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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P02_F_J17
         (642 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g38840.1 68415.m04772 guanylate-binding family protein simila...    48   4e-06
At5g46070.1 68418.m05665 guanylate-binding family protein contai...    31   0.65 
At2g29580.1 68415.m03592 zinc finger (CCCH-type) family protein ...    30   1.1  
At3g11030.1 68416.m01331 expressed protein contains Pfam domain ...    30   1.5  
At4g11530.1 68417.m01850 protein kinase family protein contains ...    28   6.1  
At1g70060.1 68414.m08061 paired amphipathic helix repeat-contain...    27   8.0  

>At2g38840.1 68415.m04772 guanylate-binding family protein similar
           to SP|Q01514 Interferon-induced guanylate-binding
           protein 1 (Guanine nucleotide-binding protein 1)
           (Interferon-gamma inducible protein MAG-1) {Mus
           musculus}; contains Pfam profile PF02263:
           Guanylate-binding protein, N-terminal domain
          Length = 679

 Score = 48.4 bits (110), Expect = 4e-06
 Identities = 41/123 (33%), Positives = 61/123 (49%), Gaps = 2/123 (1%)
 Frame = +3

Query: 255 VVVISVAGAFRKGKSFLLDFFLRYMHYKYXXXXXXXXXXXSEQDPLQGFSWRGGSERDTT 434
           +  ++V G +R GKSFLL+  L    Y                   +GF      +  T 
Sbjct: 60  ISAVAVIGPYRSGKSFLLNQLLSLSCY-------------------EGFGVGHMRDTKTK 100

Query: 435 GILMWSEIFKATLDDGEKVPIILLDTQGAFDS--ESTVRDCATVFALSTMLSSVQIYNLS 608
           GI +W    +  +D G K  +I LDT+G F+S  +S V D   +FAL+T++SSV IYNL 
Sbjct: 101 GIWVWGTPLELEID-GVKTSVIYLDTEG-FESVGKSNVYD-DRIFALATVMSSVLIYNLP 157

Query: 609 QNI 617
           + +
Sbjct: 158 ETV 160


>At5g46070.1 68418.m05665 guanylate-binding family protein contains
           Pfam domains PF02263: Guanylate-binding protein,
           N-terminal domain and PF02841: Guanylate-binding
           protein, C-terminal domain
          Length = 1060

 Score = 31.1 bits (67), Expect = 0.65
 Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
 Frame = +3

Query: 396 GFSWRGGSERDTTGILMWSEIFKATLDDGEKVPIILLDTQG--AFDSESTVRDCATVFAL 569
           GF      +  T G+ +WS   K T  DG +  ++LLD++G  A+D      D A++  L
Sbjct: 96  GFQVASTHKPCTKGLWLWSSPIKRTALDGTEYNLLLLDSEGIDAYDQTMGGIDEASLDRL 155

Query: 570 S 572
           S
Sbjct: 156 S 156


>At2g29580.1 68415.m03592 zinc finger (CCCH-type) family protein /
           RNA recognition motif (RRM)-containing protein similar
           to SP|O59800 Cell cycle control protein cwf5
           {Schizosaccharomyces pombe}; contains Pfam profile:
           PF00076 RNA recognition motif (aka RRM, RBD, or RNP
           domain)
          Length = 483

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 16/49 (32%), Positives = 19/49 (38%)
 Frame = +2

Query: 80  HKHRERYDHEGEVTEKPAPAPRGPHGVQVVDTGPDHTFTLXRGSPQVSA 226
           H H ++ DH+      P   P  PH   V    PD       GS Q SA
Sbjct: 425 HGHSQQADHDYPQQPGPGSRPNPPHPSSVSAPPPDSVSAAPSGSSQQSA 473


>At3g11030.1 68416.m01331 expressed protein contains Pfam domain
           PF03005: Arabidopsis proteins of unknown function
          Length = 451

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = -3

Query: 148 PTRCRRRLLCHFSLMVIAFPMFMSFVFSH 62
           P+  R+R L HF   V+AF +  +F++ H
Sbjct: 8   PSNRRKRRLTHFFFTVLAFILLAAFIYGH 36


>At4g11530.1 68417.m01850 protein kinase family protein contains
           Pfam PF00069: Protein kinase domain
          Length = 931

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 14/44 (31%), Positives = 20/44 (45%)
 Frame = -3

Query: 304 KKDFPFLKAPATDITTTALSLTSSFRSRDLRASSXQGERMVGAG 173
           KK +   +  ATD  TT  SL  SF++ +          M+G G
Sbjct: 573 KKSYKTTEVQATDEITTTHSLQFSFKTIEAATDKFSDSNMIGRG 616


>At1g70060.1 68414.m08061 paired amphipathic helix repeat-containing
            protein similar to transcription co-repressor Sin3
            [Xenopus laevis] GI:4960210; contains Pfam profile
            PF02671: Paired amphipathic helix repeat
          Length = 1362

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
 Frame = -2

Query: 143  AVPAPASLSLLPHGHSVPDVYVLRFQP-YSIYL 48
            + P+  S+ LL +GH  PDV  +   P ++ YL
Sbjct: 1223 STPSKLSIQLLDYGHDKPDVTSISMDPTFAAYL 1255


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,925,203
Number of Sequences: 28952
Number of extensions: 258331
Number of successful extensions: 917
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 884
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 916
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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