Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_A23
(845 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g05010.1 68418.m00532 clathrin adaptor complexes medium subun... 197 9e-51
At2g17380.1 68415.m02007 clathrin assembly protein AP19 identica... 36 0.045
At4g35410.2 68417.m05030 clathrin adaptor complex small chain fa... 34 0.14
At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun... 30 1.7
At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun... 30 1.7
At5g13760.1 68418.m01604 expressed protein similar to unknown pr... 30 2.2
At2g40020.1 68415.m04918 expressed protein 29 2.9
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 29 2.9
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 29 2.9
At4g35410.1 68417.m05029 clathrin adaptor complex small chain fa... 29 3.9
At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 5.1
At2g36560.1 68415.m04484 DNA-binding protein-related contains Pf... 28 6.8
At1g66330.2 68414.m07533 senescence-associated family protein si... 28 6.8
At1g66330.1 68414.m07532 senescence-associated family protein si... 28 6.8
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun... 28 6.8
At1g53570.2 68414.m06081 mitogen-activated protein kinase kinase... 28 9.0
At1g53570.1 68414.m06080 mitogen-activated protein kinase kinase... 28 9.0
>At5g05010.1 68418.m00532 clathrin adaptor complexes medium
subunit-related contains pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 527
Score = 197 bits (480), Expect = 9e-51
Identities = 89/177 (50%), Positives = 131/177 (74%)
Frame = +3
Query: 96 MVLIAATVCTKSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQP 275
MV++AA + KSGK +VSR +V+M++ RIEGLLAAFPKL+ G+QHT++ETE+VRYVYQP
Sbjct: 1 MVVLAAAIVVKSGKVIVSRHYVDMSRIRIEGLLAAFPKLVGMGKQHTYIETENVRYVYQP 60
Query: 276 LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALG 455
++ L++LL+TTK SNILEDL TL L S++VPEY + L E + +F L+FAFDE+++LG
Sbjct: 61 IEALFLLLVTTKQSNILEDLATLTLLSKLVPEYSMSLDEEGISRASFELIFAFDEVISLG 120
Query: 456 YRESVNLAQVRSFVEMDSHEEKIYQAVRQTQXXXXXXXXXXXXXXLQRERLEAAKRG 626
++ESV +AQV+ + EM+SHEEK+++ V Q++ + + ++E K G
Sbjct: 121 HKESVTVAQVKQYCEMESHEEKLHKLVMQSKINDTKDVMKRKANEIDKSKIEKNKPG 177
>At2g17380.1 68415.m02007 clathrin assembly protein AP19 identical
to clathrin assembly protein AP19 GI:2231698 from
[Arabidopsis thaliana]
Length = 161
Score = 35.5 bits (78), Expect = 0.045
Identities = 19/82 (23%), Positives = 36/82 (43%)
Frame = +3
Query: 210 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 389
L G + F+E + VY+ LY + +A N LE LE + + ++ Y +
Sbjct: 40 LNRGPKLCNFIEWRGYKVVYKRYASLYFCMCIDEADNELEVLEIIHHYVEILDRYFGSVC 99
Query: 390 ETEVLNQAFNLLFAFDEIVALG 455
E +++ + DE++ G
Sbjct: 100 ELDLIFNFHKAYYILDELLIAG 121
>At4g35410.2 68417.m05030 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 162
Score = 33.9 bits (74), Expect = 0.14
Identities = 19/82 (23%), Positives = 35/82 (42%)
Frame = +3
Query: 210 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 389
L G + FVE + VY+ LY + + N LE LE + + ++ Y +
Sbjct: 40 LNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEIIHHYVEILDRYFGSVC 99
Query: 390 ETEVLNQAFNLLFAFDEIVALG 455
E +++ + DE++ G
Sbjct: 100 ELDLIFNFHKAYYILDELLIAG 121
>At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 451
Score = 30.3 bits (65), Expect = 1.7
Identities = 20/84 (23%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
Frame = +3
Query: 249 ESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLF 428
+ V Y + + LY + T + LE L+ +RV+ +Y L E + + F L++
Sbjct: 55 DGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNE-DSFRKNFVLVY 113
Query: 429 AF-DEIVALGYRESVNLAQVRSFV 497
DE++ GY ++ + ++S++
Sbjct: 114 ELLDEVIDFGYVQTTSTEVLKSYI 137
>At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 380
Score = 30.3 bits (65), Expect = 1.7
Identities = 20/84 (23%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
Frame = +3
Query: 249 ESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLF 428
+ V Y + + LY + T + LE L+ +RV+ +Y L E + + F L++
Sbjct: 55 DGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNE-DSFRKNFVLVY 113
Query: 429 AF-DEIVALGYRESVNLAQVRSFV 497
DE++ GY ++ + ++S++
Sbjct: 114 ELLDEVIDFGYVQTTSTEVLKSYI 137
>At5g13760.1 68418.m01604 expressed protein similar to unknown
protein (gb AAF63775.1)
Length = 569
Score = 29.9 bits (64), Expect = 2.2
Identities = 13/41 (31%), Positives = 23/41 (56%)
Frame = +3
Query: 336 ETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGY 458
+T +F+ + L T+ N+ F LLF F ++VA+G+
Sbjct: 118 QTRSIFTPTPQQTLASLNSTKYTNKFFLLLFIFHKVVAIGF 158
>At2g40020.1 68415.m04918 expressed protein
Length = 228
Score = 29.5 bits (63), Expect = 2.9
Identities = 12/23 (52%), Positives = 20/23 (86%), Gaps = 1/23 (4%)
Frame = -2
Query: 703 DSDGDDEDIVEL-PKPLPKDICE 638
DSDGDD++I++L P+PL +++ E
Sbjct: 187 DSDGDDDEIIQLTPEPLCEELQE 209
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 29.5 bits (63), Expect = 2.9
Identities = 20/98 (20%), Positives = 43/98 (43%)
Frame = +3
Query: 210 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 389
L +Q +FVE + + VY+ L+ ++ N L LE + L + ++ +
Sbjct: 41 LARNDQQCSFVEHRNYKIVYRRYASLFFMVGVDDDENELAILEFIHLLVETMDKHFGNVC 100
Query: 390 ETEVLNQAFNLLFAFDEIVALGYRESVNLAQVRSFVEM 503
E +++ F +E+V G + A + S +++
Sbjct: 101 ELDIMFHLEKAHFMLEEMVMNGCIVETSKANILSPIQL 138
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 29.5 bits (63), Expect = 2.9
Identities = 28/136 (20%), Positives = 60/136 (44%), Gaps = 3/136 (2%)
Frame = +3
Query: 132 GKALVSRQFV-EMTKARIEGLLAAFPKLMTGGRQHTFVETES-VRYVYQPLDKLYMLLIT 305
G+ LV R + ++T A+ E + + + V ++ V Y++ +Y+++ +
Sbjct: 15 GRVLVWRDYRGDVTAAQAERFFTKLIETEGDSQSNDPVAYDNGVTYMFVQHSNIYLMIAS 74
Query: 306 TKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAF-DEIVALGYRESVNLAQ 482
+ N L L V Y +L E E L F +++ DE++ GY +
Sbjct: 75 RQNCNAASLLFFLHRVVDVFKHYFEELEE-ESLRDNFVVVYELLDEMMDFGYPQFTEARI 133
Query: 483 VRSFVEMDSHEEKIYQ 530
+ F++ D++ ++ Q
Sbjct: 134 LSEFIKTDAYRMEVTQ 149
>At4g35410.1 68417.m05029 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 124
Score = 29.1 bits (62), Expect = 3.9
Identities = 16/65 (24%), Positives = 29/65 (44%)
Frame = +3
Query: 210 LMTGGRQHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLT 389
L G + FVE + VY+ LY + + N LE LE + + ++ Y +
Sbjct: 40 LNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEIIHHYVEILDRYFGSVC 99
Query: 390 ETEVL 404
E +++
Sbjct: 100 ELDLI 104
>At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to flavanone 3-hydroxylase
[Persea americana][GI:727410]; contains PF03171
2OG-Fe(II) oxygenase superfamily domain
Length = 341
Score = 28.7 bits (61), Expect = 5.1
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = -1
Query: 404 QDFSFCELYTIFWYHSAEESQCLQIF 327
+DF++ E Y FW + ++ CL+ F
Sbjct: 313 KDFTYAEYYKKFWSRNLDQEHCLENF 338
>At2g36560.1 68415.m04484 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 574
Score = 28.3 bits (60), Expect = 6.8
Identities = 15/58 (25%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = -2
Query: 829 SPSXRSRDTNESASV---PRLPSFMAERPTVRVSLAGVVGIFSAIDSDGDDEDIVELP 665
S S +D + +A P PSF+A T +V ++G + + + + D++ LP
Sbjct: 390 SHSQEKKDESNNAMAVFSPTAPSFLASSSTEQVQQPDMIGPYHSRQRNDESNDVILLP 447
>At1g66330.2 68414.m07533 senescence-associated family protein
similar to senescence-associated protein (GI:12836895)
[Ipomoea batatas]
Length = 417
Score = 28.3 bits (60), Expect = 6.8
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = -2
Query: 397 SVSVSCTQYSGTTLLKSLNVSRSSRMLLAFV 305
+++VS + L+KS+N SRS R+LLA+V
Sbjct: 2 ALNVSKVVPNSPILVKSVNASRSRRVLLAYV 32
>At1g66330.1 68414.m07532 senescence-associated family protein
similar to senescence-associated protein (GI:12836895)
[Ipomoea batatas]
Length = 417
Score = 28.3 bits (60), Expect = 6.8
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = -2
Query: 397 SVSVSCTQYSGTTLLKSLNVSRSSRMLLAFV 305
+++VS + L+KS+N SRS R+LLA+V
Sbjct: 2 ALNVSKVVPNSPILVKSVNASRSRRVLLAYV 32
>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 28.3 bits (60), Expect = 6.8
Identities = 20/93 (21%), Positives = 42/93 (45%), Gaps = 1/93 (1%)
Frame = +3
Query: 255 VRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAF 434
V Y++ +Y+++ + + N L L V Y +L E E L F +++
Sbjct: 58 VTYMFVQHSNVYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEE-ESLRDNFVVVYEL 116
Query: 435 -DEIVALGYRESVNLAQVRSFVEMDSHEEKIYQ 530
DE++ GY + + F++ D++ ++ Q
Sbjct: 117 LDEMMDFGYPQYTEARILSEFIKTDAYRMEVTQ 149
>At1g53570.2 68414.m06081 mitogen-activated protein kinase kinase
kinase (MAPKKK), putative (MAP3Ka) identical to MEK
kinase (MAP3Ka)[Arabidopsis thaliana]
gi|4204912|gb|AAD10848
Length = 608
Score = 27.9 bits (59), Expect = 9.0
Identities = 19/54 (35%), Positives = 22/54 (40%), Gaps = 2/54 (3%)
Frame = -2
Query: 811 RDTNESASV--PRLPSFMAERPTVRVSLAGVVGIFSAIDSDGDDEDIVELPKPL 656
RD SA V P L P AG FS DSD ++ LP+PL
Sbjct: 25 RDIKSSAVVVDPPLTPTRGGTPRCSREFAGASSAFSGFDSDSTEKKGHPLPRPL 78
>At1g53570.1 68414.m06080 mitogen-activated protein kinase kinase
kinase (MAPKKK), putative (MAP3Ka) identical to MEK
kinase (MAP3Ka)[Arabidopsis thaliana]
gi|4204912|gb|AAD10848
Length = 609
Score = 27.9 bits (59), Expect = 9.0
Identities = 19/54 (35%), Positives = 22/54 (40%), Gaps = 2/54 (3%)
Frame = -2
Query: 811 RDTNESASV--PRLPSFMAERPTVRVSLAGVVGIFSAIDSDGDDEDIVELPKPL 656
RD SA V P L P AG FS DSD ++ LP+PL
Sbjct: 25 RDIKSSAVVVDPPLTPTRGGTPRCSREFAGASSAFSGFDSDSTEKKGHPLPRPL 78
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,253,666
Number of Sequences: 28952
Number of extensions: 372107
Number of successful extensions: 1076
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1048
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1076
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1960634400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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