BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P02_F_P10 (708 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g07390.1 68417.m01134 PQ-loop repeat family protein / transme... 123 1e-28 At5g59470.1 68418.m07453 PQ-loop repeat family protein / transme... 109 1e-24 At3g50580.1 68416.m05532 proline-rich family protein contains pr... 30 1.7 At3g44830.1 68416.m04830 lecithin:cholesterol acyltransferase fa... 28 5.3 At2g39550.1 68415.m04852 geranylgeranyl transferase type I beta ... 28 7.0 At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9... 27 9.2 At1g62935.1 68414.m07106 hypothetical protein 27 9.2 >At4g07390.1 68417.m01134 PQ-loop repeat family protein / transmembrane family protein similar to SP|Q60441 Mannose-P-dolichol utilization defect 1 protein (Suppressor of Lec15 and Lec35 glycosylation mutation) {Cricetulus griseus}, Lec35 protein [Cricetulus griseus] GI:9858721; contains Pfam profile PF04193: PQ loop repeat Length = 235 Score = 123 bits (296), Expect = 1e-28 Identities = 63/170 (37%), Positives = 97/170 (57%), Gaps = 1/170 (0%) Frame = +3 Query: 177 PQIFKILQSKSAEGINIYGVYLELFAITANFAYSYVMGFPFSAWGEGTFLAIQTAMIAAL 356 PQI KI+Q KS G+++ LE+ T + AY G PFSA+GE FL IQ ++ A Sbjct: 48 PQIMKIVQHKSVRGLSVVAFELEVVGYTISLAYCLHKGLPFSAFGEMAFLLIQALILVAC 107 Query: 357 VLHYGG-APMKGGIFLSVYCAIVSVLVSGYTSTDILWTMQAVTVPIILIAKSIQIGTNYK 533 + +Y P+ I +YCA+ +++G + + + A I L A+ QI N+K Sbjct: 108 IYYYSQPVPVTTWIRPLLYCAVAPTVLAGQINPTLFEALYASQHAIFLFARLPQIWKNFK 167 Query: 534 NGSTGQLSFITCFLLFGGSVXRIFTSIQETGDSIIILTYCVSTIANGAIV 683 N STG+LSF+T F+ F GS+ R+FTS+QE I+ + + + NG+I+ Sbjct: 168 NKSTGELSFLTFFMNFAGSIVRVFTSLQEKAPISILTGFALGVVTNGSIL 217 >At5g59470.1 68418.m07453 PQ-loop repeat family protein / transmembrane family protein similar to SP|Q60441 Mannose-P-dolichol utilization defect 1 protein (Suppressor of Lec15 and Lec35 glycosylation mutation) (SL15) {Cricetulus griseus}; contains Pfam profile PF04193: PQ loop repeat Length = 239 Score = 109 bits (263), Expect = 1e-24 Identities = 63/171 (36%), Positives = 95/171 (55%), Gaps = 2/171 (1%) Frame = +3 Query: 177 PQIFKILQSKSAEGINIYGVYLELFAITANFAYSYVMGFPFSAWGEGTFLAIQTAMIAAL 356 PQI KI+ +KS +G+++ LE+ T + AY PFSA+GE FL IQ A+I Sbjct: 48 PQIMKIVDNKSVKGLSVVAFELEVIGYTISLAYCLNKDLPFSAFGELAFLLIQ-ALILVA 106 Query: 357 VLHYGGAPMKGGIFLS--VYCAIVSVLVSGYTSTDILWTMQAVTVPIILIAKSIQIGTNY 530 ++Y P+ ++ +Y AI + +G + + A I L A+ QI N+ Sbjct: 107 CIYYFSQPLSVTTWVKAILYFAIAPTVFAGKIDPFLFEALYASKHLIFLSARIPQIWKNF 166 Query: 531 KNGSTGQLSFITCFLLFGGSVXRIFTSIQETGDSIIILTYCVSTIANGAIV 683 +N STGQLSF+TC + FGG++ R+FTSIQE ++L +S NG I+ Sbjct: 167 RNKSTGQLSFLTCLMNFGGALARVFTSIQEKAPLSMLLGIVLSIFTNGIIM 217 >At3g50580.1 68416.m05532 proline-rich family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 265 Score = 29.9 bits (64), Expect = 1.7 Identities = 18/57 (31%), Positives = 26/57 (45%) Frame = +2 Query: 485 SYHSHSQVNPNRNELQKWQHWSTVVYNLLPVVWR*CXKNLHINSRNWRLHYYSDLLC 655 S+HS S NP ++ W+H + N+ PV C + + S RL SD C Sbjct: 162 SHHSSSPSNPPHHQQNPWEHIERCMINMGPVGM--CRMQMEV-SFYTRLFQVSDYCC 215 >At3g44830.1 68416.m04830 lecithin:cholesterol acyltransferase family protein / LACT family protein similar to lecithin:cholesterol acyltransferase [Rattus norvegicus] GI:2306762; contains Pfam profile PF02450: Lecithin:cholesterol acyltransferase (phosphatidylcholine-sterol acyltransferase) Length = 665 Score = 28.3 bits (60), Expect = 5.3 Identities = 11/33 (33%), Positives = 21/33 (63%) Frame = +2 Query: 545 WSTVVYNLLPVVWR*CXKNLHINSRNWRLHYYS 643 W+ ++ NL + + KNLH+ S +WRL +++ Sbjct: 174 WAVLIENLAKIGYE--GKNLHMASYDWRLSFHN 204 >At2g39550.1 68415.m04852 geranylgeranyl transferase type I beta subunit (GGT-IB) nearly identical to GI:11878247 Length = 375 Score = 27.9 bits (59), Expect = 7.0 Identities = 22/81 (27%), Positives = 40/81 (49%), Gaps = 4/81 (4%) Frame = +3 Query: 372 GAPMKGGIFLSVYCAIVSVLVSGYTSTDILW--TMQAVTVPIILIAKSIQIGTNYKNGST 545 G+ GG + YCAI S+ + GY D+L + ++ P +L+ +Q N G Sbjct: 228 GSESHGG---ATYCAIASLRLMGYIGVDLLSNDSSSSIIDPSLLLNWCLQRQAN-DGGFQ 283 Query: 546 GQLS--FITCFLLFGGSVXRI 602 G+ + TC+ + G+V ++ Sbjct: 284 GRTNKPSDTCYAFWIGAVLKL 304 >At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q96CW5 Gamma-tubulin complex component 3 {Homo sapiens} Length = 838 Score = 27.5 bits (58), Expect = 9.2 Identities = 14/43 (32%), Positives = 24/43 (55%) Frame = +1 Query: 184 FSKFYKVRVPKESIFMEFIWSCLLSLRTLLIAM*WVSHSVHGV 312 FS Y+ RVP +++F E + S L + L + V H++ G+ Sbjct: 580 FSLEYEARVPLDTVFTESVLSKYLRVFNFLWKLKRVEHALIGI 622 >At1g62935.1 68414.m07106 hypothetical protein Length = 176 Score = 27.5 bits (58), Expect = 9.2 Identities = 14/46 (30%), Positives = 24/46 (52%) Frame = +3 Query: 552 LSFITCFLLFGGSVXRIFTSIQETGDSIIILTYCVSTIANGAIVYK 689 L F C +LF + R + +ETGD ++ T+ + +G I+ K Sbjct: 35 LFFFLCTILFLLIIDRNRSVYEETGDFYVLYTFQEDNMLDGEIIDK 80 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,637,347 Number of Sequences: 28952 Number of extensions: 335907 Number of successful extensions: 765 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 748 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 763 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1526202912 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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