Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_F03
(759 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 50 3e-07
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 44 2e-05
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 38 0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.008
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 32 0.10
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 30 0.41
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 29 0.95
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 1.3
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.7
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 28 1.7
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.2
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 2.2
SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 27 2.9
SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosacchar... 27 3.8
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 27 3.8
SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr 2... 26 5.1
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 6.7
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.7
SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb... 25 8.9
SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog Taz1|Schizosacc... 25 8.9
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 25 8.9
SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 50.4 bits (115), Expect = 3e-07
Identities = 34/123 (27%), Positives = 61/123 (49%), Gaps = 1/123 (0%)
Frame = +1
Query: 358 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSES 537
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 538 -RRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMXRFLANFESDG 714
V +AFK DK G+G IT++++ V + S S EE D+I A+ + DG
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV--LTSLGERLSQEEVADMIRE---ADTDGDG 136
Query: 715 TVD 723
++
Sbjct: 137 VIN 139
Score = 37.1 bits (82), Expect = 0.003
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 346 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 504
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 44.4 bits (100), Expect = 2e-05
Identities = 33/144 (22%), Positives = 67/144 (46%), Gaps = 6/144 (4%)
Frame = +1
Query: 229 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 408
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 409 KETGLELNKSEAEEL-FSQFDTDSSGSISLDEFLIKIRPPMSESRR-----NIVEQAFKK 570
+ NK E + F +D D G IS E + ++ + + R IV++ +
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIME 142
Query: 571 LDKTGDGAITIDDIKGVYSVDSQT 642
+DK DG I+ ++ K + S + T
Sbjct: 143 VDKDRDGKISFEEFKDIVSGSNVT 166
Score = 32.7 bits (71), Expect = 0.058
Identities = 32/135 (23%), Positives = 60/135 (44%), Gaps = 1/135 (0%)
Frame = +1
Query: 346 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRP- 522
F ++D + S +++ EFL I + A LFS D D G + EF+ +
Sbjct: 30 FIKIDANQSGSIDRNEFL-SIPSVA---SNPLASRLFSVVDEDGGGDVDFQEFINSLSVF 85
Query: 523 PMSESRRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMXRFLANF 702
+ ++ ++ AFK D DG I+ ++ V + T + ++ + ++ +
Sbjct: 86 SVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRE-DQLQQIVDKTIM--- 141
Query: 703 ESDGTVDGKVTLEXF 747
E D DGK++ E F
Sbjct: 142 EVDKDRDGKISFEEF 156
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 37.9 bits (84), Expect = 0.002
Identities = 36/129 (27%), Positives = 59/129 (45%), Gaps = 6/129 (4%)
Frame = +1
Query: 379 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVE 555
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 556 QAFKKLDKTGDGAITIDD----IKGVYS-VDSQTRYKSGEETEDVIMXRFLANFESDGTV 720
AF+ D +G I+ D+ + +Y V S + E+T + + + + N D
Sbjct: 103 WAFQLYDLDNNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNK-IFNM-MDKNK 160
Query: 721 DGKVTLEXF 747
DG++TLE F
Sbjct: 161 DGQLTLEEF 169
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.008
Identities = 18/97 (18%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = +1
Query: 334 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL-I 510
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 511 KIRPPMSESRRNIVEQAFKKLDKTGDGAITIDDIKGV 621
+ +++AF+ D G I++ +++ V
Sbjct: 99 MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 33.1 bits (72), Expect = 0.044
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +1
Query: 340 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 507
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 32.7 bits (71), Expect = 0.058
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +1
Query: 424 ELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 603
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 604 DD 609
+D
Sbjct: 93 ED 94
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 31.9 bits (69), Expect = 0.10
Identities = 22/96 (22%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +1
Query: 331 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLI 510
G+ ++FR + S +LN+ EF + GL + E LF + G ++ + F
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYERFTE 546
Query: 511 KIRPPMSESRRNIVEQAFKKLDKTGDGA--ITIDDI 612
+ + + R+ Q DG +T DD+
Sbjct: 547 IVMEELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.9 bits (64), Expect = 0.41
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +1
Query: 391 EFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 570
E+ G+ + + S LF +FD +GS+SL + + I + F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFEL 623
Query: 571 LDKTGDGAITIDDI 612
D GDG + D+
Sbjct: 624 YDFNGDGFMDKPDV 637
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 28.7 bits (61), Expect = 0.95
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +2
Query: 434 KVKPKNSSVNSTQTVVAQSVLMNSLLKSVLLCRNRVVT-L*NKHSRSLTRLVTVQSQL 604
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 28.3 bits (60), Expect = 1.3
Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 2/80 (2%)
Frame = +1
Query: 421 LELNKSEA--EELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGA 594
L LN S EE F + D D SG +S +EF + + ++R IV+ FK+ +G
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEFQHFV--SLLKTRSEIVD-IFKEY-TSGSDK 381
Query: 595 ITIDDIKGVYSVDSQTRYKS 654
++++ + S + R S
Sbjct: 382 MSLEQFRHFLSTSQKARLDS 401
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 27.9 bits (59), Expect = 1.7
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +1
Query: 589 GAITIDDIKGVYSVDSQTRYKSGEETEDV 675
G I+I D GVYS + T YKS E+ E +
Sbjct: 126 GVISIHDSTGVYSQITTTPYKSLEDYEQL 154
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 27.9 bits (59), Expect = 1.7
Identities = 37/150 (24%), Positives = 60/150 (40%), Gaps = 10/150 (6%)
Frame = +1
Query: 274 DPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL 453
D E+ + S +S + L I + GS+L E F I G+ + AE
Sbjct: 793 DNYEQAESIISSLSSSALSELSYISESSMNIGSRL--DERF---IDANGVAIRDFSAELT 847
Query: 454 FSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKK--------LDKTGDGAITIDD 609
+ + +S G +S+D FL K++ + + + F+K DK DD
Sbjct: 848 YLTPE-NSKGKLSIDHFLNKVQSRWHDEEHHYYKTGFRKRVYKYLKIKDKKSKDVDPDDD 906
Query: 610 IKGVYSVDSQT--RYKSGEETEDVIMXRFL 693
+ +++ T RYKS T I R L
Sbjct: 907 LVNQLPLNAYTKPRYKSAASTRLNIYQRIL 936
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 27.5 bits (58), Expect = 2.2
Identities = 22/71 (30%), Positives = 32/71 (45%)
Frame = +1
Query: 394 FLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKL 573
F+ +E+ LE E FSQ +S + SL+ I P SRR IVEQ ++
Sbjct: 680 FILPNEESLLEKYWINYNESFSQLSRESLFT-SLESPFTDIESPTIVSRRKIVEQRKLRM 738
Query: 574 DKTGDGAITID 606
+K +D
Sbjct: 739 EKESFQETNVD 749
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 2.2
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +1
Query: 526 MSESRRNIVEQAFKKLDKTGDGAITIDDIK 615
++ S+ +++AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 488
Score = 27.1 bits (57), Expect = 2.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 166 KPKTSSNIAWHRPMSAGSVQEEELMQK 246
KPK S ++W+ + GS +E EL+ K
Sbjct: 56 KPKKDSLLSWNILLKKGSYKENELLAK 82
>SPAC17G8.05 |med20||mediator complex subunit
Med20|Schizosaccharomyces pombe|chr 1|||Manual
Length = 180
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +1
Query: 628 VDSQTRYKSGEETEDVIMXRFLANFESDGTVDGKV 732
VD T + E ED+I+ L NF T++G +
Sbjct: 55 VDEATMIDAEPELEDIIVRTKLWNFRQSFTIEGSI 89
>SPCC622.12c |||NADP-specific glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 451
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/41 (29%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = +3
Query: 126 GCNVILNSKNVFRKTE--NIFQHCVAQTDVCWFRPGGRADA 242
GC + N+ E +F+ A + CW PG A+A
Sbjct: 331 GCRYVAEGSNMGSSAEAVEVFEKSRASGEGCWLAPGKAANA 371
>SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 413
Score = 26.2 bits (55), Expect = 5.1
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +1
Query: 607 DIKGVYSVDSQTRYKSGEETEDVIMXRFLANFESDG 714
D+ G+ S DS +++ G +DV+ F NFE+ G
Sbjct: 70 DMYGMNS-DSNSQFDGGVNLDDVLAQMFGMNFEAGG 104
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 25.8 bits (54), Expect = 6.7
Identities = 9/34 (26%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -3
Query: 403 HKGIL-LCSVIYFHHHPCAGKFFQVPRSLKHLGT 305
H+G++ +C+++Y KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.7
Identities = 10/34 (29%), Positives = 17/34 (50%)
Frame = -1
Query: 597 DCTVTSLVKLLECLFYNVTTRFRHRRTDFNKEFI 496
D TV + +E LF+N+T +H F + +
Sbjct: 699 DATVANRKDFIEFLFHNITVSSKHTAVIFTSDLL 732
>SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 840
Score = 25.4 bits (53), Expect = 8.9
Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 5/60 (8%)
Frame = +1
Query: 439 EAEELFSQFDTDSSGSISLDEF-----LIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 603
E ++F D D S +++LDE I I S V+ A KLD+ G G + I
Sbjct: 396 EINDIFHILDNDYSRTVTLDEMEQFTREISIEFRSISSSLRDVDLALGKLDRVGLGVVGI 455
>SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog
Taz1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 663
Score = 25.4 bits (53), Expect = 8.9
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +1
Query: 445 EELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIV 552
E + F TDS+ SI EF++ P S N+V
Sbjct: 273 ELVIRYFGTDSNPSIDASEFILSCLPYTSLDALNVV 308
>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 230
Score = 25.4 bits (53), Expect = 8.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 152 KCI*ENRKHLPTLRGTDRCLLVPSRRK 232
+C+ E+R+H P L +D LL +RK
Sbjct: 82 RCMEEDREHNPFLSSSDNQLLSRKKRK 108
>SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 497
Score = 25.4 bits (53), Expect = 8.9
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +1
Query: 217 SVQEEELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEF 396
S Q E + R M L +L + ++ ILGL R D+G K+ NK+
Sbjct: 182 SYQNLESINSWLRLMASLYKDLSELVPVGITAEGRTILGLKLNGRHPSDNGEKIRNKKVI 241
Query: 397 L-----YGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 504
+ + + G+ A +L +++D+D LD+F
Sbjct: 242 IIQGGSHAREWIGIPSVCYAAWQLLAKYDSDGHVRKLLDKF 282
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,880,451
Number of Sequences: 5004
Number of extensions: 57695
Number of successful extensions: 199
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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