYYHVLXIYASERAGTPGSTFPSSNSRXAPP-----PVETCETLSSGSPLGATRCS 312
+ K +H L SE P S + N R P P +C S G
Sbjct: 101 IEKILEHHDLVDCFSEIYTNPTSLDDNGNLRILPYHSDALPPHSCNLCPSNLCKGLVMDH 160
Query: 313 ITTTNDCDGSIGCSLYDTIXDGSGSTCEFLXLKHASRSXPHNNFSLWQ*DLRRALVISVQ 492
+ ++ D +Y + DG G C L L+ P N+ LW+ L+I +
Sbjct: 161 LRASSSNDQIPRRFIY--LGDGGGDFCPTLKLRECDFVMPRTNYPLWKKISDNPLLIKAE 218
Query: 493 XRVPSIHQE 519
+ S +E
Sbjct: 219 VKEWSSAEE 227
>At2g24070.1 68415.m02875 expressed protein contains Pfam domain,
PF04484: Family of unknown function (DUF566)
Length = 609
Score = 28.3 bits (60), Expect = 7.1
Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = +3
Query: 462 SAKSSRDFGPTSSP-IHPSGIPVSTLASPTRCIFIXXISCN 581
S +S + F +S P +HP P S ASP+R F S N
Sbjct: 283 STESLKHFSTSSLPRLHPMSAPGSRTASPSRSSFSSSSSSN 323
>At5g58160.1 68418.m07280 formin homology 2 domain-containing
protein / FH2 domain-containing protein low similarity
to SP|Q05858 Formin (Limb deformity protein) {Gallus
gallus}; contains Pfam profile PF02181: Formin Homology
2(FH2) Domain
Length = 1307
Score = 27.9 bits (59), Expect = 9.3
Identities = 16/50 (32%), Positives = 19/50 (38%)
Frame = +2
Query: 278 PLVPHLAQHVAVSPPPMIVTVPLDVAFTTPSXMARVPPANFSNSNTPAGP 427
P P L H A SPPP P + T PP +P+GP
Sbjct: 758 PAPPRLPTHSA-SPPPPTAPPPPPLGQTRAPSAPPPPPPKLGTKLSPSGP 806
>At4g22470.1 68417.m03245 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to
hydroxyproline-rich glycoprotein DZ-HRGP from Volvox
carteri f. nagariensis GP|6523547; contains Pfam profile
PF00234 Protease inhibitor/seed storage/LTP family
Length = 375
Score = 27.9 bits (59), Expect = 9.3
Identities = 15/44 (34%), Positives = 20/44 (45%)
Frame = +2
Query: 311 VSPPPMIVTVPLDVAFTTPSXMARVPPANFSNSNTPAGPXHTIT 442
+SPPP +T P +A T P+ + P S T P IT
Sbjct: 122 LSPPPPAITPPPPLATTPPALPPKPLPPPLSPPQTTPPPPPAIT 165
>At1g17710.1 68414.m02192 expressed protein
Length = 275
Score = 27.9 bits (59), Expect = 9.3
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = +1
Query: 367 IXDGSGSTCEFLXLKHASRSXPHNNFSLW 453
+ DG+G C L LK P NF +W
Sbjct: 176 LGDGAGDYCPSLGLKAEDYMMPRKNFPVW 204
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,199,188
Number of Sequences: 28952
Number of extensions: 411379
Number of successful extensions: 1268
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1266
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2038669600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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