Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_D11
(833 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) / putre... 164 7e-41
At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) / putre... 162 3e-40
At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) / putre... 162 3e-40
At5g53120.3 68418.m06603 spermidine synthase, putative / putresc... 159 2e-39
At5g53120.2 68418.m06602 spermidine synthase, putative / putresc... 159 2e-39
At5g53120.1 68418.m06601 spermidine synthase, putative / putresc... 159 2e-39
At5g19530.1 68418.m02326 spermine/spermidine synthase family pro... 107 9e-24
At2g04920.1 68415.m00513 F-box family protein (FBX9) identical t... 31 0.94
At3g44190.1 68416.m04738 pyridine nucleotide-disulphide oxidored... 31 1.2
At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4... 30 1.6
At3g16470.1 68416.m02101 jacalin lectin family protein contains ... 29 2.9
At2g29720.1 68415.m03612 monooxygenase family protein nearly ide... 29 2.9
At5g22140.2 68418.m02577 pyridine nucleotide-disulphide oxidored... 29 3.8
At5g22140.1 68418.m02578 pyridine nucleotide-disulphide oxidored... 29 3.8
At4g13330.1 68417.m02083 expressed protein 29 3.8
At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family... 29 3.8
At3g04350.1 68416.m00460 expressed protein 29 3.8
At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyc... 28 6.6
At1g14460.1 68414.m01715 DNA polymerase-related weak similarity ... 28 8.8
>At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) /
putrescine aminopropyltransferase 2 identical to
SP|O48661 Spermidine synthase 2 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 2) (SPDSY 2)
{Arabidopsis thaliana}
Length = 340
Score = 164 bits (398), Expect = 7e-41
Identities = 72/144 (50%), Positives = 103/144 (71%)
Frame = +2
Query: 401 MDKLKTNWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCT 580
M + WF+E MWPG S +V+++L KS YQ++ VF + + GKVLVLDG+IQ T
Sbjct: 44 MSSIIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLT 103
Query: 581 QKDEFSYQEMISFLPLCCHKNPENVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIEL 760
++DE +YQEMI+ LPLC NP+ VL++GGGDGGV REVA+H V+ I + EID V+++
Sbjct: 104 ERDECAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDV 163
Query: 761 SKRYLPFMAVGSDSPKLTLHVGDG 832
+K+Y P +AVG + P++ L +GDG
Sbjct: 164 AKQYFPNVAVGYEDPRVNLIIGDG 187
>At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 283
Score = 162 bits (393), Expect = 3e-40
Identities = 70/137 (51%), Positives = 101/137 (73%)
Frame = +2
Query: 422 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 601
WF+E MWPG S +V++VL KS YQ++ VF + + GKVLVLDG+IQ T++DE +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 602 QEMISFLPLCCHKNPENVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPF 781
QEMI+ LPLC NP+ VL++GGGDGGV REVA+H ++ I + EID V+++SK++ P
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPD 166
Query: 782 MAVGSDSPKLTLHVGDG 832
+A+G + P++ L +GDG
Sbjct: 167 VAIGYEDPRVNLVIGDG 183
>At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 334
Score = 162 bits (393), Expect = 3e-40
Identities = 70/137 (51%), Positives = 101/137 (73%)
Frame = +2
Query: 422 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 601
WF+E MWPG S +V++VL KS YQ++ VF + + GKVLVLDG+IQ T++DE +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 602 QEMISFLPLCCHKNPENVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPF 781
QEMI+ LPLC NP+ VL++GGGDGGV REVA+H ++ I + EID V+++SK++ P
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPD 166
Query: 782 MAVGSDSPKLTLHVGDG 832
+A+G + P++ L +GDG
Sbjct: 167 VAIGYEDPRVNLVIGDG 183
>At5g53120.3 68418.m06603 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 159 bits (387), Expect = 2e-39
Identities = 70/129 (54%), Positives = 99/129 (76%)
Frame = +2
Query: 443 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 622
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 623 PLCCHKNPENVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPFMAVGSDS 802
PLC +P+NVL+VGGGDGGV RE+++H V+ I + EID VI++SK++ P +AVG D
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDD 196
Query: 803 PKLTLHVGD 829
P++ LH+GD
Sbjct: 197 PRVQLHIGD 205
>At5g53120.2 68418.m06602 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 159 bits (387), Expect = 2e-39
Identities = 70/129 (54%), Positives = 99/129 (76%)
Frame = +2
Query: 443 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 622
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 623 PLCCHKNPENVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPFMAVGSDS 802
PLC +P+NVL+VGGGDGGV RE+++H V+ I + EID VI++SK++ P +AVG D
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDD 196
Query: 803 PKLTLHVGD 829
P++ LH+GD
Sbjct: 197 PRVQLHIGD 205
>At5g53120.1 68418.m06601 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 159 bits (387), Expect = 2e-39
Identities = 70/129 (54%), Positives = 99/129 (76%)
Frame = +2
Query: 443 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 622
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 623 PLCCHKNPENVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPFMAVGSDS 802
PLC +P+NVL+VGGGDGGV RE+++H V+ I + EID VI++SK++ P +AVG D
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDD 196
Query: 803 PKLTLHVGD 829
P++ LH+GD
Sbjct: 197 PRVQLHIGD 205
>At5g19530.1 68418.m02326 spermine/spermidine synthase family
protein similar to SP|P09158 Spermidine synthase (EC
2.5.1.16) (Putrescine aminopropyltransferase)
{Escherichia coli}; contains Pfam profile PF01564:
Spermine/spermidine synthase
Length = 339
Score = 107 bits (257), Expect = 9e-24
Identities = 52/137 (37%), Positives = 81/137 (59%)
Frame = +2
Query: 419 NWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFS 598
+W+ E+ D +SF + VLH S+YQ+I + DT GKVLV+DG +Q ++DEF
Sbjct: 34 HWYEETID--DDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFI 91
Query: 599 YQEMISFLPLCCHKNPENVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRYLP 778
Y E + L H NP+ V I+GGG+G ARE+ KH ++ +V+ +ID V++ +R+L
Sbjct: 92 YHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFLT 151
Query: 779 FMAVGSDSPKLTLHVGD 829
+ + KL L + D
Sbjct: 152 VNSDAFCNKKLELVIKD 168
>At2g04920.1 68415.m00513 F-box family protein (FBX9) identical to
F-box protein family, AtFBX9 (GI:20197985) [Arabidopsis
thaliana]; contains F-box domain PF:00646; contains
TIGRFAM TIGR01640 : F-box protein interaction domain
Length = 376
Score = 31.1 bits (67), Expect = 0.94
Identities = 17/42 (40%), Positives = 23/42 (54%)
Frame = +2
Query: 464 SFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKD 589
S E K VL + S Y + QV+ + KV DG++ CT KD
Sbjct: 75 SIEFKSVLSLKDSHYNSEQVY----IAKVFHCDGLLLCTTKD 112
>At3g44190.1 68416.m04738 pyridine nucleotide-disulphide
oxidoreductase family protein low similarity to
dihydrolipoamide dehydrogenase from Clostridium magnum
[GI:472330]; contains Pfam profile PF00070 Pyridine
nucleotide-disulphide oxidoreductase domain
Length = 367
Score = 30.7 bits (66), Expect = 1.2
Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 2/42 (4%)
Frame = +2
Query: 638 KNPENVLIVGGGDGGV--AREVAKHPQVKHIVLVEIDDRVIE 757
K+ E++LIVGGG GV A E+A K + +V R++E
Sbjct: 132 KSSESILIVGGGPSGVELAAEIAVDFPEKKVTIVHNGPRLLE 173
>At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4)
identical to CBL-interacting protein kinase 4
[Arabidopsis thaliana] gi|13249503|gb|AAG01367;
identical to cDNA calcineurin B-like (CBL) interacting
protein kinase 4 (CIPK4) GI:13249502
Length = 426
Score = 30.3 bits (65), Expect = 1.6
Identities = 18/67 (26%), Positives = 29/67 (43%)
Frame = +2
Query: 416 TNWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEF 595
T WF +S ++ + FE+ L E I FD SL L L G+ + ++ E
Sbjct: 274 TVWFQKSLEISEFQSSVFELDRFLEKEAKSSNAITAFDLISLSSGLDLSGLFERRKRKEK 333
Query: 596 SYQEMIS 616
+ +S
Sbjct: 334 RFTARVS 340
>At3g16470.1 68416.m02101 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase-binding protein homolog
[Arabidopsis thaliana] GI:2997767
Length = 451
Score = 29.5 bits (63), Expect = 2.9
Identities = 21/58 (36%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = +2
Query: 650 NVLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRY--LPFMAVGSDSPKLTL 817
N + GGDGGVA + H VK I + + D V Y +GSD K TL
Sbjct: 307 NTIPAQGGDGGVAWDDGVHDSVKKIYVGQGDSCVTYFKADYEKASKPVLGSDHGKKTL 364
>At2g29720.1 68415.m03612 monooxygenase family protein nearly
identical to CTF2B [GI:4164578][Plant Physiol. 119, 364
(1999), PGR99-008]
Length = 427
Score = 29.5 bits (63), Expect = 2.9
Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +2
Query: 647 ENVLIVGGGDGGVAREVAKHP-QVKHIVLVEID 742
E V+IVGGG GG+A VA H ++ +VL + +
Sbjct: 43 EKVVIVGGGIGGLATAVALHRLGIRSVVLEQAE 75
>At5g22140.2 68418.m02577 pyridine nucleotide-disulphide
oxidoreductase family protein contains Pfam profile
PF00070 Pyridine nucleotide-disulphide oxidoreductase
domain
Length = 311
Score = 29.1 bits (62), Expect = 3.8
Identities = 18/42 (42%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +2
Query: 638 KNPENVLIVGGGDGGV--AREVAKHPQVKHIVLVEIDDRVIE 757
K+ +VLIVGGG GV A E+A K + LV R++E
Sbjct: 78 KSSGSVLIVGGGPSGVELAAEIAVDFPEKKVTLVHKGPRLLE 119
>At5g22140.1 68418.m02578 pyridine nucleotide-disulphide
oxidoreductase family protein contains Pfam profile
PF00070 Pyridine nucleotide-disulphide oxidoreductase
domain
Length = 365
Score = 29.1 bits (62), Expect = 3.8
Identities = 18/42 (42%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +2
Query: 638 KNPENVLIVGGGDGGV--AREVAKHPQVKHIVLVEIDDRVIE 757
K+ +VLIVGGG GV A E+A K + LV R++E
Sbjct: 132 KSSGSVLIVGGGPSGVELAAEIAVDFPEKKVTLVHKGPRLLE 173
>At4g13330.1 68417.m02083 expressed protein
Length = 428
Score = 29.1 bits (62), Expect = 3.8
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = +2
Query: 653 VLIVGGGDGGVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPFMA 787
VL +G G G + +AKH + +VE+D VI S R + F A
Sbjct: 188 VLCIGHGGGSLPLFIAKHILGAVVDIVELDPLVISESVRAMGFPA 232
>At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 1012
Score = 29.1 bits (62), Expect = 3.8
Identities = 17/68 (25%), Positives = 29/68 (42%)
Frame = +2
Query: 422 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 601
WF D GG S+ V ++L + F + G VL++ G + F+Y
Sbjct: 369 WFDFMADTGDGGNSSYSVAKLLAQPSLRVPVANNFISLPRGNVLLIGGDLAYPNPSSFTY 428
Query: 602 QEMISFLP 625
++ + F P
Sbjct: 429 EKRL-FCP 435
>At3g04350.1 68416.m00460 expressed protein
Length = 567
Score = 29.1 bits (62), Expect = 3.8
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -2
Query: 586 FLSALYDSVQDKNLPQTGCIKNLD 515
F YD ++ +P GC+KNLD
Sbjct: 227 FFCCTYDLSSERTVPDIGCLKNLD 250
>At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyclin
3a [Arabidopsis thaliana] GI:509425; contains Pfam
profiles PF00134: Cyclin, N-terminal domain, PF02984:
Cyclin, C-terminal domain; identical to cDNA cyc3b mRNA
for cyclin 3b protein GI:728520
Length = 436
Score = 28.3 bits (60), Expect = 6.6
Identities = 28/112 (25%), Positives = 48/112 (42%), Gaps = 4/112 (3%)
Frame = +1
Query: 127 PQKIYLSTN---RSLSRVSVCHMYRIISLDIYIVIVKCEFSNVEFVRRAKRVFIFRARNT 297
P +YL+ N R LS S R+ L + +++ ++ E F F NT
Sbjct: 224 PDTLYLTVNLIDRFLSN-SYIERQRLQLLGVSCMLIASKYE--ELSAPGVEEFCFITANT 280
Query: 298 LFEREIVKTET*CQNLFNFRLQLPTWY*LLKQ-EKDGQTKNQLVYGIMRYVA 450
E++ E N +FRL +PT L++ K Q ++ + + Y+A
Sbjct: 281 YTRPEVLSMEIQILNFVHFRLSVPTTKTFLRRFIKAAQASYKVPFIELEYLA 332
>At1g14460.1 68414.m01715 DNA polymerase-related weak similarity to
DNA polymerase III holoenzyme tau subunit [Thermus
thermophilus] GI:2583049
Length = 1116
Score = 27.9 bits (59), Expect = 8.8
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -1
Query: 323 VFTISRSNNVFLARNIKTRFALLTNSTLLNSHFT 222
V +I+ S + L RN++ R LL+ + LLNS T
Sbjct: 925 VSSITNSIEMVLRRNVEVRIILLSETELLNSKQT 958
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,093,658
Number of Sequences: 28952
Number of extensions: 376296
Number of successful extensions: 884
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 861
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 884
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1921616800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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