Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_D14
(826 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g12780.1 68416.m01596 phosphoglycerate kinase, putative simil... 185 3e-47
At1g56190.1 68414.m06458 phosphoglycerate kinase, putative simil... 181 4e-46
At1g79550.2 68414.m09274 phosphoglycerate kinase, putative simil... 176 1e-44
At1g79550.1 68414.m09273 phosphoglycerate kinase, putative simil... 176 1e-44
At3g14910.1 68416.m01885 expressed protein 33 0.30
At1g34590.1 68414.m04299 hypothetical protein 32 0.53
At5g49250.1 68418.m06096 hypothetical protein 29 2.8
At2g33200.1 68415.m04067 F-box family protein contains F-box dom... 29 2.8
At2g47250.1 68415.m05900 RNA helicase, putative similar to SP|P5... 29 5.0
At1g21590.1 68414.m02699 protein kinase family protein contains ... 29 5.0
At3g03850.1 68416.m00396 auxin-responsive protein, putative simi... 28 8.7
At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger) fa... 28 8.7
>At3g12780.1 68416.m01596 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 481
Score = 185 bits (451), Expect = 3e-47
Identities = 104/234 (44%), Positives = 147/234 (62%), Gaps = 3/234 (1%)
Frame = +3
Query: 132 IDALNLTGKRVLMRVDFNVPLKEG-VITNNQRIVAALDSVKYALDKGAKSVVLMSHLGRP 308
+ + +L GK+V +R D NVPL + IT++ RI AA+ ++KY ++ GAK V+L +HLGRP
Sbjct: 85 LTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAK-VILSTHLGRP 143
Query: 309 DGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHIEE 488
G V K++L P+ L +LL +VT +DCIGPEVE+ A+ G ++LLEN+RF+ EE
Sbjct: 144 KG-VTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFYKEE 202
Query: 489 EGXGVDASGAKVKADPEKVKAFRASLRKLGDVYINDAFGTAHRAHSSMVG--EGFEQRAS 662
E K DPE F L L D+Y+NDAFGTAHRAH+S G + + +
Sbjct: 203 E-----------KNDPE----FAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVA 247
Query: 663 GFLLKKELQYFAKALHEPERPFLAILGGAKVXXKXXXXXXXXDKVNEMIIGGGM 824
GFLL+KEL Y A+ P+RPF AI+GG+KV K +K + +++GGGM
Sbjct: 248 GFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM 301
>At1g56190.1 68414.m06458 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 478
Score = 181 bits (441), Expect = 4e-46
Identities = 101/234 (43%), Positives = 148/234 (63%), Gaps = 3/234 (1%)
Frame = +3
Query: 132 IDALNLTGKRVLMRVDFNVPLKEGV-ITNNQRIVAALDSVKYALDKGAKSVVLMSHLGRP 308
+++++L GK+V +R D NVPL + IT++ RI AA+ ++K+ ++ GAK V+L +HLGRP
Sbjct: 82 LNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAK-VILSTHLGRP 140
Query: 309 DGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHIEE 488
G V K++L P+ L +LL +V +DCIGPEVET A+ G ++LLEN+RF+ EE
Sbjct: 141 KG-VTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFYKEE 199
Query: 489 EGXGVDASGAKVKADPEKVKAFRASLRKLGDVYINDAFGTAHRAHSSMVG--EGFEQRAS 662
E K +P+ F L L D+Y+NDAFGTAHRAH+S G + + +
Sbjct: 200 E-----------KNEPD----FAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVA 244
Query: 663 GFLLKKELQYFAKALHEPERPFLAILGGAKVXXKXXXXXXXXDKVNEMIIGGGM 824
GFLL+KEL Y A+ P+RPF AI+GG+KV K +K + +++GGGM
Sbjct: 245 GFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGM 298
>At1g79550.2 68414.m09274 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 401
Score = 176 bits (429), Expect = 1e-44
Identities = 103/230 (44%), Positives = 140/230 (60%), Gaps = 3/230 (1%)
Frame = +3
Query: 144 NLTGKRVLMRVDFNVPLKEGV-ITNNQRIVAALDSVKYALDKGAKSVVLMSHLGRPDGQV 320
+L GK V +RVD NVPL + IT++ RI AA+ ++KY + G++ VVL SHLGRP G V
Sbjct: 14 DLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSR-VVLCSHLGRPKG-V 71
Query: 321 NLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHIEEEGXG 500
KY+LKP+ L +LL +V ND IG EV+ A G ++LLEN+RF+ EEE
Sbjct: 72 TPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFYAEEE--- 128
Query: 501 VDASGAKVKADPEKVKAFRASLRKLGDVYINDAFGTAHRAHSSM--VGEGFEQRASGFLL 674
K DPE F L L DVY+NDAFGTAHRAH+S V + + +GFL+
Sbjct: 129 --------KNDPE----FAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLM 176
Query: 675 KKELQYFAKALHEPERPFLAILGGAKVXXKXXXXXXXXDKVNEMIIGGGM 824
+KEL Y A+ P++PF AI+GG+KV K + V+ +++GGGM
Sbjct: 177 QKELDYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGM 226
>At1g79550.1 68414.m09273 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 401
Score = 176 bits (429), Expect = 1e-44
Identities = 103/230 (44%), Positives = 140/230 (60%), Gaps = 3/230 (1%)
Frame = +3
Query: 144 NLTGKRVLMRVDFNVPLKEGV-ITNNQRIVAALDSVKYALDKGAKSVVLMSHLGRPDGQV 320
+L GK V +RVD NVPL + IT++ RI AA+ ++KY + G++ VVL SHLGRP G V
Sbjct: 14 DLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSR-VVLCSHLGRPKG-V 71
Query: 321 NLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHIEEEGXG 500
KY+LKP+ L +LL +V ND IG EV+ A G ++LLEN+RF+ EEE
Sbjct: 72 TPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFYAEEE--- 128
Query: 501 VDASGAKVKADPEKVKAFRASLRKLGDVYINDAFGTAHRAHSSM--VGEGFEQRASGFLL 674
K DPE F L L DVY+NDAFGTAHRAH+S V + + +GFL+
Sbjct: 129 --------KNDPE----FAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLM 176
Query: 675 KKELQYFAKALHEPERPFLAILGGAKVXXKXXXXXXXXDKVNEMIIGGGM 824
+KEL Y A+ P++PF AI+GG+KV K + V+ +++GGGM
Sbjct: 177 QKELDYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGM 226
>At3g14910.1 68416.m01885 expressed protein
Length = 455
Score = 32.7 bits (71), Expect = 0.30
Identities = 19/55 (34%), Positives = 32/55 (58%), Gaps = 1/55 (1%)
Frame = +3
Query: 195 KEGVITNNQRIVAALDSVKYALDKGAKSVVLMSHLGRPDG-QVNLKYTLKPVAEE 356
KEG + NN +++ALDS AL + ++L+ + G PD +V ++ L P+ E
Sbjct: 25 KEGWLVNNPNLLSALDSHSLAL--ANRFLILIVNWGDPDAPRVKIRPDLSPIEAE 77
>At1g34590.1 68414.m04299 hypothetical protein
Length = 820
Score = 31.9 bits (69), Expect = 0.53
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = -1
Query: 478 WKRRFSSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATGLRVYLR 323
W + FS +E A L H VS ++SF + T F + S+ T VY +
Sbjct: 349 WLKHFSRERVERALRLLHGVSCPTSLESFDHRTQFFVDMQSTKLTLWEVYAK 400
>At5g49250.1 68418.m06096 hypothetical protein
Length = 200
Score = 29.5 bits (63), Expect = 2.8
Identities = 19/66 (28%), Positives = 33/66 (50%)
Frame = +1
Query: 172 VWISMYRLKKE*LQTTSAL*LP*IPLNMLWIKALNQLYLCRIWVDRMAR*ISSTPSNQSL 351
VW+ +Y ++ L T AL P L ++ + +LN+ Y C I + ++ P + SL
Sbjct: 29 VWVFIYLMRTLVLATFKALWYPYKELKIVVLSSLNRSYCCGICGIGLFFFVAHLPLSSSL 88
Query: 352 KS*KNC 369
K+C
Sbjct: 89 FVFKHC 94
>At2g33200.1 68415.m04067 F-box family protein contains F-box domain
Pfam:PF00646
Length = 376
Score = 29.5 bits (63), Expect = 2.8
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 3/54 (5%)
Frame = -1
Query: 478 WKRRF---SSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATGLRVYL 326
W+ +F S+ + +P E H + G S +NV + SN+F +GL YL
Sbjct: 52 WRIKFNKISTSLFDPREDKIHEIQHPGIEFSDRNVLASCSNWFLMVDSGLEFYL 105
>At2g47250.1 68415.m05900 RNA helicase, putative similar to
SP|P53131 Pre-mRNA splicing factor RNA helicase PRP43
(Helicase JA1) {Saccharomyces cerevisiae}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 729
Score = 28.7 bits (61), Expect = 5.0
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = +3
Query: 264 KGAKSVVLMSHLGRPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGP 410
+ A V+ H+ P G + + T + E+ + +NK+V+ L D +GP
Sbjct: 264 EAAIRTVVQIHMCEPPGDILVFLTGEEEIEDACRKINKEVSNLGDQVGP 312
>At1g21590.1 68414.m02699 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 756
Score = 28.7 bits (61), Expect = 5.0
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -1
Query: 463 SSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATG 341
S R E EGL +STS ++K + S+ SNF + + G
Sbjct: 376 SGRFPENVEGLQARISTSCQFFTYKELVSVTSNFCADNFIG 416
>At3g03850.1 68416.m00396 auxin-responsive protein, putative similar
to small auxin-up regulated protein SAUR (GI:3043536)
[Raphanus sativus]
Length = 93
Score = 27.9 bits (59), Expect = 8.7
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -3
Query: 623 VRSMCSTKSIINIDITKFSQACPKGF 546
VRS+ S K I+ + K S+A PKGF
Sbjct: 4 VRSLFSAKKILGGSLVKTSKAPPKGF 29
>At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 383
Score = 27.9 bits (59), Expect = 8.7
Identities = 20/80 (25%), Positives = 34/80 (42%)
Frame = +3
Query: 348 AEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIILLENLRFHIEEEGXGVDASGAKVK 527
AEELK +L++ L E E+AC A + + LR ++ V +K
Sbjct: 70 AEELKNVLDEHFLELRVKAAHETESACQERLATAKAEIAELRTQLDLSEREVLELKEGIK 129
Query: 528 ADPEKVKAFRASLRKLGDVY 587
++ +A A + +G Y
Sbjct: 130 VKEQEAEASIAEMETIGQAY 149
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,914,758
Number of Sequences: 28952
Number of extensions: 302326
Number of successful extensions: 771
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 742
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 756
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1892353600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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