Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_B11
(775 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 134 1e-32
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 85 8e-18
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 59 8e-10
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 44 2e-05
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|... 28 1.7
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 3.0
SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1... 27 3.0
SPBP23A10.02 |||conserved fungal protein|Schizosaccharomyces pom... 27 3.9
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 27 3.9
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 5.2
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 9.1
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 25 9.1
>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 199
Score = 134 bits (325), Expect = 1e-32
Identities = 65/187 (34%), Positives = 106/187 (56%)
Frame = +3
Query: 153 VSNAYSYTVTVDAHAEECFYENVEADTKLGLSFEIAEGGFLDIDVIILGPDGNVIHKAER 332
+S + + +T+ H ECFYEN+ + ++ ++++ GG + + I P G ++H+
Sbjct: 16 ISVVFGHGITLKPHQRECFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVP 75
Query: 333 ESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNENEADHNKLEDMI 512
S Y+F+ GKY YCF N K V+FN+ +E+ +N L +
Sbjct: 76 NSMAQYSFTVKNPGKYMYCFYNDALDGESKEVLFNVH---GVIYISDEDLDANNPLLGKV 132
Query: 513 KELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVMTIGQVYYLKRF 692
++L T+ +KH+QEY R+RIHR+ ESTN RV WSI + +L+ + + Q++YLKR
Sbjct: 133 RQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSVCVFQIFYLKRL 192
Query: 693 FEVQRVV 713
FEV+RVV
Sbjct: 193 FEVKRVV 199
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 85.4 bits (202), Expect = 8e-18
Identities = 44/195 (22%), Positives = 95/195 (48%), Gaps = 5/195 (2%)
Frame = +3
Query: 129 LVVTLLNLVSNAYSYTVTVDAHAEECFY-ENVEADTKLGLSFEIAEGGFLDIDVIILGPD 305
+ + LL +++A T ++ ++C+Y ++ K ++ + GG D+D +I P
Sbjct: 9 ITLFLLASLASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPS 68
Query: 306 GNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNL----EIGEPQTKSGN 473
+ ++ F+ G+Y +CF N MS+ T K+V + E+ P
Sbjct: 69 QKTVALGKKRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDE 128
Query: 474 ENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLL 653
+ + ++ + E++T L I Q Y + R+ + S +ST +R+ +S+ E+ +++
Sbjct: 129 AKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESIMVV 188
Query: 654 VMTIGQVYYLKRFFE 698
++ QV+ +K FF+
Sbjct: 189 ALSALQVFIVKTFFK 203
>SPBC16E9.09c |||COPII vesicle coat component
Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 58.8 bits (136), Expect = 8e-10
Identities = 38/140 (27%), Positives = 65/140 (46%), Gaps = 3/140 (2%)
Frame = +3
Query: 303 DGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGN--- 473
+ ++I+K + S+ F+ G + C+ + L + N
Sbjct: 76 NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135
Query: 474 ENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLL 653
+E + L D + L T L +I +Q + R+ R +ES NSRVV W+I + VLL
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVVLL 195
Query: 654 VMTIGQVYYLKRFFEVQRVV 713
V I Q+ +L+RFF +++V
Sbjct: 196 VTCIWQLSHLQRFFVKEKLV 215
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 44.4 bits (100), Expect = 2e-05
Identities = 27/78 (34%), Positives = 41/78 (52%)
Frame = +3
Query: 480 EADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVM 659
EAD + D I+E IK Y+Q R+ R+ NESTN RV ++ L V+
Sbjct: 145 EADIRRARDFIEE-------IKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVL 197
Query: 660 TIGQVYYLKRFFEVQRVV 713
I Q+ YL+ FF+ + ++
Sbjct: 198 VIWQILYLRSFFQRKHLI 215
>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
Mts4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.9 bits (59), Expect = 1.7
Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 3/48 (6%)
Frame = -1
Query: 667 PIVMTSSTEASNID---HITTREFVLSFIERWIRSRTCIYS*SCLIVL 533
P +T + EA ID + E V+ F+E SR C+Y SC+ +L
Sbjct: 195 PFFLTHNAEADAIDLLQELGAIEKVVPFVELDNASRVCLYITSCVNLL 242
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 27.1 bits (57), Expect = 3.0
Identities = 13/29 (44%), Positives = 21/29 (72%), Gaps = 2/29 (6%)
Frame = +3
Query: 615 VVMWSIFEASVLLVM-TIGQVYYL-KRFF 695
+V+W +F+ VLLV T+G ++L K+FF
Sbjct: 560 LVLWMLFQVLVLLVQDTLGPRFFLPKKFF 588
>SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1023
Score = 27.1 bits (57), Expect = 3.0
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = -2
Query: 312 RCHQGLVLSHQYLRNHLLLFQT 247
R +++H YLR+H+ LF+T
Sbjct: 762 RVRMSAIMNHAYLRSHVSLFET 783
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 2 MLRGPGHKLKHTHAQT*KTKIKQK 73
+LR P HK+KH +T ++ +K K
Sbjct: 878 LLRRPKHKVKHAKVETRESPMKNK 901
>SPBP23A10.02 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 132
Score = 26.6 bits (56), Expect = 3.9
Identities = 11/42 (26%), Positives = 23/42 (54%)
Frame = +3
Query: 465 SGNENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRS 590
S N+N +++ ++ D + ATT +T +E ++ R + S
Sbjct: 91 SNNQNPSNNKEMSDKESDSATTTQTFSVPEELLRARTTANNS 132
>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 26.6 bits (56), Expect = 3.9
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +1
Query: 301 LMATSFIKQRGSHLVFIHFLLQLQGSILIASVIKCHP 411
+M T +K G + +HF+ + G+ L ++ KC P
Sbjct: 170 VMVTEALKPYGQENLELHFVSNIDGTHLAEALKKCDP 206
>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1
Query: 166 YALLTRFNKVTTSHCDILKYFILLNV 89
YA+L N +T++ CD Y +L+ V
Sbjct: 1026 YAILNILNLLTSNECDEFDYSVLMKV 1051
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 25.4 bits (53), Expect = 9.1
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +3
Query: 486 DHNKLEDMIKELATTLRTIK 545
DH+KL+D +++ A+TL IK
Sbjct: 723 DHSKLDDDLEKFASTLDPIK 742
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 25.4 bits (53), Expect = 9.1
Identities = 8/37 (21%), Positives = 21/37 (56%)
Frame = +3
Query: 477 NEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHR 587
N+A + K +D I++ +T++ +Y+ ++H+
Sbjct: 9 NDAKYRKYQDAIRKCIALFQTVEQWPDYISFLTKLHK 45
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,160,423
Number of Sequences: 5004
Number of extensions: 64970
Number of successful extensions: 180
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 373338084
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -