Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_pT_A04
(804 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot... 290 6e-79
At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ... 276 1e-74
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S... 219 2e-57
At5g16290.2 68418.m01904 acetolactate synthase small subunit, pu... 33 0.29
At5g16290.1 68418.m01903 acetolactate synthase small subunit, pu... 33 0.29
At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 30 1.6
At5g10470.1 68418.m01213 kinesin motor protein-related TH65 prot... 30 2.1
At4g37290.1 68417.m05277 expressed protein 29 4.8
At3g59870.1 68416.m06681 expressed protein hypothetical protein ... 29 4.8
At3g58400.1 68416.m06509 meprin and TRAF homology domain-contain... 28 6.3
At3g20760.1 68416.m02626 hypothetical protein 28 8.3
>At1g74030.1 68414.m08573 enolase, putative similar to
Swiss-Prot:P15007 enolase (EC 4.2.1.11)
(2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
hydro-lyase) [Drosophila melanogaster]
Length = 477
Score = 290 bits (712), Expect = 6e-79
Identities = 139/193 (72%), Positives = 162/193 (83%), Gaps = 1/193 (0%)
Frame = -1
Query: 804 YAGKIDIGMDVAAXEFF-KDGKYDLDFKNPDSNPGDYLSSEKLADVYLDFIKDFPMVSIE 628
Y GKI IGMDVAA EFF KDG+YDL+FK ++ LS+E LAD+Y +FIKDFP+VSIE
Sbjct: 281 YTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIE 340
Query: 627 DPFDQDDWSAWANLTGRTPIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGSVTES 448
DPFDQDDWS+WA+L IQ+VGDDL VTNPKRIA A++K++CN LLLKVNQIG+VTES
Sbjct: 341 DPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTES 400
Query: 447 IDAHLLAKKNGWGTMVSHRSGETEDTFIADLVVGLSTGQIKTGAPCRSERLAKYNQILRI 268
I A L +K GWG MVSHRSGETED FIADL VGL++GQIKTGAPCRSERL+KYNQ+LRI
Sbjct: 401 IQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRI 460
Query: 267 EEELGVNAKYAGK 229
EEELG N +YAG+
Sbjct: 461 EEELG-NVRYAGE 472
>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
enolase (EC 4.2.1.11) (2-phosphoglycerate
dehydratase)(2-phospho-D- glycerate hydro-lyase)
[Arabidopsis thaliana]
Length = 444
Score = 276 bits (676), Expect = 1e-74
Identities = 132/196 (67%), Positives = 156/196 (79%), Gaps = 3/196 (1%)
Frame = -1
Query: 804 YAGKIDIGMDVAAXEFFKDGK-YDLDFKNPDSNPGDYLSSEKLADVYLDFIKDFPMVSIE 628
Y GK+ IGMDVAA EF+ + K YDL+FK ++N +S + L D+Y F+ ++P+VSIE
Sbjct: 241 YTGKVVIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIE 300
Query: 627 DPFDQDDWSAWANLTGR--TPIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGSVT 454
DPFDQDDW +A +T T +QIVGDDL VTNPKR+A A+ +K+CN LLLKVNQIGSVT
Sbjct: 301 DPFDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVT 360
Query: 453 ESIDAHLLAKKNGWGTMVSHRSGETEDTFIADLVVGLSTGQIKTGAPCRSERLAKYNQIL 274
ESI+A ++KK GWG M SHRSGETEDTFIADL VGLSTGQIKTGAPCRSERLAKYNQ+L
Sbjct: 361 ESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLL 420
Query: 273 RIEEELGVNAKYAGKN 226
RIEEELG A YAG N
Sbjct: 421 RIEEELGSEAIYAGVN 436
>At2g29560.1 68415.m03590 enolase, putative similar to enolase
[Spinacia oleracea] gi|8919731|emb|CAB96173
Length = 475
Score = 219 bits (534), Expect = 2e-57
Identities = 101/193 (52%), Positives = 137/193 (70%)
Frame = -1
Query: 804 YAGKIDIGMDVAAXEFFKDGKYDLDFKNPDSNPGDYLSSEKLADVYLDFIKDFPMVSIED 625
Y KI I +D+AA F KYDLD K+P+ + ++ S+E + D+Y + D+P+VSIED
Sbjct: 278 YNDKIKIAIDIAATNFCLGTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIED 337
Query: 624 PFDQDDWSAWANLTGRTPIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGSVTESI 445
PFD++DW + Q+VGDDL ++N KR+ A+++ +CN LLLKVNQIG+VTE+I
Sbjct: 338 PFDKEDWEHTKYFSSLGICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAI 397
Query: 444 DAHLLAKKNGWGTMVSHRSGETEDTFIADLVVGLSTGQIKTGAPCRSERLAKYNQILRIE 265
+ +A+ WG + SHR GETED+FI+DL VGL+TG IK GAPCR ER KYNQ+LRIE
Sbjct: 398 EVVKMARDAQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIE 457
Query: 264 EELGVNAKYAGKN 226
EELG A YAG++
Sbjct: 458 EELGDQAVYAGED 470
>At5g16290.2 68418.m01904 acetolactate synthase small subunit,
putative similar to gi:5931761 from Nicotiana
plumbaginifolia
Length = 477
Score = 32.7 bits (71), Expect = 0.29
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +1
Query: 502 LLLNCSSDTLRVCHRQIITNNLNRRASGEVCPCRPIILVKRILNGHHGKIFDE 660
L+ S +T+ + +T N N + G+V P P K +L+ H G ++DE
Sbjct: 248 LVKESSHETVAEKTKLALTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300
>At5g16290.1 68418.m01903 acetolactate synthase small subunit,
putative similar to gi:5931761 from Nicotiana
plumbaginifolia
Length = 477
Score = 32.7 bits (71), Expect = 0.29
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +1
Query: 502 LLLNCSSDTLRVCHRQIITNNLNRRASGEVCPCRPIILVKRILNGHHGKIFDE 660
L+ S +T+ + +T N N + G+V P P K +L+ H G ++DE
Sbjct: 248 LVKESSHETVAEKTKLALTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300
>At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain,
PF04937: Protein of unknown function (DUF 659)
Length = 433
Score = 30.3 bits (65), Expect = 1.6
Identities = 19/40 (47%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
Frame = +2
Query: 242 LALTPSSSSMRRIWLYLARRSER----HGAPVLI*PVDKP 349
L+LTPSSSS R W SER +G PV P +KP
Sbjct: 358 LSLTPSSSSFERNWSAFEGPSERDEEPNGEPVYQTPNEKP 397
>At5g10470.1 68418.m01213 kinesin motor protein-related TH65
protein, Arabidopsis thaliana, EMBL:AJ001729; contains
Pfam profile PF00225: Kinesin motor domain
Length = 1273
Score = 29.9 bits (64), Expect = 2.1
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = -2
Query: 269 LKRNLVSTPNTPGRTSVDRSKNSLKKNVK 183
L+RN +STP+ P + ++ N LK+ VK
Sbjct: 42 LRRNSISTPSLPPKQAIASKVNGLKEKVK 70
>At4g37290.1 68417.m05277 expressed protein
Length = 84
Score = 28.7 bits (61), Expect = 4.8
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -1
Query: 384 ETEDTFIADLVVGLSTGQIKTGAPCRSE 301
+TE+ F+A L GLS G IK P E
Sbjct: 31 KTEEKFVASLFDGLSLGSIKDSGPSPGE 58
>At3g59870.1 68416.m06681 expressed protein hypothetical protein
F6E13.7 - Arabidopsis thaliana, PIR:T00674
Length = 288
Score = 28.7 bits (61), Expect = 4.8
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -1
Query: 645 PMVSIEDPFDQDDWSAWANLTGRTPIQIVGDDLTVTNPKRIATAVE 508
PM E DQDD +W N + D+ T T RI T +
Sbjct: 66 PMCFPEQTRDQDDVDSWVNKEDNVTCRFDSDEDTTTTGLRIPTQAQ 111
>At3g58400.1 68416.m06509 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam PF00917: MATH domain
Length = 316
Score = 28.3 bits (60), Expect = 6.3
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = -1
Query: 744 KYDLDFKNPDSNPGDYLSSEKLADVYLDFIKDFPMVSIEDPF 619
+YD D+ PD+ DY + E+ ++ D+ D P I F
Sbjct: 13 EYDSDYSYPDNYMSDYSAYEEESEPEFDYGDDSPFEGIPASF 54
>At3g20760.1 68416.m02626 hypothetical protein
Length = 373
Score = 27.9 bits (59), Expect = 8.3
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +3
Query: 30 NITVKINNLIHSTRTFTTVLKNMIGIAYLVQ 122
N VKI NL+ + ++F +NM +++LV+
Sbjct: 247 NKRVKIENLVLNRKSFAQTAENMFALSFLVK 277
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,604,016
Number of Sequences: 28952
Number of extensions: 377528
Number of successful extensions: 918
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 893
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 915
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1824072800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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