Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_J06
(819 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 260 6e-70
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 252 2e-67
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 246 2e-65
At5g14460.1 68418.m01692 pseudouridylate synthase TruB family pr... 33 0.30
At2g46980.2 68415.m05869 expressed protein 29 2.8
At2g46980.1 68415.m05868 expressed protein 29 2.8
At4g40050.1 68417.m05671 expressed protein 28 6.5
At5g63070.1 68418.m07914 40S ribosomal protein S15, putative 28 8.6
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea... 28 8.6
At4g08470.1 68417.m01398 mitogen-activated protein kinase, putat... 28 8.6
At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138... 28 8.6
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 260 bits (638), Expect = 6e-70
Identities = 121/192 (63%), Positives = 149/192 (77%), Gaps = 1/192 (0%)
Frame = +3
Query: 243 PVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 422
P+V++ GDEMTR+IW +IKEKLILP+LD+++ +DLG+ NRD TDD+VT++ AEA KYN
Sbjct: 81 PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYN 140
Query: 423 VGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPI 602
V IKCATITPDE RV+EF LK MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+KPI
Sbjct: 141 VAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPI 200
Query: 603 IIGRHAHADQYKATDFVVPGAGTLEIIFKPESGEA-IKHVXHXYKGAGVXLAMFNTDASI 779
IGRHA DQY+ATD V+ G G L+++F PE G A ++ + +KG GV LAM+N D SI
Sbjct: 201 CIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALAMYNVDESI 260
Query: 780 IDXAHSSFKFAL 815
A SS AL
Sbjct: 261 RAFAESSMAMAL 272
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 252 bits (617), Expect = 2e-67
Identities = 121/193 (62%), Positives = 145/193 (75%), Gaps = 2/193 (1%)
Frame = +3
Query: 228 KIKA-GPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 404
KIK PVV++ GDEMTR+IW IK+KLI PFL++++ +DLG+ NRD TDD+VTI+ AE
Sbjct: 5 KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64
Query: 405 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 584
A KYNV IKCATITPDE RV EF LKKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV
Sbjct: 65 ATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVP 124
Query: 585 GWDKPIIIGRHAHADQYKATDFVVPGAGTLEIIFKPE-SGEAIKHVXHXYKGAGVXLAMF 761
GW KPI IGRHA DQY+ATD +V G L+++F+P S + + + G GV LAM+
Sbjct: 125 GWTKPICIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALAMY 184
Query: 762 NTDASIIDXAHSS 800
NTD SI A SS
Sbjct: 185 NTDESIRAFAESS 197
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 246 bits (601), Expect = 2e-65
Identities = 117/198 (59%), Positives = 146/198 (73%), Gaps = 1/198 (0%)
Frame = +3
Query: 228 KIK-AGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 404
KIK A P+V++ GDEMTR+IW IK+KLI PF+++++ +DLG+ +RD TDD+VTI+ AE
Sbjct: 5 KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64
Query: 405 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 584
A KKYNV IKCATITPDE RV EF LK+MW+SPNGTIRNIL GTVFRE IICKN+P+LV
Sbjct: 65 ATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVP 124
Query: 585 GWDKPIIIGRHAHADQYKATDFVVPGAGTLEIIFKPESGEAIKHVXHXYKGAGVXLAMFN 764
GW KPI IGRHA DQY+ATD V+ G G L + F+ + G+ V GV +AM+N
Sbjct: 125 GWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMYN 184
Query: 765 TDASIIDXAHSSFKFALD 818
TD SI A +S A +
Sbjct: 185 TDESIRAFADASMNTAYE 202
>At5g14460.1 68418.m01692 pseudouridylate synthase TruB family
protein similar to SP|P09171 tRNA pseudouridine synthase
B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase)
(Pseudouridylate synthase) (Uracil hydrolyase)
{Escherichia coli O157:H7}; contains Pfam profile
PF01509: TruB family pseudouridylate synthase (N
terminal domain)
Length = 540
Score = 32.7 bits (71), Expect = 0.30
Identities = 27/101 (26%), Positives = 43/101 (42%)
Frame = +3
Query: 297 KEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYNVGIKCATITPDEKRVEEF 476
K+K++ V +GMEN D+ DD++ K+ + + EK+VE+F
Sbjct: 222 KKKVVAALGGKRSEVKRIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQF 281
Query: 477 KLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKP 599
K + KSPN + + G E + L WD P
Sbjct: 282 -FKGLTKSPNE--KGMASGGGDGEPFLVTRNGELPPRWDGP 319
>At2g46980.2 68415.m05869 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 2.8
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 629 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 516
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At2g46980.1 68415.m05868 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 2.8
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 629 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 516
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At4g40050.1 68417.m05671 expressed protein
Length = 599
Score = 28.3 bits (60), Expect = 6.5
Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 3/53 (5%)
Frame = -2
Query: 635 VLISMSMTSNDDGFVPPCYKS-RYILANNSLPENSTTKNITD--GPIRAFPHF 486
VL S S T +DD ++P C+ + +L++ S P S+ + + + G ++F HF
Sbjct: 545 VLTSFSRTEDDDPYLPQCHPNLPELLSSVSKPVQSSVQRLAEHLGVAKSF-HF 596
>At5g63070.1 68418.m07914 40S ribosomal protein S15, putative
Length = 160
Score = 27.9 bits (59), Expect = 8.6
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +2
Query: 548 GYYLQEYTETCNRVGQTHHHWTSCSC 625
G+YL E++ TC +V HH C C
Sbjct: 124 GHYLAEFSMTCKKV--NHHRPRICGC 147
>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
family protein similar to katanin p80 subunit
[Strongylocentrotus purpuratus] GI:3005601; contains
Pfam profile PF00400: WD domain, G-beta repeat
Length = 871
Score = 27.9 bits (59), Expect = 8.6
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%)
Frame = +3
Query: 261 GDEMTRIIWDLIKEKLILPF---------LDIELHVYDLGMENRDKTDDQVTIDCAEAIK 413
G++ +WDL KL+ F LD H + L + DKT ++ E I
Sbjct: 255 GEDNVVKVWDLTAGKLLHEFKSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 314
Query: 414 K---YNVGIKCATITPDEKRV 467
G++C T PD K V
Sbjct: 315 SGGTETTGVRCLTFNPDGKSV 335
>At4g08470.1 68417.m01398 mitogen-activated protein kinase, putative
similar to mitogen-activated protein kinase [Arabidopsis
thaliana] gi|1255448|dbj|BAA09057; contains Pfam
PF00069: Protein kinase domain
Length = 560
Score = 27.9 bits (59), Expect = 8.6
Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 4/84 (4%)
Frame = +3
Query: 402 EAIKKYNVGIK--CATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNI-- 569
EAI +VG + C+ +TP + + +K S G + + +N
Sbjct: 237 EAISDSSVGSESTCSLMTPSMEFPDRISFRKRDFSEEGPSGRVKEKRKLMRNKLIENFRK 296
Query: 570 PRLVTGWDKPIIIGRHAHADQYKA 641
P +T W K ++GR ++A Y+A
Sbjct: 297 PEDITSWLKGQLLGRGSYASVYEA 320
>At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 512
Score = 27.9 bits (59), Expect = 8.6
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -3
Query: 544 LKTVPPRILRMVPLGLFH 491
LK +PPR+ R V LGL+H
Sbjct: 176 LKELPPRLKRRVRLGLYH 193
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,130,602
Number of Sequences: 28952
Number of extensions: 366579
Number of successful extensions: 792
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 776
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 790
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1872844800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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