Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_M10
(815 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 75 1e-14
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 64 2e-11
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 37 0.003
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 27 2.4
SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosac... 27 4.2
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos... 27 4.2
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 26 5.6
SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su... 26 7.4
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 26 7.4
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 25 9.7
SPBC83.05 |||mitochondrial RNA-binding protein |Schizosaccharomy... 25 9.7
SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|... 25 9.7
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 25 9.7
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 74.9 bits (176), Expect = 1e-14
Identities = 53/140 (37%), Positives = 74/140 (52%), Gaps = 4/140 (2%)
Frame = +1
Query: 124 WISDVLGXPLPNGAYE--DVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMENIQRF-Q 294
WI + L L N + D L+ GVILC++ K A G+ + +E F MENI F
Sbjct: 12 WIEETLHTKL-NAQLDLLDQLQSGVILCRIC-KEALGANIRYKESNMPFVQMENISAFIN 69
Query: 295 AAIKKYGVPEEEIFQTADLFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKMADKNER 474
A + VP +++FQT+DLFERRN QV +++ R K LGPK+A+K R
Sbjct: 70 YAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLAEKKPR 129
Query: 475 TFTEEQLRAHNAELN-LQMG 531
F+ +Q R +N LQ G
Sbjct: 130 VFSAQQQREFREGVNSLQYG 149
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 64.5 bits (150), Expect = 2e-11
Identities = 34/94 (36%), Positives = 51/94 (54%), Gaps = 2/94 (2%)
Frame = +1
Query: 124 WISDVLGXPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QERGTNFQLMENIQRFQA 297
WI + LG L P +E LR+GV+L L K P + KI F+ +NI +F
Sbjct: 50 WIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLD 109
Query: 298 AIKKYGVPEEEIFQTADLFERRNIPQVTLCLYAL 399
I G+PE F+ D++E +N+P+V C++AL
Sbjct: 110 FIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 37.1 bits (82), Expect = 0.003
Identities = 27/82 (32%), Positives = 40/82 (48%), Gaps = 4/82 (4%)
Frame = +1
Query: 166 YEDVLRDGVILCKLANKLAPGSVKKIQERGTNFQLMEN----IQRFQAAIKKYGVPEEEI 333
+ D L +G +LC+LA K P + R +Q+ E + F + G+
Sbjct: 88 FVDALVNGKVLCQLAFKYYPKLASNWKPR---YQISERNTVYLNAFFHFLDFIGMFTPFR 144
Query: 334 FQTADLFERRNIPQVTLCLYAL 399
F+T DL R NIP+V CL+AL
Sbjct: 145 FETKDLVRRFNIPKVIYCLHAL 166
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 27.5 bits (58), Expect = 2.4
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -2
Query: 184 P*ARLRMRHLVAALPKHQRSK*EPLVTWSLXLAG 83
P R+ HL A+PK Q+ K +PL T S+ G
Sbjct: 785 PMTRVEKIHLEVAVPKSQKLKIQPLRTTSMEPGG 818
>SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase
Hrp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1373
Score = 26.6 bits (56), Expect = 4.2
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = +2
Query: 236 KKYKREGPTSSSWR--TFKGSKRQSRNTEYQKKK 331
+KY R+ PT SS R T GS ++ + KKK
Sbjct: 1215 RKYNRKAPTKSSTRQTTLDGSISNTKKSSRTKKK 1248
>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 26.6 bits (56), Expect = 4.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -1
Query: 275 SMSWKLVPLSCIFFTDPGANLLANLQRITPSLSTSSYAPFG 153
S+++KL + DP LAN +++ SLS +Y P G
Sbjct: 33 SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 26.2 bits (55), Expect = 5.6
Identities = 23/91 (25%), Positives = 34/91 (37%)
Frame = +1
Query: 31 GNPPNXLVXXPXFACTQCLPXTRTKXQXVLTWISDVLGXPLPNGAYEDVLRDGVILCKLA 210
G P + L+ T C T + V T V +P+ D L+ GV+ +
Sbjct: 174 GRPMSILLEKANATVTLCHSKTESIADIVRTADIVVAAIGIPHFVKADWLKKGVVAIDVG 233
Query: 211 NKLAPGSVKKIQERGTNFQLMENIQRFQAAI 303
P + KK R T EN + +AI
Sbjct: 234 INSIPDATKKSGYRLTGDIDFENAKEVASAI 264
>SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase
Sum3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 25.8 bits (54), Expect = 7.4
Identities = 14/52 (26%), Positives = 21/52 (40%)
Frame = +1
Query: 415 KHPEFTGPQLGPKMADKNERTFTEEQLRAHNAELNLQMGFNKGASQSGHGGF 570
+ F G G + + + R R N G++ G S SG+GGF
Sbjct: 565 RESSFGGNGRGGRYSGRGGRGGNAYGARDFRRPTNSSSGYSSGPSYSGYGGF 616
>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
Sad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 25.8 bits (54), Expect = 7.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 524 KWASIRAPPSPVMVASVTPATCKLLLNFSQYF 619
++ IR+PP+ V + S+TP NF Q +
Sbjct: 46 RYTKIRSPPTRVSIESITPKQRFPAPNFEQAY 77
>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 749
Score = 25.4 bits (53), Expect = 9.7
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = -1
Query: 212 LANLQRITPSLSTSSYAPFGSGXPKTSEIQ 123
L+N +TPS S S Y P +T +Q
Sbjct: 689 LSNFNSVTPSSSASLYPPLIPSEARTPSVQ 718
>SPBC83.05 |||mitochondrial RNA-binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 773
Score = 25.4 bits (53), Expect = 9.7
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = +1
Query: 616 FSTLISVFVHGRIWLNDFFTF 678
FST I VHG WL D + F
Sbjct: 140 FSTPIPHMVHGIPWLQDLYIF 160
>SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 623
Score = 25.4 bits (53), Expect = 9.7
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = -1
Query: 386 HNVTWGMLRLSKRSAVWN 333
HN WG R + +A+WN
Sbjct: 491 HNFVWGRFRELEHAAMWN 508
>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 535
Score = 25.4 bits (53), Expect = 9.7
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 306 LDCRLEPLNVLHELEVGPSLLYFFH 232
LD LEPL LHE + L+FF+
Sbjct: 238 LDAYLEPLVHLHEYSRRSNTLFFFY 262
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,460,778
Number of Sequences: 5004
Number of extensions: 77589
Number of successful extensions: 186
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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