Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_pT_D09
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 91 1e-19
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 82 7e-17
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 64 2e-11
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 52 6e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 46 5e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 1.2
SPBC25H2.14 |mug16||UNC-50 family protein|Schizosaccharomyces po... 26 4.7
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 26 6.1
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 25 8.1
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 25 8.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 91.5 bits (217), Expect = 1e-19
Identities = 50/134 (37%), Positives = 77/134 (57%), Gaps = 6/134 (4%)
Frame = -2
Query: 680 SQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 513
+ +Q+AEF+EAF D D+DG I N+L SLG+ + EL +M+ E +G I+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 512 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDE 339
FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G++ S +EV +
Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125
Query: 338 AYDQMDIDDKGYID 297
+ D D G I+
Sbjct: 126 MIREADTDGDGVIN 139
Score = 28.7 bits (61), Expect = 0.87
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = -2
Query: 425 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 267
D G ID +E L D + +EV EA+ D D GYI +L +LT+
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 82.2 bits (194), Expect = 7e-17
Identities = 45/126 (35%), Positives = 65/126 (51%), Gaps = 2/126 (1%)
Frame = -2
Query: 713 KRTGSNVFXMFSQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG 534
KR S F + Q+ E KEAF +D D DG IG+ D++ SL + ASE ++ M
Sbjct: 32 KRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
Query: 533 EASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTWGDKF 360
+ PIN LT + + S +D ++ AF TFD + GKI +R AL + GD+
Sbjct: 92 SINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSMGDRM 150
Query: 359 SADEVD 342
EV+
Sbjct: 151 DPQEVE 156
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 64.1 bits (149), Expect = 2e-11
Identities = 39/140 (27%), Positives = 71/140 (50%), Gaps = 2/140 (1%)
Frame = -2
Query: 680 SQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 501
S++Q E KEAF D DKDG+I + + + SLG ++ EL ++ E I+ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 500 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQ 327
++ +N++ E++ I AF+ FD++ G I++ + + T G+K S +EV +
Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122
Query: 326 MDIDDKGYIDTTKLIAMLTA 267
D + G D + + A
Sbjct: 123 ADPTNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 52.4 bits (120), Expect = 6e-08
Identities = 35/142 (24%), Positives = 67/142 (47%), Gaps = 6/142 (4%)
Frame = -2
Query: 680 SQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 513
+++Q + EAF+ D DKD I ++LRA +LG A + E+ +++ + G +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 512 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDKFSADEVDE 339
+ + ++ D + + AF+ F DE GKI LR + E++
Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150
Query: 338 AYDQMDIDDKGYIDTTKLIAML 273
++ D+D G I+ + IA++
Sbjct: 151 MIEEFDLDQDGEINEQEFIAIM 172
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 46.0 bits (104), Expect = 5e-06
Identities = 33/136 (24%), Positives = 63/136 (46%), Gaps = 4/136 (2%)
Frame = -2
Query: 659 FKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 480
+K+AF D G I K + + G+ + E+ E+ ++ Q L + NR
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NR 66
Query: 479 MSGGS--DEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 312
+G + + + F+ FD++ G I LR+ L + G+K S +E+DE + + D
Sbjct: 67 PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD 126
Query: 311 KGYIDTTKLIAMLTAS 264
G ++ + M+ A+
Sbjct: 127 -GMVNYHDFVQMILAN 141
Score = 39.9 bits (89), Expect = 4e-04
Identities = 18/42 (42%), Positives = 25/42 (59%)
Frame = -2
Query: 662 EFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 537
EF + FQ D D G+IG +LR SLG S +E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 28.3 bits (60), Expect = 1.2
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -2
Query: 353 DEVDEAYDQMDIDDKGYID 297
+E +EA+D D+ KGYID
Sbjct: 12 EEAEEAFDLFDVTHKGYID 30
>SPBC25H2.14 |mug16||UNC-50 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 235
Score = 26.2 bits (55), Expect = 4.7
Identities = 8/20 (40%), Positives = 15/20 (75%)
Frame = -1
Query: 120 FYIHNNLLFPTFIYLYIMKI 61
F +H N FP+F+ LY++++
Sbjct: 135 FDVHCNSFFPSFVLLYVIQL 154
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.8 bits (54), Expect = 6.1
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -2
Query: 671 QVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASE 558
+ A+FK+ F DKD + + +L A + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.4 bits (53), Expect = 8.1
Identities = 9/26 (34%), Positives = 19/26 (73%)
Frame = +1
Query: 529 ASPTISSSSFSDASLPRESKVARKSF 606
+SP++ +++ + P++S V+RKSF
Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSF 60
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 25.4 bits (53), Expect = 8.1
Identities = 21/49 (42%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = +1
Query: 463 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKS 603
S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS 590
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,378,582
Number of Sequences: 5004
Number of extensions: 42158
Number of successful extensions: 175
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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