Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_M21
(666 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g02670.1 68416.m00258 proline-rich family protein contains pr... 30 0.041
At5g44090.1 68418.m05394 calcium-binding EF hand family protein,... 32 0.30
At3g05220.2 68416.m00570 heavy-metal-associated domain-containin... 29 2.1
At3g05220.1 68416.m00569 heavy-metal-associated domain-containin... 29 2.1
At2g37410.2 68415.m04588 mitochondrial import inner membrane tra... 29 2.1
At2g37410.1 68415.m04587 mitochondrial import inner membrane tra... 29 2.1
At4g21120.1 68417.m03054 amino acid permease family protein simi... 29 2.8
At1g03930.1 68414.m00378 protein kinase (ADK1) identical to dual... 29 2.8
At4g13350.2 68417.m02088 human Rev interacting-like protein-rela... 28 6.4
At4g13350.1 68417.m02087 human Rev interacting-like protein-rela... 28 6.4
At3g60160.1 68416.m06717 ABC transporter family protein similar ... 27 8.5
At1g32330.1 68414.m03983 heat shock transcription factor family ... 27 8.5
>At3g02670.1 68416.m00258 proline-rich family protein contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 217
Score = 30.3 bits (65), Expect = 1.2
Identities = 16/37 (43%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = +1
Query: 361 APGCCVPKCFAEK--GNRGFPGSPGPQGPRGLPGHEG 465
+PG +P F GN G PG PG G G+PG G
Sbjct: 94 SPGFRLPFPFPSSPGGNPGIPGIPGIPGLPGIPGSPG 130
Score = 29.5 bits (63), Expect = 2.1
Identities = 23/67 (34%), Positives = 25/67 (37%), Gaps = 1/67 (1%)
Frame = -2
Query: 653 PSVPLQPSLPGIPGLXXXXXXXXXXXXXXXXXXPVLPLSPLGPRGPCGPI*PFCPLGPCA 474
PS P S+PGIPG LP PL P G PF PL P
Sbjct: 138 PSSPGGGSIPGIPGSPGFRLPFPFPPSGGGIPGLPLPFPPLPPVTIPGLPLPFPPLPPVT 197
Query: 473 -PSAPSW 456
PS P +
Sbjct: 198 IPSFPGF 204
Score = 28.7 bits (61), Expect = 3.7
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1
Query: 589 GSPGDQGRPGIPGRDGCNGTDG 654
G+PG G PGIPG G G+ G
Sbjct: 109 GNPGIPGIPGIPGLPGIPGSPG 130
Score = 28.3 bits (60), Expect(2) = 0.041
Identities = 22/63 (34%), Positives = 28/63 (44%), Gaps = 9/63 (14%)
Frame = +1
Query: 304 VYDPDTRQRTSTAINRNCTAPGCCVPKCFAEK--GNRGFPGSPG-------PQGPRGLPG 456
V DP + ++S + +PG +P F GN G PGSPG P P G PG
Sbjct: 53 VSDPFSSLQSSPPTSPIPGSPGFRLPFPFPSSPGGNPGIPGSPGFRLPFPFPSSPGGNPG 112
Query: 457 HEG 465
G
Sbjct: 113 IPG 115
Score = 25.8 bits (54), Expect(2) = 0.041
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = +1
Query: 583 IQGSPGDQGRPGIPGRDG 636
I G PG G PGIPG G
Sbjct: 113 IPGIPGIPGLPGIPGSPG 130
>At5g44090.1 68418.m05394 calcium-binding EF hand family protein,
putative / protein phosphatase 2A 62 kDa B'' regulatory
subunit, putative contains Pfam profile: PF00036 EF
hand; identical to cDNA protein phosphatase 2A 62 kDa
B'' regulatory subunit GI:5533378
Length = 538
Score = 32.3 bits (70), Expect = 0.30
Identities = 20/58 (34%), Positives = 27/58 (46%), Gaps = 9/58 (15%)
Frame = +1
Query: 280 PELGQNFAVYDPDTRQRTSTAINRNCT----APGCCVPKCFAEKGN-----RGFPGSP 426
PE G+ DT+ T ++++NCT A G +P F G RG PGSP
Sbjct: 45 PETGRLVKSLIDDTKSSTPVSVSKNCTSLNVACGSALPSVFLNSGTPPLSPRGSPGSP 102
>At3g05220.2 68416.m00570 heavy-metal-associated domain-containing
protein similar to farnesylated protein 1 (GI:23304411)
{Hordeum vulgare subsp. spontaneum}; contains Pfam
profile PF00403: Heavy-metal-associated domain
Length = 478
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -2
Query: 545 PLSPLGPRGPCGPI*PFCPLGPCAPSAP 462
P+ P GP GP GP+ P+G P P
Sbjct: 326 PMGPGGPMGPGGPMGQGGPMGMMGPGGP 353
>At3g05220.1 68416.m00569 heavy-metal-associated domain-containing
protein similar to farnesylated protein 1 (GI:23304411)
{Hordeum vulgare subsp. spontaneum}; contains Pfam
profile PF00403: Heavy-metal-associated domain
Length = 577
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -2
Query: 545 PLSPLGPRGPCGPI*PFCPLGPCAPSAP 462
P+ P GP GP GP+ P+G P P
Sbjct: 425 PMGPGGPMGPGGPMGQGGPMGMMGPGGP 452
>At2g37410.2 68415.m04588 mitochondrial import inner membrane
translocase (TIM17) nearly identical to SP|Q9SP35
Mitochondrial import inner membrane translocase subunit
TIM17 {Arabidopsis thaliana}
Length = 243
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 583 IQGSPGDQGRPGIPGRDGCNGTDG 654
+QG PG QG G+PG G G G
Sbjct: 150 MQGMPGMQGMQGMPGMPGMQGMPG 173
Score = 28.7 bits (61), Expect = 3.7
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 394 EKGNRGFPGSPGPQGPRGLPGHEG 465
+ G +G PG G QG G+PG +G
Sbjct: 147 DPGMQGMPGMQGMQGMPGMPGMQG 170
Score = 27.5 bits (58), Expect = 8.5
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +1
Query: 400 GNRGFPGSPGPQGPRGLPGHEGAE 471
G +G G PG G +G+PG +G +
Sbjct: 155 GMQGMQGMPGMPGMQGMPGMQGMQ 178
>At2g37410.1 68415.m04587 mitochondrial import inner membrane
translocase (TIM17) nearly identical to SP|Q9SP35
Mitochondrial import inner membrane translocase subunit
TIM17 {Arabidopsis thaliana}
Length = 243
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 583 IQGSPGDQGRPGIPGRDGCNGTDG 654
+QG PG QG G+PG G G G
Sbjct: 150 MQGMPGMQGMQGMPGMPGMQGMPG 173
Score = 28.7 bits (61), Expect = 3.7
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 394 EKGNRGFPGSPGPQGPRGLPGHEG 465
+ G +G PG G QG G+PG +G
Sbjct: 147 DPGMQGMPGMQGMQGMPGMPGMQG 170
Score = 27.5 bits (58), Expect = 8.5
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +1
Query: 400 GNRGFPGSPGPQGPRGLPGHEGAE 471
G +G G PG G +G+PG +G +
Sbjct: 155 GMQGMQGMPGMPGMQGMPGMQGMQ 178
>At4g21120.1 68417.m03054 amino acid permease family protein similar
to cationic amino acid transporter-1 [Rattus norvegicus]
GI:1589917; contains Pfam profile PF00324: Amino acid
permease
Length = 594
Score = 29.1 bits (62), Expect = 2.8
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = -3
Query: 274 VLIGVVYRFYYTTHIDLQRMVAPYLAPLHEAS*SAANSLEYMLFQSAYLS 125
+L+G + + Y THI M+ P+LA ++ + + N+ ML +A ++
Sbjct: 363 LLVGAIGQARYMTHIARAHMMPPWLAQVNAKTGTPINATVVMLAATALIA 412
>At1g03930.1 68414.m00378 protein kinase (ADK1) identical to dual
specificity kinase 1 (ADK1) [Arabidopsis thaliana]
gi|1216484|gb|AAB47968; supported by cDNA gi:18700076
and gi:1216483. Note: differences between cDNAs in the
11th exon, possibly due to errors or alternative
splicing.
Length = 471
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 202 DTERPYAVNQYGLYNRNDIPPQSRP 276
D+ERP + ++YG +R IP SRP
Sbjct: 378 DSERPRSSSRYGSSSRRAIPSSSRP 402
>At4g13350.2 68417.m02088 human Rev interacting-like protein-related
/ hRIP protein-related similar to SP|P52594
Nucleoporin-like protein RIP (HIV-1 Rev-binding protein)
(Rev interacting protein) (Rev/Rex activation
domain-binding protein) {Homo sapiens}; contains Pfam
profile PF01412: Putative GTPase activating protein for
Arf
Length = 602
Score = 27.9 bits (59), Expect = 6.4
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 6/72 (8%)
Frame = +1
Query: 142 GITYIPGNLPPTNWPREVAP----DTERPYAVNQYGL--YNRNDIPPQSRPEPELGQNFA 303
G + +P +L N+P AP P+A++ YG+ YN N +P Q+ P+P N
Sbjct: 431 GRSELPADLFAVNYPSYHAPVPGWQAGPPHAMH-YGMQQYN-NPVPYQNVPQPGKSMNPF 488
Query: 304 VYDPDTRQRTST 339
+ P +T T
Sbjct: 489 DFSPGPPSQTQT 500
>At4g13350.1 68417.m02087 human Rev interacting-like protein-related
/ hRIP protein-related similar to SP|P52594
Nucleoporin-like protein RIP (HIV-1 Rev-binding protein)
(Rev interacting protein) (Rev/Rex activation
domain-binding protein) {Homo sapiens}; contains Pfam
profile PF01412: Putative GTPase activating protein for
Arf
Length = 602
Score = 27.9 bits (59), Expect = 6.4
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 6/72 (8%)
Frame = +1
Query: 142 GITYIPGNLPPTNWPREVAP----DTERPYAVNQYGL--YNRNDIPPQSRPEPELGQNFA 303
G + +P +L N+P AP P+A++ YG+ YN N +P Q+ P+P N
Sbjct: 431 GRSELPADLFAVNYPSYHAPVPGWQAGPPHAMH-YGMQQYN-NPVPYQNVPQPGKSMNPF 488
Query: 304 VYDPDTRQRTST 339
+ P +T T
Sbjct: 489 DFSPGPPSQTQT 500
>At3g60160.1 68416.m06717 ABC transporter family protein similar to
ATP-binding cassette transporter MRP8 GI:18031899 from
[Arabidopsis thaliana]
Length = 1490
Score = 27.5 bits (58), Expect = 8.5
Identities = 16/53 (30%), Positives = 23/53 (43%)
Frame = -1
Query: 267 LGWYIVSIIQPILIYSVWSLRIWRHFTRPVSRRQIPWNICYSNPHIYHLASLL 109
LGW SIIQ + V S W+ + P +R + P ++H A L
Sbjct: 1052 LGWCAFSIIQIVGTIFVMSQVAWQRYYTPTARELSRMSGVERAPILHHFAESL 1104
>At1g32330.1 68414.m03983 heat shock transcription factor family
protein contains Pfam profile: PF00447 HSF-type
DNA-binding domain
Length = 485
Score = 27.5 bits (58), Expect = 8.5
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +1
Query: 244 NRNDIPPQSRPEPELGQNFAVYDPD 318
N ++ PP+S EP LG + + DPD
Sbjct: 376 NNSEKPPESFMEPNLGGSSPLLDPD 400
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,200,812
Number of Sequences: 28952
Number of extensions: 300544
Number of successful extensions: 925
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 910
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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