BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P02_F_L19 (842 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_58242| Best HMM Match : PUCC (HMM E-Value=0.14) 29 4.7 SB_32230| Best HMM Match : Keratin_B2 (HMM E-Value=0.22) 24 7.6 SB_49421| Best HMM Match : 7tm_1 (HMM E-Value=3e-07) 28 8.2 >SB_58242| Best HMM Match : PUCC (HMM E-Value=0.14) Length = 784 Score = 29.1 bits (62), Expect = 4.7 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +3 Query: 555 IQVSIFKFIVRKKGPYFSFPITLCFNRDNSFPVHTTSV 668 IQ S+ ++ GPY+ + R S PVHTTS+ Sbjct: 168 IQHSVRTTSIKHPGPYYRYTAPRSILRVYSTPVHTTSI 205 >SB_32230| Best HMM Match : Keratin_B2 (HMM E-Value=0.22) Length = 254 Score = 24.2 bits (50), Expect(2) = 7.6 Identities = 9/18 (50%), Positives = 11/18 (61%) Frame = +2 Query: 752 YFEYS*NGCPTLTETHYC 805 Y EY PT+ +THYC Sbjct: 130 YCEYPLFRTPTIPDTHYC 147 Score = 22.6 bits (46), Expect(2) = 7.6 Identities = 8/18 (44%), Positives = 12/18 (66%) Frame = +2 Query: 779 PTLTETHYCFXARNYPII 832 PT+ +THYC YP++ Sbjct: 171 PTIADTHYC----KYPLL 184 >SB_49421| Best HMM Match : 7tm_1 (HMM E-Value=3e-07) Length = 265 Score = 28.3 bits (60), Expect = 8.2 Identities = 13/43 (30%), Positives = 23/43 (53%) Frame = -1 Query: 230 IWALNWVTTNVLVILQTSAMIITVDLLNERMAHQQPQTKTDSE 102 IW+ N + + L ++ S II V++ +R+ P T+ D E Sbjct: 153 IWSRNVLAFSSLAVICCSYFIILVNVRKQRIKLSHPSTRRDRE 195 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 24,278,909 Number of Sequences: 59808 Number of extensions: 485394 Number of successful extensions: 819 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 736 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 817 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2383424791 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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