Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_C01
(763 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 55 4e-08
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 50 2e-06
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 49 3e-06
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 49 4e-06
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 46 2e-05
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 46 3e-05
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 42 4e-04
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 40 0.002
At2g43400.1 68415.m05394 electron transfer flavoprotein-ubiquino... 30 1.9
At4g10370.1 68417.m01702 DC1 domain-containing protein contains ... 29 3.4
At3g61440.1 68416.m06881 cysteine synthase, putative / O-acetyls... 28 5.9
At2g13440.1 68415.m01483 glucose-inhibited division family A pro... 28 5.9
At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putat... 28 5.9
At4g37760.1 68417.m05345 squalene monooxygenase, putative / squa... 28 7.8
At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citr... 28 7.8
At3g05470.1 68416.m00599 formin homology 2 domain-containing pro... 28 7.8
At1g33135.1 68414.m04092 hypothetical protein 28 7.8
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 55.2 bits (127), Expect = 4e-08
Identities = 43/121 (35%), Positives = 63/121 (52%), Gaps = 4/121 (3%)
Frame = +2
Query: 317 SEYDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIVANYLQFTAANWGY 496
S YD+II+G GTAGC LA LS Q+ +LL+E G + + P + F AA
Sbjct: 44 SYYDYIIIGGGTAGCPLAATLS--QNASVLLLERGDSP---YNNPNITRLSAFGAALSDL 98
Query: 497 -KTKPSNKYCAGFENKQCNWPRGKVVGGSSVLNYMIYTRGSSVDYNN--WK-AMGNDGWG 664
++ PS ++ + E+ N R +V+GG S LN YTR + N W A+ N+ +
Sbjct: 99 SESSPSQRFVS--EDGVIN-ARARVLGGGSALNAGFYTRAGTKYVRNMGWDGALANESYQ 155
Query: 665 W 667
W
Sbjct: 156 W 156
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 50.0 bits (114), Expect = 2e-06
Identities = 39/122 (31%), Positives = 61/122 (50%), Gaps = 7/122 (5%)
Frame = +2
Query: 323 YDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIV--ANYLQFTAANWGY 496
+D+I+VG GTAGC LA LSE + +L++E G + P+V Y ++ N
Sbjct: 16 FDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSP---FGDPLVEERKYFGYSLLNTDE 70
Query: 497 KTKPSNKYCA--GFENKQCNWPRGKVVGGSSVLNYMIYTRGSS--VDYNNW-KAMGNDGW 661
+ + + + G EN RG+V+GGSS +N Y+R S V W K + + +
Sbjct: 71 YSSVAQSFTSVDGIENY-----RGRVLGGSSAINGGFYSRASDEFVKKAGWDKGLVQESY 125
Query: 662 GW 667
W
Sbjct: 126 KW 127
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 49.2 bits (112), Expect = 3e-06
Identities = 36/104 (34%), Positives = 53/104 (50%)
Frame = +2
Query: 323 YDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIVANYLQFTAANWGYKT 502
YD+IIVG GTAGC LA LS Q +++LL+E G ++ +L T +
Sbjct: 54 YDYIIVGGGTAGCPLAATLS--QSFRVLLLERGGVPYNRPNVMSHDGFLT-TLTDVNNFD 110
Query: 503 KPSNKYCAGFENKQCNWPRGKVVGGSSVLNYMIYTRGSSVDYNN 634
P+ + + E N RG+V+GGSS +N Y+R + N
Sbjct: 111 SPAQSFIS--EEGVPN-ARGRVLGGSSAINAGFYSRADKQFFEN 151
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 48.8 bits (111), Expect = 4e-06
Identities = 40/131 (30%), Positives = 66/131 (50%), Gaps = 4/131 (3%)
Frame = +2
Query: 323 YDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIVAN--YLQFTAANWGY 496
+D+I+VG GTAGC LA LSE + +L++E G + P+V + Y ++ N
Sbjct: 37 FDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSP---FGDPLVEDKKYYGYSLINTDE 91
Query: 497 KTKPSNKYCA--GFENKQCNWPRGKVVGGSSVLNYMIYTRGSSVDYNNWKAMGNDGWGWD 670
+ + + + G +N RG+V+GGSS +N Y+R S + + GW D
Sbjct: 92 YSSVAQSFTSVDGIKNH-----RGRVLGGSSAINGGFYSRASD------EFVKKAGWDKD 140
Query: 671 DVLPYFKKIEN 703
V +K +E+
Sbjct: 141 LVQESYKWVES 151
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 46.4 bits (105), Expect = 2e-05
Identities = 41/130 (31%), Positives = 65/130 (50%), Gaps = 1/130 (0%)
Frame = +2
Query: 314 LSEYDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIVANYLQFTAANWG 493
LS +D+II+G GTAGC LA LS Q+ +L++E G + D P + F AN
Sbjct: 43 LSHFDYIIIGGGTAGCALAATLS--QNATVLVLERGGSP---YDDPAATDIGNF--ANTL 95
Query: 494 YKTKPSNKYCAGFENKQCNW-PRGKVVGGSSVLNYMIYTRGSSVDYNNWKAMGNDGWGWD 670
P N + F ++ + R +V+GG +V+N Y+R D+ + GW D
Sbjct: 96 LNITP-NSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEE-DF-----VAEAGWERD 148
Query: 671 DVLPYFKKIE 700
+V ++ +E
Sbjct: 149 EVEAAYEWVE 158
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 46.0 bits (104), Expect = 3e-05
Identities = 37/112 (33%), Positives = 51/112 (45%), Gaps = 2/112 (1%)
Frame = +2
Query: 311 LLSEYDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIVANYLQFTAANW 490
+ + +D+II+G GT+GC LA LS Q+ +L++E G Y D P + F A
Sbjct: 42 MFARFDYIIIGGGTSGCALAATLS--QNASVLVLERG-GAPY--DNPTATDIENF--ATT 94
Query: 491 GYKTKPSNKYCAGFENKQCNWPRGKVVGGSSVLNYMIYTRGSS--VDYNNWK 640
T P + R +V+GG SVLN YTR V WK
Sbjct: 95 LSNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETEWK 146
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 41.9 bits (94), Expect = 4e-04
Identities = 30/101 (29%), Positives = 48/101 (47%)
Frame = +2
Query: 317 SEYDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIVANYLQFTAANWGY 496
S YD+I++G GTAGC LA LS Q++ +L++E G ++ + N+ A
Sbjct: 61 SSYDYIVIGGGTAGCPLAATLS--QNFSVLVLERGGVPFTNANVSFLRNFHIGLADISAS 118
Query: 497 KTKPSNKYCAGFENKQCNWPRGKVVGGSSVLNYMIYTRGSS 619
+ G N R +V+GG S +N Y+R +
Sbjct: 119 SASQAFVSTDGVYN-----ARARVLGGGSCINAGFYSRADA 154
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 39.5 bits (88), Expect = 0.002
Identities = 35/104 (33%), Positives = 52/104 (50%)
Frame = +2
Query: 317 SEYDFIIVGAGTAGCVLANRLSEIQDWKILLVEAGTNENYLMDIPIVANYLQFTAANWGY 496
+ +D+II+G GTAGC LA LS Q+ +L++E G + + N + T N
Sbjct: 71 ASFDYIIIGGGTAGCALAATLS--QNASVLVLERGGSPYENPTATDMGNSVN-TLLN--- 124
Query: 497 KTKPSNKYCAGFENKQCNWPRGKVVGGSSVLNYMIYTRGSSVDY 628
T S E+ N R +V+GG SV+N Y+R + DY
Sbjct: 125 NTPNSWSQLFISEDGVYN-TRPRVLGGGSVINGGFYSRAGN-DY 166
>At2g43400.1 68415.m05394 electron transfer flavoprotein-ubiquinone
oxidoreductase family protein contains Pfam profile:
PF05187 Electron transfer flavoprotein-ubiquinone
oxidoreductase
Length = 633
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/27 (51%), Positives = 17/27 (62%)
Frame = +2
Query: 320 EYDFIIVGAGTAGCVLANRLSEIQDWK 400
EYD +IVGAG AG A RL ++ K
Sbjct: 99 EYDVLIVGAGPAGLSAAIRLKQLSQEK 125
>At4g10370.1 68417.m01702 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 687
Score = 29.1 bits (62), Expect = 3.4
Identities = 13/29 (44%), Positives = 16/29 (55%), Gaps = 2/29 (6%)
Frame = -3
Query: 614 NPW--CISCN*VHLNLQLLCLWASYIVCF 534
N W C C ++NL L CL YI+CF
Sbjct: 494 NKWGRCQMCTYEYVNLNLNCLECDYILCF 522
>At3g61440.1 68416.m06881 cysteine synthase, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative identical to cysteine synthase
(EC 4.2.99.8) [Arabidopsis thaliana] GI:5824334;
contains Pfam profile PF00291: Pyridoxal-phosphate
dependent enzyme
Length = 368
Score = 28.3 bits (60), Expect = 5.9
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +2
Query: 224 QFANPINSLLNFVTEGTKQLDSEPPDQRNLLSEYDFIIVGAGTAGCV 364
QFANP N+ ++F T G P + L D ++G G+ G V
Sbjct: 191 QFANPANTQIHFDTTG-------PEIWEDTLGNVDIFVMGIGSGGTV 230
>At2g13440.1 68415.m01483 glucose-inhibited division family A
protein similar to GidA from Pseudomonas syringae
[GI:10764670]; contains Pfam profile PF01134 Glucose
inhibited division protein A
Length = 723
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +2
Query: 317 SEYDFIIVGAGTAGCVLA 370
S YD I+VGAG AGC A
Sbjct: 71 STYDVIVVGAGHAGCEAA 88
>At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putative /
citrate hydro-lyase/aconitase, putative nearly identical
to SP|P49608 Aconitate hydratase, cytoplasmic (EC
4.2.1.3) (Citrate hydro-lyase) (Aconitase) {Cucurbita
maxima}; contains Pfam profiles PF00330: Aconitase family
(aconitate hydratase), PF00694: Aconitase C-terminal
domain
Length = 990
Score = 28.3 bits (60), Expect = 5.9
Identities = 31/121 (25%), Positives = 45/121 (37%), Gaps = 10/121 (8%)
Frame = +2
Query: 422 TNENYLMDIPIVANYLQFTAANWGYKTKPSNKYCAGFE-NKQCNWPRGKVVG---GSSVL 589
T NYL P+V Y N ++T+P K G + + WP + + SSVL
Sbjct: 642 TRANYLASPPLVVAYALAGTVNIDFETEPIGKGKNGKDVFLRDIWPTTEEIAEVVQSSVL 701
Query: 590 NYMIYTRGSSVDYNN--WKAMG---NDGWGWDDVLPYFKKIENYNIPSFDDPXYHG-HDG 751
M S+ N W + N + WD Y + + + D P H D
Sbjct: 702 PDMFRATYESITKGNPMWNKLSVPENTLYSWDPNSTYIHEPPYFKDMTMDPPGPHNVKDA 761
Query: 752 Y 754
Y
Sbjct: 762 Y 762
>At4g37760.1 68417.m05345 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2), SP|O65403 (SE 2)
Length = 525
Score = 27.9 bits (59), Expect = 7.8
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +2
Query: 317 SEYDFIIVGAGTAGCVLANRLSE 385
++ D IIVGAG AG LA+ L +
Sbjct: 53 TDVDIIIVGAGVAGAALAHTLGK 75
>At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citrate
hydro-lyase / aconitase (ACO) identical to SP|Q42560
Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate
hydro-lyase) (Aconitase) {Arabidopsis thaliana};
contains Pfam profiles PF00330: Aconitase family
(aconitate hydratase), PF00694: Aconitase C-terminal
domain
Length = 898
Score = 27.9 bits (59), Expect = 7.8
Identities = 33/125 (26%), Positives = 47/125 (37%), Gaps = 14/125 (11%)
Frame = +2
Query: 422 TNENYLMDIPIVANYLQFTAANWGYKTKPSNKYCAGFENKQC----NWPRGKVVG---GS 580
T NYL P+V Y + ++T+P G + KQ WP K V S
Sbjct: 550 TRANYLASPPLVVAYALAGTVDIDFETQPIG---TGKDGKQIFFRDIWPSNKEVAEVVQS 606
Query: 581 SVL------NYMIYTRGSSVDYNNWKAMGNDGWGWDDVLPYFKKIENYNIPSFDDPXYHG 742
SVL Y T+G+S+ +N + WD Y + + + P HG
Sbjct: 607 SVLPDMFKATYEAITKGNSM-WNQLSVASGTLYEWDPKSTYIHEPPYFKGMTMSPPGPHG 665
Query: 743 -HDGY 754
D Y
Sbjct: 666 VKDAY 670
>At3g05470.1 68416.m00599 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 884
Score = 27.9 bits (59), Expect = 7.8
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +2
Query: 359 CVLANRLSEIQDWKILLVEAG 421
C++AN E+ DWK+L VE G
Sbjct: 25 CIVANA-KELDDWKVLTVENG 44
>At1g33135.1 68414.m04092 hypothetical protein
Length = 140
Score = 27.9 bits (59), Expect = 7.8
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = +1
Query: 346 WHRRVRTCQST*RNPRLENFTRRSGHKRKL-PNGYSYSG 459
W RRV T + RL+ TRR G + ++ +G S++G
Sbjct: 50 WRRRVATAAGEEESRRLQEKTRRDGEEEEVRSDGGSWNG 88
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,561,349
Number of Sequences: 28952
Number of extensions: 351456
Number of successful extensions: 891
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 858
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 886
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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