Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_O22
(515 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 151 4e-37
At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi... 150 5e-37
At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) 148 2e-36
At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 31 0.61
At5g63900.1 68418.m08023 PHD finger family protein contains Pfam... 30 0.80
At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) simila... 30 1.1
At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) simila... 30 1.1
At4g34630.1 68417.m04918 expressed protein 29 1.9
At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-... 29 1.9
At1g66070.1 68414.m07499 translation initiation factor-related s... 29 1.9
At5g39410.1 68418.m04774 expressed protein 28 3.2
At5g12000.1 68418.m01403 protein kinase family protein contains ... 28 4.3
At3g63310.1 68416.m07121 expressed protein low similarity to N-m... 28 4.3
At1g50820.1 68414.m05715 hypothetical protein 27 7.5
At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive eff... 27 9.9
At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive eff... 27 9.9
At4g02090.1 68417.m00280 expressed protein 27 9.9
At1g14020.1 68414.m01656 expressed protein contains Pfam PF03138... 27 9.9
>At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A)
Length = 135
Score = 151 bits (365), Expect = 4e-37
Identities = 66/135 (48%), Positives = 93/135 (68%), Gaps = 1/135 (0%)
Frame = +1
Query: 49 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 228
M K MKPGK V++L GRY G+KA++VK++D+GT +K YGH VAG+ +YP KV ++
Sbjct: 1 MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 229 KIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK-FSAKDLKDPAKRKKLRFNTRVRFEER 405
K K+S++K F KV+NY H+MPTRYT+D + SA + K+ + +FEER
Sbjct: 61 KTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEER 120
Query: 406 YKSGKNKWFFQKLRF 450
+K+GKN+WFF KLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135
>At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar
to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum
tuberosum]
Length = 135
Score = 150 bits (364), Expect = 5e-37
Identities = 65/135 (48%), Positives = 95/135 (70%), Gaps = 1/135 (0%)
Frame = +1
Query: 49 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 228
M K +K K V++L GRYAG+KA+++K++D+GTSD+ YGH VAG+ +YP KV ++
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 229 KIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEER 405
K K+S++K F+K+VNY HLMPTRYT+D ++ + D LK K+ + + EER
Sbjct: 61 KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEER 120
Query: 406 YKSGKNKWFFQKLRF 450
+K+GKN+WFF KLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135
>At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)
Length = 135
Score = 148 bits (359), Expect = 2e-36
Identities = 63/135 (46%), Positives = 94/135 (69%), Gaps = 1/135 (0%)
Frame = +1
Query: 49 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 228
M K +K K V++L GRYAG+KA+++K++D+G D+PYGH VAG+ +YP KV ++
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 229 KIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEER 405
K K+S++K F+K+VNY HLMPTRYT+D ++ + D L+ K+ + + EER
Sbjct: 61 KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEER 120
Query: 406 YKSGKNKWFFQKLRF 450
+K+GKN+WFF KLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135
>At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar
to 60S ribosomal protein L6 GI:7208784 from [Cicer
arietinum]
Length = 233
Score = 30.7 bits (66), Expect = 0.61
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +1
Query: 40 PPSMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEG 144
P + + PG V+++L+GR+ G++ + +K G
Sbjct: 82 PTKLKASITPGTVLIILAGRFKGKRVVFLKQLSSG 116
>At5g63900.1 68418.m08023 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 557
Score = 30.3 bits (65), Expect = 0.80
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 376 FNTRVRFEERYKSGKNKWFF 435
+N R + E+RYKS K KWF+
Sbjct: 58 YNKRNKKEQRYKSPKGKWFY 77
>At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 29.9 bits (64), Expect = 1.1
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +1
Query: 40 PPSMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEG 144
P + + PG V+++L+GR+ G++ + +K G
Sbjct: 82 PAKLRASITPGTVLIILAGRFKGKRVVFLKQLASG 116
>At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 29.9 bits (64), Expect = 1.1
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +1
Query: 40 PPSMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEG 144
P + + PG V+++L+GR+ G++ + +K G
Sbjct: 82 PTKLRASITPGTVLIILAGRFKGKRVVFLKQLASG 116
>At4g34630.1 68417.m04918 expressed protein
Length = 199
Score = 29.1 bits (62), Expect = 1.9
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +1
Query: 136 DEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSK 249
D+G D+ Y ++ + PR V K++G+ ++ K K
Sbjct: 130 DDGEEDREYDDSYDLDEELVPRSVSKKVGRQRMRKLGK 167
>At1g72440.1 68414.m08377 CCAAT-box-binding transcription
factor-related similar to CCAAT-box-binding transcription
factor (CCAAT-binding factor) (CBF) (Swiss-Prot:Q03701)
[Homo sapiens], GB:P53569 [Mus musculus]
Length = 1056
Score = 29.1 bits (62), Expect = 1.9
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = +1
Query: 214 RMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKK 369
R K K KR + PF + Y HL+ D K K +P K+KK
Sbjct: 1000 RSKKKKKEKRKRKSPFASLEEYKHLIDQDEKED---SKTKRKATSEPTKKKK 1048
>At1g66070.1 68414.m07499 translation initiation factor-related
similar to Eukaryotic translation initiation factor 3
subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
(Swiss-Prot:O75822) [Homo sapiens]
Length = 226
Score = 29.1 bits (62), Expect = 1.9
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = +1
Query: 247 KIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNK 426
+IKP+ K +Y L+ T + S A D+KD A N +++ E+ +GK K
Sbjct: 139 RIKPYEKSYHYIALLKT--IMRLSLTNMKAADVKDVASSITTIANEKLKAEKEAAAGKKK 196
>At5g39410.1 68418.m04774 expressed protein
Length = 454
Score = 28.3 bits (60), Expect = 3.2
Identities = 15/59 (25%), Positives = 31/59 (52%)
Frame = +1
Query: 40 PPSMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKR 216
PP+ G ++ K + + + + A+VV+ ++KP+G + GI+ P ++ KR
Sbjct: 229 PPAKGPTLENQKTIGLWALKLPSADAVVVRRTLTTLTEKPHG---LPGINESPEQIQKR 284
>At5g12000.1 68418.m01403 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 703
Score = 27.9 bits (59), Expect = 4.3
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +1
Query: 187 DRYPRKVHKRMGKNKIHKR-SKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKDLKDPAKR 363
DR PR +R G+N + +R S K V H +PT ++DF++E K ++ R
Sbjct: 188 DRSPRS--QRNGRNTVPERYSHENKGFKPVREMHKIPTNGSLDFNYEFRQGKGQRNSTGR 245
>At3g63310.1 68416.m07121 expressed protein low similarity to
N-methyl-D-aspartate receptor-associated protein
[Drosophila melanogaster] GI:567104; contains Pfam
profile PF01027: Uncharacterized protein family UPF0005
Length = 239
Score = 27.9 bits (59), Expect = 4.3
Identities = 25/74 (33%), Positives = 36/74 (48%), Gaps = 1/74 (1%)
Frame = -1
Query: 410 LYLSSKRTRVLKRSFLRFAGSFRSFALNFS-KLKSTV*RVGIK*L*FTTLTKGFILDLLW 234
LY + L+ SF+R S S L + + +TV +V + FTT T GF L +L
Sbjct: 15 LYPMMSESPELRWSFIRKVYSIISIQLLVTIAVAATVVKVHSISVFFTTTTAGFALYILL 74
Query: 233 ILFFPILLCTFRGY 192
IL I++C Y
Sbjct: 75 ILTPLIVMCPLYYY 88
>At1g50820.1 68414.m05715 hypothetical protein
Length = 528
Score = 27.1 bits (57), Expect = 7.5
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 123 SQELRRRYLRQAIRACLRRWYRQVPPESAQED 218
S RR+Y++QA R + +QVP ++ ++D
Sbjct: 476 STNKRRKYMKQAHRGTMGLCQKQVPSDNDEDD 507
>At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 676
Score = 26.6 bits (56), Expect = 9.9
Identities = 16/60 (26%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +1
Query: 166 HAFVAGID-RYPRKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD 342
HA + G + + P VH M + RS + V + + HL+ +Y + S +F K+
Sbjct: 391 HAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLVLLGHNKHLLDVQYRMHPSISRFPNKE 450
>At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 839
Score = 26.6 bits (56), Expect = 9.9
Identities = 16/60 (26%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +1
Query: 166 HAFVAGID-RYPRKVHKRMGKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEKFSAKD 342
HA + G + + P VH M + RS + V + + HL+ +Y + S +F K+
Sbjct: 554 HAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLVLLGHNKHLLDVQYRMHPSISRFPNKE 613
>At4g02090.1 68417.m00280 expressed protein
Length = 202
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +1
Query: 10 DAMSTSKGNIPPSMGKIMKPGKVVLVLSGRYAGRKA 117
DA + + P K ++PGK+ LVL + +G +A
Sbjct: 61 DAKTVKRKLAPLPADKTLEPGKIYLVLPAKRSGGRA 96
>At1g14020.1 68414.m01656 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'growth regulator protein -related' based on similarity
to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana
tabacum], which, due to scienitific fraud was retracted.
Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908.
PMID:10400497.
Length = 499
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = -3
Query: 264 DERLYLGPLVDFILSHPLVHFPGVPVDTSDEGMPVWL 154
DE+ YL ++ H +VHF ++ G+P+ L
Sbjct: 186 DEKYYLKQVLPLFSKHKVVHFNRTDTRLANNGLPLSL 222
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,293,008
Number of Sequences: 28952
Number of extensions: 203997
Number of successful extensions: 672
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 662
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 669
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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