Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_B21
(740 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06580.1 68416.m00764 galactokinase (GAL1) identical to galac... 88 5e-18
At4g16130.1 68417.m02444 GHMP kinase family protein contains GHM... 72 5e-13
At3g42850.1 68416.m04489 galactokinase, putative contains some s... 69 2e-12
At3g10700.1 68416.m01288 GHMP kinase family protein contains Pfa... 43 2e-04
At5g27450.2 68418.m03277 mevalonate kinase (MK) identical to mev... 36 3e-04
At5g27450.1 68418.m03276 mevalonate kinase (MK) identical to mev... 36 3e-04
At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein... 31 0.61
At1g01220.1 68414.m00036 GHMP kinase-related contains similarity... 31 0.80
At5g55950.1 68418.m06978 transporter-related low similarity to U... 31 1.1
At5g14080.1 68418.m01647 pentatricopeptide (PPR) repeat-containi... 29 3.2
At5g18190.1 68418.m02135 protein kinase family protein contains ... 29 4.3
At2g27270.1 68415.m03277 expressed protein 29 4.3
At5g27030.1 68418.m03224 WD-40 repeat family protein contains 8 ... 28 5.7
At3g48620.1 68416.m05308 chloroplast outer membrane protein-rela... 28 5.7
At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 28 7.5
At1g75400.1 68414.m08759 expressed protein 28 7.5
At1g16780.1 68414.m02016 vacuolar-type H+-translocating inorgani... 28 7.5
At5g63450.1 68418.m07965 cytochrome P450, putative 27 9.9
At5g60990.1 68418.m07651 DEAD/DEAH box helicase, putative (RH10)... 27 9.9
At2g30390.1 68415.m03698 ferrochelatase II identical to Swiss-Pr... 27 9.9
>At3g06580.1 68416.m00764 galactokinase (GAL1) identical to
galactokinase (Galactose kinase) [Arabidopsis thaliana]
SWISS-PROT:Q9SEE5
Length = 496
Score = 88.2 bits (209), Expect = 5e-18
Identities = 76/225 (33%), Positives = 108/225 (48%), Gaps = 22/225 (9%)
Frame = +3
Query: 108 GEGVLLRQATDRFIV-------AYGRRPEVAAAAPGRVNLIGEHVDYCEGF-VLPVAIPF 263
GEG LL++AT RF V +G P++ A +PGRVNLIGEH+DY EG+ VLP+AI
Sbjct: 20 GEGSLLQEATQRFDVLKANFNDVFGASPQLFARSPGRVNLIGEHIDY-EGYSVLPMAIRQ 78
Query: 264 LTVV-VGSYNNTDVCRVITVLASGEELEASFPATIKAELRPGQPEWANYI---------- 410
T++ + + R+ V + + ++PA E+ +W +Y
Sbjct: 79 DTIIAIRKCEDQKQLRIANV--NDKYTMCTYPADPDQEIDLKNHKWGHYFICAYKGFHEY 136
Query: 411 ---KGVIANFPIKVNGFDIVVVSDVPMGSGVSSSAAIEVAVFTLLEGLTGRMVSLVEKAK 581
KGV P+ G D++V VP GSG+SSSAA + + + G E A+
Sbjct: 137 AKSKGVNLGSPV---GLDVLVDGIVPTGSGLSSSAAFVCSATIAIMAVFGHNFEKKELAQ 193
Query: 582 LCQKAEHDFPGMPCGIMDQYIVTMGKKDHALLIDCKSLEAKQVPI 716
L + E G G MDQ I M K A LID + A V +
Sbjct: 194 LTCECERHI-GTQSGGMDQAISIMAKTGFAELIDFNPVRATDVKL 237
>At4g16130.1 68417.m02444 GHMP kinase family protein contains GHMP
kinases putative ATP-binding protein domain,
Pfam:PF00288
Length = 1039
Score = 71.7 bits (168), Expect = 5e-13
Identities = 41/111 (36%), Positives = 60/111 (54%), Gaps = 5/111 (4%)
Frame = +3
Query: 381 PGQPEWANYIKGVIANFPIKV-----NGFDIVVVSDVPMGSGVSSSAAIEVAVFTLLEGL 545
P Q +WA Y+ G I I++ + ++V S VP G GVSSSAA+EVA + +
Sbjct: 657 PAQ-KWAAYVAGTILVLMIELGVRFEDSISLLVSSAVPEGKGVSSSAAVEVASMSAIAAA 715
Query: 546 TGRMVSLVEKAKLCQKAEHDFPGMPCGIMDQYIVTMGKKDHALLIDCKSLE 698
G + + A LCQK E+ G PCG+MDQ + G+ + L + C+ E
Sbjct: 716 HGLSIDPRDLAILCQKVENHIVGAPCGVMDQMTSSCGEANKLLAMICQPAE 766
>At3g42850.1 68416.m04489 galactokinase, putative contains some
similarity to galactokinase [Pasteurella multocida]
SWISS-PROT:P57899
Length = 964
Score = 69.3 bits (162), Expect = 2e-12
Identities = 41/111 (36%), Positives = 59/111 (53%), Gaps = 5/111 (4%)
Frame = +3
Query: 381 PGQPEWANYIKGVIANFPIKVN-----GFDIVVVSDVPMGSGVSSSAAIEVAVFTLLEGL 545
P Q +WA Y+ G I +++ I+V S VP G GVSSSA++EVA + +
Sbjct: 589 PSQ-KWAAYVAGTILVLMREMDVRFEDSISILVSSTVPEGKGVSSSASVEVATMSAVAAA 647
Query: 546 TGRMVSLVEKAKLCQKAEHDFPGMPCGIMDQYIVTMGKKDHALLIDCKSLE 698
G +S + A LCQK E+ G PCG+MDQ G+ + L + C+ E
Sbjct: 648 HGLEISPRDVALLCQKVENYVVGAPCGVMDQMASACGEANKLLAMICQPAE 698
>At3g10700.1 68416.m01288 GHMP kinase family protein contains Pfam
profile: PF00288 GHMP kinases putative ATP-binding
proteins
Length = 424
Score = 42.7 bits (96), Expect = 2e-04
Identities = 25/75 (33%), Positives = 39/75 (52%)
Frame = +3
Query: 486 SGVSSSAAIEVAVFTLLEGLTGRMVSLVEKAKLCQKAEHDFPGMPCGIMDQYIVTMGKKD 665
SG+SSSAA+ VA LE VS E + + E+ + G+ GI+DQ + +
Sbjct: 148 SGLSSSAAVGVAYLLALENANELTVSPTENIEYDRLIENGYLGLRNGILDQSAILLSNYG 207
Query: 666 HALLIDCKSLEAKQV 710
+DCK+L+ + V
Sbjct: 208 CLTYMDCKTLDHELV 222
>At5g27450.2 68418.m03277 mevalonate kinase (MK) identical to
mevalonate kinase [Arabidopsis thaliana]
SWISS-PROT:P46086
Length = 378
Score = 35.5 bits (78), Expect(2) = 3e-04
Identities = 24/87 (27%), Positives = 44/87 (50%), Gaps = 3/87 (3%)
Frame = +3
Query: 285 YNNTDVCRVITVLASGEELEASFPATIKAELRPGQPEWANYIKGVIANFPIKVNGFD--- 455
Y+++ +CR S E L+ S ++ + P + W + ++ GF+
Sbjct: 75 YDSSTLCRSTPASCSEETLK-SIAVLVEEQNLPKEKMWLSSGISTFLWLYTRIIGFNPAT 133
Query: 456 IVVVSDVPMGSGVSSSAAIEVAVFTLL 536
+V+ S++P GSG+ SSAA+ VA+ L
Sbjct: 134 VVINSELPYGSGLGSSAALCVALTAAL 160
Score = 26.2 bits (55), Expect(2) = 3e-04
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +3
Query: 171 EVAAAAPGRVNLIGEHVDYCEGFVLPVAIPFLTVV 275
EV A APG++ L GEH + AI T V
Sbjct: 2 EVKARAPGKIILAGEHAVVHGSTAVAAAIDLYTYV 36
>At5g27450.1 68418.m03276 mevalonate kinase (MK) identical to
mevalonate kinase [Arabidopsis thaliana]
SWISS-PROT:P46086
Length = 378
Score = 35.5 bits (78), Expect(2) = 3e-04
Identities = 24/87 (27%), Positives = 44/87 (50%), Gaps = 3/87 (3%)
Frame = +3
Query: 285 YNNTDVCRVITVLASGEELEASFPATIKAELRPGQPEWANYIKGVIANFPIKVNGFD--- 455
Y+++ +CR S E L+ S ++ + P + W + ++ GF+
Sbjct: 75 YDSSTLCRSTPASCSEETLK-SIAVLVEEQNLPKEKMWLSSGISTFLWLYTRIIGFNPAT 133
Query: 456 IVVVSDVPMGSGVSSSAAIEVAVFTLL 536
+V+ S++P GSG+ SSAA+ VA+ L
Sbjct: 134 VVINSELPYGSGLGSSAALCVALTAAL 160
Score = 26.2 bits (55), Expect(2) = 3e-04
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +3
Query: 171 EVAAAAPGRVNLIGEHVDYCEGFVLPVAIPFLTVV 275
EV A APG++ L GEH + AI T V
Sbjct: 2 EVKARAPGKIILAGEHAVVHGSTAVAAAIDLYTYV 36
>At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 474
Score = 31.5 bits (68), Expect = 0.61
Identities = 28/120 (23%), Positives = 52/120 (43%), Gaps = 6/120 (5%)
Frame = +3
Query: 315 TVLASGEELEAS-FPATIKAELRPGQPEWAN--YI---KGVIANFPIKVNGFDIVVVSDV 476
T+ GE +A FP T L P +W N Y+ K V+ P++ G DV
Sbjct: 348 TIQIKGEPSDAQQFPKTQCNLLNPYTLQWTNLTYLCVGKKVLRTKPLEEGG------DDV 401
Query: 477 PMGSGVSSSAAIEVAVFTLLEGLTGRMVSLVEKAKLCQKAEHDFPGMPCGIMDQYIVTMG 656
MG+ V + A ++ + +GL+ + ++ + + + +P +P +I +G
Sbjct: 402 RMGADVPADALVDRLLAGFFDGLSDGLPEVLHVSNQLVRTKESYPFLPFCASQSHIWKLG 461
>At1g01220.1 68414.m00036 GHMP kinase-related contains similarity to
L-fucose kinase [Homo sapiens] gi|21212956|emb|CAD29647
Length = 1055
Score = 31.1 bits (67), Expect = 0.80
Identities = 27/103 (26%), Positives = 48/103 (46%), Gaps = 6/103 (5%)
Frame = +3
Query: 234 GFVLPVAIPFL-TVVVGSYNNTDVCRVITVLA-SGEELEASFPATIKAELRPGQP----E 395
G+VL +AI ++ +G+ T I++ +G EL P +IK P +
Sbjct: 739 GYVLNMAITLEGSLPIGTIIETTNQMGISIQDDAGNELHIEDPISIKTPFEVNDPFRLVK 798
Query: 396 WANYIKGVIANFPIKVNGFDIVVVSDVPMGSGVSSSAAIEVAV 524
A + G++ + G I ++VP GSG+ +S+ + AV
Sbjct: 799 SALLVTGIVQENFVDSTGLAIKTWANVPRGSGLGTSSILAAAV 841
>At5g55950.1 68418.m06978 transporter-related low similarity to
UDP-sugar transporter [Drosophila melanogaster]
GI:14971008, UDP-glucuronic acid transporter [Homo
sapiens] GI:11463949
Length = 398
Score = 30.7 bits (66), Expect = 1.1
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 3/64 (4%)
Frame = -2
Query: 580 LAFSTKLTILPVSPSSSV---NTATSMAALLETPEPIGTSDTTTISKPFTFIGKLAITPL 410
LAF L++LP+SP S ++ S+ A++ + + S F + K+A+TP
Sbjct: 102 LAFFKSLSLLPMSPPSKTTPFSSLFSLGAVMAFASGLANTSLKHNSVGFYQMAKIAVTPT 161
Query: 409 I*LA 398
I LA
Sbjct: 162 IVLA 165
>At5g14080.1 68418.m01647 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 634
Score = 29.1 bits (62), Expect = 3.2
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 6/73 (8%)
Frame = -1
Query: 374 LCLDGSRETGLQLLTGRQHCDHATDICVVVRSHDDGKE-----RYCHW*NEAF-AIVNVF 213
LC +G QLL R+H +H V+++ D KE R+ W E ++V+
Sbjct: 548 LCSNGHSGEASQLLREREHLEHTGAHVVLLKCVADAKEVEIGIRHMQWIKEVSPSLVHTI 607
Query: 212 SDEVDASRRRCSD 174
S ++ AS SD
Sbjct: 608 SSDLLASFCSSSD 620
>At5g18190.1 68418.m02135 protein kinase family protein contains
Pfam domains, PF00069: Protein kinase domain
Length = 691
Score = 28.7 bits (61), Expect = 4.3
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 454 ILLSYPTCRWVLVFPAAPPLKWQYSRC*KDSR 549
I + P C+W+ V+ A P+K +Y DSR
Sbjct: 446 IRIGSPACQWISVYNARRPMKQRYHYNVADSR 477
>At2g27270.1 68415.m03277 expressed protein
Length = 231
Score = 28.7 bits (61), Expect = 4.3
Identities = 19/69 (27%), Positives = 32/69 (46%)
Frame = -2
Query: 283 DPTTTVRNGIATGRTKPSQ*STCSPMRLTRPGAAAATSGRLPYATMNLSVACRNNTPSPF 104
D T +NG T R +P++ T GAAA+ R+ Y+ + ++ T F
Sbjct: 5 DEATMKKNGPTTKREEPTKKKT----EAKEEGAAASKHSRVVYSDKSRCLSKNGKTIIYF 60
Query: 103 GIASQFIIL 77
G+ + I+L
Sbjct: 61 GVPAALILL 69
>At5g27030.1 68418.m03224 WD-40 repeat family protein contains 8
WD-40 repeats (PF00400) (2 weak)
Length = 1108
Score = 28.3 bits (60), Expect = 5.7
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 1/59 (1%)
Frame = -2
Query: 334 SPDANTVITRQTSVLLYDPTT-TVRNGIATGRTKPSQ*STCSPMRLTRPGAAAATSGRL 161
+PD T+ T T L P + N T TKP+ + P PG AAA +G L
Sbjct: 192 NPDIKTLFTDHTCTLPNGPLAPSAVNQPVTTLTKPAAYPSLGPHVPFPPGPAAANAGAL 250
>At3g48620.1 68416.m05308 chloroplast outer membrane protein-related
weak similarity to chloroplastic outer envelope membrane
protein (OEP75) [Pisum sativum] GI:633607
Length = 328
Score = 28.3 bits (60), Expect = 5.7
Identities = 16/45 (35%), Positives = 20/45 (44%)
Frame = -2
Query: 223 STCSPMRLTRPGAAAATSGRLPYATMNLSVACRNNTPSPFGIASQ 89
STC +RLT SGR+ Y N S+ C NT + Q
Sbjct: 37 STCMCVRLTMTDGRYQRSGRISYNMQN-SLLCSGNTHDSMVVLKQ 80
>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
PF05064 Nsp1-like C-terminal region
Length = 739
Score = 27.9 bits (59), Expect = 7.5
Identities = 45/170 (26%), Positives = 70/170 (41%), Gaps = 21/170 (12%)
Frame = -2
Query: 538 SSSVNTATSMAALLETPEPIGTSDTTTISKPFTFIGKLAITPL------I*LAHSGWPGR 377
SS+ +TAT ++L P +S T S PF TPL + A S
Sbjct: 146 SSASSTATPSSSLFGAP---ASSAATPSSSPFGAAPASGSTPLFGSSPSLFSAPSSASAS 202
Query: 376 NSALMVAGKLASNSS------PDANTVITRQTSVLLYDPTTTVRNGIATGRTKP----SQ 227
NS+L A A+ S+ P + T T SV P ++ ATG + S
Sbjct: 203 NSSLFGASSSAATSTSPLFGAPSSATGATPSFSVASSAPGSSSSIFGATGSSPSFSVASS 262
Query: 226 *STCSPMRLTRPGAA-----AATSGRLPYATMNLSVACRNNTPSPFGIAS 92
S SP G++ ++++G P + S ++PSPFG+++
Sbjct: 263 ASGSSPSIFGATGSSPFFGSSSSAGSTPSLFASSSSGATTSSPSPFGVST 312
>At1g75400.1 68414.m08759 expressed protein
Length = 455
Score = 27.9 bits (59), Expect = 7.5
Identities = 16/62 (25%), Positives = 32/62 (51%)
Frame = +1
Query: 169 RKSLQRRRDASTSSENTLTIAKASFYQWQYRSLPSSWDRTTTQMSVA*SQCWRPVRSWRP 348
R+ + +AS SS+++ + SF +W + S+ S W++ +++ S W R+ R
Sbjct: 386 REGKALKLEASCSSKSS---SNKSFLKWHFASISSKWNKPSSKDSALKKGFWSRHRNNRS 442
Query: 349 VS 354
S
Sbjct: 443 SS 444
>At1g16780.1 68414.m02016 vacuolar-type H+-translocating inorganic
pyrophosphatase, putative similar to vacuolar-type
H+-translocating inorganic pyrophosphatase GI:6901676
from [Arabidopsis thaliana]
Length = 802
Score = 27.9 bits (59), Expect = 7.5
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = +1
Query: 448 VSILLSYPTCRWVLVFPAAPPLKWQYSRC*KDSREGWL 561
+S +LS +WVL PP Q S +D EG+L
Sbjct: 77 ISFMLSVYLTKWVLAKDEGPPEMVQISDAIRDGAEGFL 114
>At5g63450.1 68418.m07965 cytochrome P450, putative
Length = 510
Score = 27.5 bits (58), Expect = 9.9
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 452 ETVHFYRKISNNSFDIISPFRLAWS*LCLDGSR 354
ETV F + +FD++ L W CLD +R
Sbjct: 173 ETVDFQEVLKRFAFDVVCKVSLGWDPDCLDLTR 205
>At5g60990.1 68418.m07651 DEAD/DEAH box helicase, putative (RH10)
probable replication protein A1, Oryza sativa,
EMBL:AF009179
Length = 456
Score = 27.5 bits (58), Expect = 9.9
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +3
Query: 150 VAYGRRPEVAAAAPGRVNLIGEHVDYCEGFVL 245
+A G+RP V A PGR + +H+ +GF L
Sbjct: 134 IALGKRPHVIVATPGR---LWDHMSDTKGFSL 162
>At2g30390.1 68415.m03698 ferrochelatase II identical to
Swiss-Prot:O04921 ferrochelatase II, chloroplast
precursor (EC 4.99.1.1) (Protoheme ferro-lyase) (Heme
synthetase) [Arabidopsis thaliana]
Length = 512
Score = 27.5 bits (58), Expect = 9.9
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = -2
Query: 568 TKLTILPVSPSSSVNTATSMAALLE 494
TKL +LP+ P S++T+ S LLE
Sbjct: 217 TKLVVLPLYPQFSISTSGSSLRLLE 241
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,681,912
Number of Sequences: 28952
Number of extensions: 399921
Number of successful extensions: 1113
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1071
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1110
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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