Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_pT_B11
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 107 1e-24
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 63 3e-11
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 58 8e-10
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 44 1e-05
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|... 28 0.95
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 1.7
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 27 2.2
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 5.1
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos... 25 6.7
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 8.9
SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyce... 25 8.9
>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 199
Score = 107 bits (257), Expect = 1e-24
Identities = 56/141 (39%), Positives = 82/141 (58%)
Frame = -1
Query: 516 IXGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSG 337
I P G ++H+ S Y+F+ GKY YCF N K V+FN+ G S
Sbjct: 62 IYNPAGQIMHQEVPNSMAQYSFTVKNPGKYMYCFYNDALDGESKEVLFNVH-GVIYI-SD 119
Query: 336 NEXEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVL 157
+ +A+ N L +++L T+ +KH+QEY R+RIHR+ ESTN RV WSI + +L
Sbjct: 120 EDLDAN-NPLLGKVRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVIL 178
Query: 156 LVMTIGQVYYLKRFFEVQRVV 94
+ + + Q++YLKR FEV+RVV
Sbjct: 179 VSVCVFQIFYLKRLFEVKRVV 199
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 62.9 bits (146), Expect = 3e-11
Identities = 33/140 (23%), Positives = 67/140 (47%), Gaps = 4/140 (2%)
Frame = -1
Query: 516 IXGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNL----EIGEPQ 349
I P + ++ F+ G+Y +CF N MS+ T K+V + E+ P
Sbjct: 64 IKAPSQKTVALGKKRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPA 123
Query: 348 TKSGNEXEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFE 169
+ + ++ + E++T L I Q Y + R+ + S +ST +R+ +S+ E
Sbjct: 124 LTRDEAKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAE 183
Query: 168 ASVLLVMTIGQVYYLKRFFE 109
+ +++ ++ QV+ +K FF+
Sbjct: 184 SIMVVALSALQVFIVKTFFK 203
>SPBC16E9.09c |||COPII vesicle coat component
Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 58.0 bits (134), Expect = 8e-10
Identities = 38/140 (27%), Positives = 64/140 (45%), Gaps = 3/140 (2%)
Frame = -1
Query: 504 DGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGN--- 334
+ ++I+K + S+ F+ G + C+ + L + N
Sbjct: 76 NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135
Query: 333 EXEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLL 154
E + L D + L T L +I +Q + R+ R +ES NSRVV W+I + VLL
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVVLL 195
Query: 153 VMTIGQVYYLKRFFEVQRVV 94
V I Q+ +L+RFF +++V
Sbjct: 196 VTCIWQLSHLQRFFVKEKLV 215
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 44.4 bits (100), Expect = 1e-05
Identities = 27/78 (34%), Positives = 41/78 (52%)
Frame = -1
Query: 327 EADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVM 148
EAD + D I+E IK Y+Q R+ R+ NESTN RV ++ L V+
Sbjct: 145 EADIRRARDFIEE-------IKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVL 197
Query: 147 TIGQVYYLKRFFEVQRVV 94
I Q+ YL+ FF+ + ++
Sbjct: 198 VIWQILYLRSFFQRKHLI 215
>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
Mts4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.9 bits (59), Expect = 0.95
Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 3/48 (6%)
Frame = +2
Query: 140 PIVMTSSTEASNID---HITTREFVLSFIERWIRSRTCIYS*SCLIVL 274
P +T + EA ID + E V+ F+E SR C+Y SC+ +L
Sbjct: 195 PFFLTHNAEADAIDLLQELGAIEKVVPFVELDNASRVCLYITSCVNLL 242
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 27.1 bits (57), Expect = 1.7
Identities = 13/29 (44%), Positives = 21/29 (72%), Gaps = 2/29 (6%)
Frame = -1
Query: 192 VVMWSIFEASVLLVM-TIGQVYYL-KRFF 112
+V+W +F+ VLLV T+G ++L K+FF
Sbjct: 560 LVLWMLFQVLVLLVQDTLGPRFFLPKKFF 588
>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.6 bits (56), Expect = 2.2
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = -2
Query: 506 LMATSFIKQRGSHLVFIHFLLQLQGSILIASVIKCHP 396
+M T +K G + +HF+ + G+ L ++ KC P
Sbjct: 170 VMVTEALKPYGQENLELHFVSNIDGTHLAEALKKCDP 206
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 25.4 bits (53), Expect = 5.1
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -1
Query: 321 DHNKLEDMIKELATTLRTIK 262
DH+KL+D +++ A+TL IK
Sbjct: 723 DHSKLDDDLEKFASTLDPIK 742
>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 449
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -3
Query: 232 SYPSFNK*KYKFTCSDVVYI*GFSTACH 149
SYP+F + +Y F DV Y +S CH
Sbjct: 361 SYPAFLRMRYNFNQDDVDYQ-LYSVVCH 387
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 24.6 bits (51), Expect = 8.9
Identities = 14/54 (25%), Positives = 28/54 (51%), Gaps = 4/54 (7%)
Frame = -1
Query: 366 EIGEPQTKSGNEXEADHNKLEDMIKE----LATTLRTIKHDQEYMQVRDRIHRS 217
E G P + + +K+E ++K L +TLR ++D + Q+R I+++
Sbjct: 20 ESGHPISLDSKTPPPNDSKIESIVKAQILFLLSTLREDQYDTKLEQIRQLINKN 73
>SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 217
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/50 (20%), Positives = 21/50 (42%)
Frame = -1
Query: 243 QVRDRIHRSINESTNSRVVMWSIFEASVLLVMTIGQVYYLKRFFEVQRVV 94
Q+R+ H+SI E + W ++ +G+ F+ ++V
Sbjct: 87 QLRNEEHKSIEEERRRLMAYWCVYGCVTAAESILGRFLSWVPFYSTSKIV 136
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,070,381
Number of Sequences: 5004
Number of extensions: 39242
Number of successful extensions: 110
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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