Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_D15
(878 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53480.1 68418.m06646 importin beta-2, putative similar to im... 173 1e-43
At4g27640.1 68417.m03973 importin beta-2 subunit family protein ... 56 4e-08
At2g16950.1 68415.m01953 importin beta-2 subunit family protein ... 50 2e-06
At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -relat... 31 1.0
At5g13590.1 68418.m01572 expressed protein 29 3.1
At3g25840.1 68416.m03219 protein kinase family protein contains ... 29 3.1
At5g10140.1 68418.m01174 MADS-box protein flowering locus F (FLF... 29 5.4
At1g11050.1 68414.m01266 protein kinase family protein contains ... 29 5.4
At5g53700.1 68418.m06672 hypothetical protein 28 9.5
At5g49830.1 68418.m06171 expressed protein 28 9.5
At3g27510.1 68416.m03439 DC1 domain-containing protein contains ... 28 9.5
At2g34280.1 68415.m04194 S locus F-box-related / SLF-related con... 28 9.5
At1g73460.1 68414.m08504 protein kinase family protein contains ... 28 9.5
>At5g53480.1 68418.m06646 importin beta-2, putative similar to
importin-beta2 [Oryza sativa (japonica cultivar-group)]
GI:3983665; contains Pfam profile PF03810: Importin-beta
N-terminal domain
Length = 870
Score = 173 bits (421), Expect = 1e-43
Identities = 105/279 (37%), Positives = 158/279 (56%), Gaps = 2/279 (0%)
Frame = +2
Query: 47 LTLIQILEKTVSPDRNELEAAVRYLDHAATTNFTTFIKMLSDVLLQGGNSQVARMAAGLQ 226
+ + Q+L S D + A L N F+ L+ L +R AGL
Sbjct: 3 MEVTQLLINAQSIDGTVRKHAEESLKQFQEQNLAGFLLSLAGELANDEKPVDSRKLAGLV 62
Query: 227 LKNHLTSKDPTLKQQYQQRWLALAEDVRLYIKENILAAIGTE-NSRPSSAAQCVAYVAVA 403
LKN L +K+ K + QRWLAL + I+ +L + S+A+Q +A VA
Sbjct: 63 LKNALDAKEQHRKYELVQRWLALDMSTKSQIRAFLLKTLSAPVPDVRSTASQVIAKVAGI 122
Query: 404 ELPVGQWNDLIPILVENVVHVQSTELKKEATLEAIGYICQDIDAEVLT-ERSNQILTAII 580
ELP QW +LI L+ N+ H +K +ATLE +GY+C+++ +V+ E N+ILTA++
Sbjct: 123 ELPQKQWPELIVSLLSNI-HQLPAHVK-QATLETLGYLCEEVSPDVVEQEHVNKILTAVV 180
Query: 581 HGMRSTEPSNHVRLAATQALLNSLEFTRANFEKENERNFIMEVVCEATQSSDMRISVAAL 760
GM + E + VRLAAT+AL +L F +ANF + ER++IM VVCEAT S +++I AA
Sbjct: 181 QGMNAAEGNTDVRLAATRALYMALGFAQANFNNDMERDYIMRVVCEATLSPEVKIRQAAF 240
Query: 761 QCLVKILSLYYQHMEPYMGQALFPITLEAMKSDIDXISL 877
+CLV I S YY+ + YM Q +F IT +A++ D + ++L
Sbjct: 241 ECLVSIASTYYEKLAHYM-QDIFNITAKAVREDDESVAL 278
>At4g27640.1 68417.m03973 importin beta-2 subunit family protein low
similarity to importin 4 GI:18700635 from [Homo sapiens]
Length = 1048
Score = 55.6 bits (128), Expect = 4e-08
Identities = 41/153 (26%), Positives = 76/153 (49%), Gaps = 2/153 (1%)
Frame = +2
Query: 260 LKQQYQQRWLALAEDVRLYIKENILAAIGTENSRP--SSAAQCVAYVAVAELPVGQWNDL 433
L+++ W L+ ++ ++K++++ +I ENS P ++A V+ VA +P G+W DL
Sbjct: 60 LRKRITGHWAKLSPQLKQHVKQSLIESITVENSPPVRRASANVVSVVAKYAVPAGEWPDL 119
Query: 434 IPILVENVVHVQSTELKKEATLEAIGYICQDIDAEVLTERSNQILTAIIHGMRSTEPSNH 613
+ L + Q E +E L + + I ++ L A++ E S+
Sbjct: 120 LTFLFQCSQSAQ--EDHREVALILFSSLTETIGNTFRPYFAD--LQALLLKCMQDESSSR 175
Query: 614 VRLAATQALLNSLEFTRANFEKENERNFIMEVV 712
VR+AA +A+ + LEFT E R+FI ++
Sbjct: 176 VRVAALKAVGSFLEFTNDGDEVVKFRDFIPSIL 208
Score = 34.3 bits (75), Expect = 0.11
Identities = 23/70 (32%), Positives = 42/70 (60%), Gaps = 1/70 (1%)
Frame = +2
Query: 434 IPILVENVVHVQSTELK-KEATLEAIGYICQDIDAEVLTERSNQILTAIIHGMRSTEPSN 610
+P+L VH QST LK +EA++ A+G I + +++ E+ + +L ++ +R +P
Sbjct: 336 LPVLEFASVHCQSTNLKFREASVTALGVISEGC-FDLMKEKLDTVLNIVLGALR--DPEL 392
Query: 611 HVRLAATQAL 640
VR AA+ A+
Sbjct: 393 VVRGAASFAI 402
>At2g16950.1 68415.m01953 importin beta-2 subunit family protein
similar to SP|Q92973 Importin beta-2 subunit
(Transportin) {Homo sapiens}; contains Pfam profile
PF03810: Importin-beta N-terminal domain
Length = 891
Score = 50.4 bits (115), Expect = 2e-06
Identities = 46/169 (27%), Positives = 79/169 (46%), Gaps = 3/169 (1%)
Frame = +2
Query: 53 LIQILEKTVSPDRNELEAAV-RYLDHAATTNFTTFIKMLSDVLLQG-GNSQVARMAAGLQ 226
+ +LE+ +SP ++ + + L H + F F L +L++ G S R AAGL
Sbjct: 18 ICSLLEQQISPSSVVDKSQIWKQLQHFS--QFPDFNNYLVFILVRAEGKSVEVRQAAGLL 75
Query: 227 LKNHLTSKDPTLKQQYQQRWLALAEDVRLYIKENILAAIGTENSRPSSAAQCVAYVAVAE 406
LKN+L P++ Q+ Q+ YIK +L +G + + + V V
Sbjct: 76 LKNNLRGAYPSMTQENQK-----------YIKSELLPCLGAADRNIRTTVGTIISVIVNI 124
Query: 407 LPVGQWNDLIPILVENVVHVQSTELK-KEATLEAIGYICQDIDAEVLTE 550
V W++L+P L V + S +L + ++A+ IC+DI + TE
Sbjct: 125 EGVSGWHELLPAL---VTCLDSNDLNHMDGAMDALSKICEDIPHVLDTE 170
>At2g20190.1 68415.m02361 CLIP-associating protein (CLASP) -related
similar to CLIP-associating protein CLASP2 (GI:13508651)
[Rattus norvegicus]
Length = 1439
Score = 31.1 bits (67), Expect = 1.0
Identities = 21/78 (26%), Positives = 39/78 (50%)
Frame = +2
Query: 617 RLAATQALLNSLEFTRANFEKENERNFIMEVVCEATQSSDMRISVAALQCLVKILSLYYQ 796
R+AA + L LE +R + E +++ + + S+ R+S ALQ L L +
Sbjct: 16 RMAAVERLHQLLEASRKSLSPA-EVTSLVDSCLDLLKDSNFRVSQGALQALASAAVLAGE 74
Query: 797 HMEPYMGQALFPITLEAM 850
H++ ++ AL P +E +
Sbjct: 75 HLKLHL-NALVPAVVERL 91
>At5g13590.1 68418.m01572 expressed protein
Length = 1190
Score = 29.5 bits (63), Expect = 3.1
Identities = 19/69 (27%), Positives = 30/69 (43%)
Frame = +2
Query: 602 PSNHVRLAATQALLNSLEFTRANFEKENERNFIMEVVCEATQSSDMRISVAALQCLVKIL 781
PSN R+ + +L + + R +KE R C+ SS + L C K
Sbjct: 111 PSNQTRMKVEKTVLKTHDIVRKTGDKETLRG-----ECQTEASSGAKTVSLQLSCNTKNN 165
Query: 782 SLYYQHMEP 808
S Y+++ EP
Sbjct: 166 SPYWKNEEP 174
>At3g25840.1 68416.m03219 protein kinase family protein contains
Pfam profile: PF00069 eukaryotic protein kinase domain
Length = 935
Score = 29.5 bits (63), Expect = 3.1
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 11 MKNSETMHAETTLTLIQILEKTVSPDRNELEAAVRYL 121
++N+ETMH + +QIL+K DR + VR+L
Sbjct: 652 IRNNETMHKAGKIE-VQILKKLAGADREDRRHCVRFL 687
>At5g10140.1 68418.m01174 MADS-box protein flowering locus F (FLF)
identical to FLOWERING LOCUS C protein (MADS box protein
FLOWERING LOCUS F) (Swiss-Prot:Q9S7Q7) [Arabidopsis
thaliana]
Length = 196
Score = 28.7 bits (61), Expect = 5.4
Identities = 37/137 (27%), Positives = 68/137 (49%), Gaps = 7/137 (5%)
Frame = +2
Query: 53 LIQILEKTVSPDRNELEAAVRYLDHAATT-NFTTFIKMLS--DVLLQGGNSQVARMAAGL 223
L++IL++ ++L+A LDH + N+ + ++L D L G N + + A +
Sbjct: 63 LVKILDRYGKQHADDLKA----LDHQSKALNYGSHYELLELVDSKLVGSNVKNVSIDALV 118
Query: 224 QLKNHLTSKDPTLKQQYQQRWLALAEDVRLYIKENILAAIGTENSRPSSAAQCVAYV-AV 400
QL+ HL + + + + L L E+++ KE +L EN +S + +V A
Sbjct: 119 QLEEHLETALSVTRAKKTELMLKLVENLK--EKEKMLK---EENQVLASQMENNHHVGAE 173
Query: 401 AEL---PVGQWNDLIPI 442
AE+ P GQ +D +P+
Sbjct: 174 AEMEMSPAGQISDNLPV 190
>At1g11050.1 68414.m01266 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 625
Score = 28.7 bits (61), Expect = 5.4
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 141 ISQHSSKCCPMSFSKVVTVKW 203
++Q S+ CP+ FS V+T+ W
Sbjct: 19 VAQSPSQTCPLDFSHVLTIPW 39
>At5g53700.1 68418.m06672 hypothetical protein
Length = 248
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = -2
Query: 250 FRSEMIFQLQTSCHPSHLTVTTLEKDIGQHFDECCEICGGSMVEVSNCC 104
F+ E ++ PS L+ L+ + +HF C E+ G S++ N C
Sbjct: 115 FKGERTLIVKVYDTPSTLSKIDLQIGLCKHFSSCGEVTGISVLVHGNLC 163
>At5g49830.1 68418.m06171 expressed protein
Length = 752
Score = 27.9 bits (59), Expect = 9.5
Identities = 20/82 (24%), Positives = 43/82 (52%), Gaps = 1/82 (1%)
Frame = +2
Query: 527 IDAEVLTERSNQILTAIIHGMRSTEPSNHVRLAATQALLNSLEFTRANFEKENERNFIME 706
+DA + R ++ L A G +N + + ++L+SL+F A K+ + + +
Sbjct: 151 LDALLAERRVDEALAAFDEGEILVSQANE-KHTLSSSVLSSLQFAIAE-RKQKLADQLAK 208
Query: 707 VVCE-ATQSSDMRISVAALQCL 769
C+ +T+ ++R ++AAL+ L
Sbjct: 209 AACQPSTRGGELRSAIAALKRL 230
>At3g27510.1 68416.m03439 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 566
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 5/50 (10%)
Frame = -2
Query: 262 KCRIFRSE---MIFQLQTSCHPSHLT--VTTLEKDIGQHFDECCEICGGS 128
+CR+ E +F+ HPSH+ + + + D C+ICGG+
Sbjct: 152 RCRLVIHEGCVSVFESPEIIHPSHVRHPLKLISSGAPDYTDRSCDICGGT 201
>At2g34280.1 68415.m04194 S locus F-box-related / SLF-related
contains Pfam PF00646: F-box domain; contains weak hit
to TIGRFAM TIGR01640 : F-box protein interaction domain;
weakly similar to self-incompatibility (S-) locus F-box
(GI:29420811) [Prunus mume]
Length = 391
Score = 27.9 bits (59), Expect = 9.5
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = -2
Query: 316 IKSNIFCQSQPSLLILLFKCRIFRSEMIFQLQTSCHPSHLTVTTLEK 176
+KS + C+S +CR F+ + + SC+P L V+ ++
Sbjct: 18 VKSLLNCKSVSKQWRSTIRCRAFQERQLMHRRQSCNPDVLLVSVADE 64
>At1g73460.1 68414.m08504 protein kinase family protein contains
protein kinase domain Pfam:PF00069
Length = 1169
Score = 27.9 bits (59), Expect = 9.5
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = +2
Query: 2 GRPMKNSETMHAETTLTLIQILEKTVSPDRN--ELEAAVRYLDHAAT 136
G K+ ++MHAE++ +L +TV+ DRN L A+ D AT
Sbjct: 692 GSSQKDGQSMHAESSKSLWSGNHETVTRDRNTERLSASTAMDDMVAT 738
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,319,556
Number of Sequences: 28952
Number of extensions: 330098
Number of successful extensions: 887
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 882
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2067932800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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