Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P02_F_A17
(732 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_3407| Best HMM Match : TPX2 (HMM E-Value=2.9e-09) 38 0.006
SB_23388| Best HMM Match : ParBc (HMM E-Value=0.3) 35 0.078
SB_28981| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.10
SB_46755| Best HMM Match : zf-C2H2 (HMM E-Value=1.09301e-43) 33 0.24
SB_5831| Best HMM Match : TolA (HMM E-Value=0.0037) 32 0.42
SB_20689| Best HMM Match : Gelsolin (HMM E-Value=0.00029) 32 0.55
SB_41| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.96
SB_42146| Best HMM Match : GYF (HMM E-Value=5.7e-15) 31 1.3
SB_59580| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.7
SB_11898| Best HMM Match : DMP1 (HMM E-Value=1.6) 30 1.7
SB_30472| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.2
SB_49860| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.9
SB_26233| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.9
SB_54309| Best HMM Match : SH2 (HMM E-Value=5.1e-17) 29 2.9
SB_13201| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.9
SB_46756| Best HMM Match : zf-C2H2 (HMM E-Value=4.60046e-42) 29 3.9
SB_46344| Best HMM Match : ig (HMM E-Value=0.0082) 29 5.1
SB_23345| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.1
SB_49663| Best HMM Match : TolA (HMM E-Value=0.059) 29 5.1
SB_42720| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.1
SB_15709| Best HMM Match : CAP_GLY (HMM E-Value=0) 29 5.1
SB_53860| Best HMM Match : fn3 (HMM E-Value=3.6e-07) 28 6.8
SB_47823| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.8
SB_37749| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.8
SB_30292| Best HMM Match : fn3 (HMM E-Value=1.2e-12) 28 6.8
SB_2413| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.8
SB_48387| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.8
SB_47629| Best HMM Match : Vicilin_N (HMM E-Value=1.1) 28 6.8
SB_22747| Best HMM Match : 7tm_1 (HMM E-Value=3.1e-06) 28 9.0
SB_53672| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 9.0
SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0) 28 9.0
>SB_3407| Best HMM Match : TPX2 (HMM E-Value=2.9e-09)
Length = 787
Score = 38.3 bits (85), Expect = 0.006
Identities = 23/61 (37%), Positives = 36/61 (59%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 654
+ ++ L + EKK Q ML+ K A+K P F ++ +EN G A+ N +++LEEEK
Sbjct: 607 QRNKEALAKKEKKIQEMLEEEKKATKFEP-FNLE--TENRGSVKAEKWLNSVQQELEEEK 663
Query: 655 K 657
K
Sbjct: 664 K 664
>SB_23388| Best HMM Match : ParBc (HMM E-Value=0.3)
Length = 1168
Score = 34.7 bits (76), Expect = 0.078
Identities = 17/61 (27%), Positives = 36/61 (59%)
Frame = +1
Query: 469 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 648
++EEK++RL+ E+ + ++ +A K+ASK+ + T + +S N ++ K ++E
Sbjct: 427 EVEEKKRRLQRYERLQSSLNEAYKEASKSSVDGT-RDESRNEEITQTSCNETSGKTPIKE 485
Query: 649 E 651
E
Sbjct: 486 E 486
>SB_28981| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 655
Score = 34.3 bits (75), Expect = 0.10
Identities = 15/25 (60%), Positives = 21/25 (84%)
Frame = +1
Query: 472 IEEKRQRLEEAEKKRQAMLQAMKDA 546
+EE+R+RLE EK+RQA QAM++A
Sbjct: 319 LEEERKRLENLEKERQAAQQAMQEA 343
>SB_46755| Best HMM Match : zf-C2H2 (HMM E-Value=1.09301e-43)
Length = 1806
Score = 33.1 bits (72), Expect = 0.24
Identities = 24/83 (28%), Positives = 43/83 (51%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 654
EE+RQ+ EEA K+R+ L A K++SK+ +K E G + + ++ TK + E
Sbjct: 1344 EERRQKREEARKRREEKL-AKKESSKSSNK---RKSKERSGNPSFKRIKSTTKGRETEVT 1399
Query: 655 KISLSIRIKPLTIEGLSVDKLRQ 723
K S + + +S+ + R+
Sbjct: 1400 KDSPVVESSDKSCSDMSIKRSRK 1422
>SB_5831| Best HMM Match : TolA (HMM E-Value=0.0037)
Length = 703
Score = 32.3 bits (70), Expect = 0.42
Identities = 21/59 (35%), Positives = 33/59 (55%)
Frame = +1
Query: 472 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 648
+EE+R+R EEAEKKR+ + ++ + + KK E L QLER K +++ E
Sbjct: 252 MEEERKRKEEAEKKREEEERKRREEEEAAQKW---KKEEL--LRQQQLEREKEEQERAE 305
>SB_20689| Best HMM Match : Gelsolin (HMM E-Value=0.00029)
Length = 1866
Score = 31.9 bits (69), Expect = 0.55
Identities = 25/84 (29%), Positives = 41/84 (48%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 654
++KR+R E+ K+RQ L+ K+ + I+K+ Q ER + KE+ E++K
Sbjct: 914 KDKREREEKERKRRQQQLE--KEKKEKEKKLLIEKEKRE---KEKQKERLREKEEKEKQK 968
Query: 655 KISLSIRIKPLTIEGLSVDKLRQK 726
+ R K L DKL +K
Sbjct: 969 EAE---RAKKEKERLLQEDKLHEK 989
>SB_41| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 306
Score = 31.1 bits (67), Expect = 0.96
Identities = 17/43 (39%), Positives = 19/43 (44%)
Frame = -3
Query: 730 GPSVGVCRRRDPRWSAA*CGWTGRSSSPLPAAPWSCCAPAGHC 602
G V C R D A CG TG+ S P +CCA A C
Sbjct: 173 GQDVNRCHR-DAAIVAKGCGCTGQYSQTCTQGPGACCATAESC 214
>SB_42146| Best HMM Match : GYF (HMM E-Value=5.7e-15)
Length = 924
Score = 30.7 bits (66), Expect = 1.3
Identities = 16/63 (25%), Positives = 34/63 (53%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 654
EE+R++ EEAE++R+ + K + +QK+ + Q ++ K Q EE++
Sbjct: 636 EERRRQQEEAERQRREQEEVFKQQEQQRQQLELQKRQQ-----EQQRQQELQKRQQEEKQ 690
Query: 655 KIS 663
+++
Sbjct: 691 RMA 693
>SB_59580| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 999
Score = 30.3 bits (65), Expect = 1.7
Identities = 20/66 (30%), Positives = 35/66 (53%), Gaps = 4/66 (6%)
Frame = +1
Query: 472 IEEKRQRLE----EAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQ 639
++EK++RL+ E E+K KDA K ++ +K E A+++ K ++Q
Sbjct: 811 LKEKQERLKAKKKEEEEKELEKRSKTKDAKKYFESWKSKKDEELKEAHRAKMQELKKQKQ 870
Query: 640 LEEEKK 657
EEE+K
Sbjct: 871 KEEEEK 876
>SB_11898| Best HMM Match : DMP1 (HMM E-Value=1.6)
Length = 1705
Score = 30.3 bits (65), Expect = 1.7
Identities = 18/56 (32%), Positives = 29/56 (51%)
Frame = +1
Query: 502 AEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISLS 669
A KK + ++ +D ++TG QK+S+ + A E+ K QL E +K LS
Sbjct: 1428 AGKKCDSQVRYDEDLNETGRKKHKQKRSKIIDIDFANSEKEKVVTQLSESRKRKLS 1483
>SB_30472| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 482
Score = 29.9 bits (64), Expect = 2.2
Identities = 21/74 (28%), Positives = 36/74 (48%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 654
+EK + + E KKR M + ++ K +QK+ E L N LE + + E+E+
Sbjct: 119 KEKAEEVNERAKKRALMYKYHEEKLKEDKRLNLQKQQEE--LRNKILEDVQALQ--EDER 174
Query: 655 KISLSIRIKPLTIE 696
+L +R K +E
Sbjct: 175 NKTLQVRNKQREVE 188
>SB_49860| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 362
Score = 29.5 bits (63), Expect = 2.9
Identities = 18/61 (29%), Positives = 30/61 (49%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 654
E++R++ EE E+ Q Q + ++ Q+K E+ L L+R K EEE+
Sbjct: 218 EKRRKKKEEQERLEQQRRQREQRIAEKRKRLEEQRKVESLHLLKELLKRVADKRAEEEEE 277
Query: 655 K 657
K
Sbjct: 278 K 278
>SB_26233| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 463
Score = 29.5 bits (63), Expect = 2.9
Identities = 16/62 (25%), Positives = 32/62 (51%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 654
EE+ ++ EEAE+K+Q L+ + + Q+K E + + + KE+ E++
Sbjct: 277 EEEEKQKEEAERKKQEKLEKLAKKKEELKERKRQEKLEQKAKKKEKERQEREKEKEREKE 336
Query: 655 KI 660
+I
Sbjct: 337 RI 338
>SB_54309| Best HMM Match : SH2 (HMM E-Value=5.1e-17)
Length = 1249
Score = 29.5 bits (63), Expect = 2.9
Identities = 18/65 (27%), Positives = 32/65 (49%), Gaps = 4/65 (6%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQ----LERNKTKEQL 642
E++RQR E+ ++ + +Q +KD + I ++ E Q L+R K +
Sbjct: 440 EDERQRAEDERQRVKHEMQRLKDERRRAEEDEIARQEEELRRLQEQREKELKRYKELQMA 499
Query: 643 EEEKK 657
EEEK+
Sbjct: 500 EEEKR 504
>SB_13201| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 383
Score = 29.5 bits (63), Expect = 2.9
Identities = 20/73 (27%), Positives = 39/73 (53%)
Frame = +1
Query: 469 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 648
DI K++ EE +KKR+ + ++ + +KKS + +AQ+E + + QLE
Sbjct: 252 DIGIKQKNPEECQKKRRKRRNLVSPEAEVNAKRSQEKKSVSL---SAQIEPD-LRNQLER 307
Query: 649 EKKISLSIRIKPL 687
E++ L+ + + L
Sbjct: 308 ERRNDLNTKFQKL 320
>SB_46756| Best HMM Match : zf-C2H2 (HMM E-Value=4.60046e-42)
Length = 1078
Score = 29.1 bits (62), Expect = 3.9
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNA-QLERNKTKEQLEEE 651
EE+RQ+ EEA K+R+ L + T + ++K G S A + +K E +E
Sbjct: 537 EERRQKREEARKRREEKLAKKGPTTSTSSSRKRRRKFNRNGRSAAIKAAASKLSENEDET 596
Query: 652 KK 657
K
Sbjct: 597 TK 598
>SB_46344| Best HMM Match : ig (HMM E-Value=0.0082)
Length = 181
Score = 28.7 bits (61), Expect = 5.1
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -2
Query: 86 LGTGSWTGSTATLECGVRGXP 24
+ T SW G +L+C VRG P
Sbjct: 1 MNTSSWDGKVLSLQCKVRGLP 21
>SB_23345| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 903
Score = 28.7 bits (61), Expect = 5.1
Identities = 28/149 (18%), Positives = 58/149 (38%), Gaps = 1/149 (0%)
Frame = +1
Query: 214 KQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAXXXXXXXXXXXXQAKRKVSXXXX 393
++E + E KR++++R +E+ K+ E RKQR + +++
Sbjct: 449 EEEERREEEERKREEEERKQREEEEKKREEEERKQREEEERKQKEKEEEKKRKEEERKHK 508
Query: 394 XXXXXXXXXXXXXXXXXXXXXXXXXDIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTI 573
+ ++R+R E EKK + ++ K T+
Sbjct: 509 EEEEKKTEESYDDSWRGRRARRKAEEERKERERRERLEKKHSEPEKKTEEPPKVVVEPTV 568
Query: 574 QKKSENFGLSNAQLERNKTKEQ-LEEEKK 657
+K+ ++E KT+E+ L++E K
Sbjct: 569 EKEPA------VKVESEKTEEEPLDDEAK 591
>SB_49663| Best HMM Match : TolA (HMM E-Value=0.059)
Length = 591
Score = 28.7 bits (61), Expect = 5.1
Identities = 15/55 (27%), Positives = 30/55 (54%)
Frame = +1
Query: 511 KRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISLSIR 675
+++A +++K+ + TIQ+ E+F +LE K KEQ E+ S +++
Sbjct: 214 QQKAQQESLKETLQEQEKETIQQLEEDFKAQLNELEVEKAKEQTALEEMKSFTVK 268
>SB_42720| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2391
Score = 28.7 bits (61), Expect = 5.1
Identities = 15/28 (53%), Positives = 18/28 (64%), Gaps = 2/28 (7%)
Frame = +3
Query: 525 APGHE--RCQQDRTQLHHPKEERKLRFE 602
APGH+ RC QD +L+ K ERK R E
Sbjct: 1105 APGHQPLRCLQDVEELYLSKVERKDRLE 1132
>SB_15709| Best HMM Match : CAP_GLY (HMM E-Value=0)
Length = 729
Score = 28.7 bits (61), Expect = 5.1
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +1
Query: 469 DIEEKRQRLEEAEKKRQAMLQAMKD 543
D+EEK + LEEA+ + +A+ MKD
Sbjct: 522 DLEEKAKELEEAKSENEAISGKMKD 546
>SB_53860| Best HMM Match : fn3 (HMM E-Value=3.6e-07)
Length = 288
Score = 28.3 bits (60), Expect = 6.8
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 98 VTQSLGTGSWTGSTATLECGVRGXP 24
V+ + T SW G +L+C RG P
Sbjct: 2 VSSTTNTSSWDGKVLSLQCKARGVP 26
>SB_47823| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 671
Score = 28.3 bits (60), Expect = 6.8
Identities = 14/57 (24%), Positives = 29/57 (50%)
Frame = +1
Query: 475 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLE 645
EEKR EEA K+++++ + + + K T +K+ E ++ N+ ++ E
Sbjct: 459 EEKRTESEEAPKRKKSLKRRLSFSRKNKAKKTEEKQDEEGEAREGTMDDNEVSQKPE 515
>SB_37749| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1103
Score = 28.3 bits (60), Expect = 6.8
Identities = 15/56 (26%), Positives = 30/56 (53%)
Frame = +1
Query: 490 RLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKK 657
R + E++RQA + ++DA + I+++ E A LER + +E+ E ++
Sbjct: 574 RRQRTERQRQAKAREVRDAREKERQDRIRRERERIARERA-LERERERERERERER 628
>SB_30292| Best HMM Match : fn3 (HMM E-Value=1.2e-12)
Length = 519
Score = 28.3 bits (60), Expect = 6.8
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = -2
Query: 104 KSVTQSLGTGSWTGSTATLECGVRGXP 24
K ++ T SW G +L+C RG P
Sbjct: 368 KDGASTMNTSSWDGKVLSLQCKARGLP 394
>SB_2413| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 493
Score = 28.3 bits (60), Expect = 6.8
Identities = 12/40 (30%), Positives = 25/40 (62%)
Frame = +1
Query: 205 PAPKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQRA 324
PA ++EGEG+P+ +R+ Q + ++Q K + +K ++
Sbjct: 56 PANEEEGEGEPKPKRRKAQPSAPKEKQTKSRKRDKKKDKS 95
>SB_48387| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 941
Score = 28.3 bits (60), Expect = 6.8
Identities = 14/57 (24%), Positives = 31/57 (54%)
Frame = +1
Query: 496 EEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISL 666
EE +++Q Q + + K I +++ + +LE++ +K+Q+EE+K + L
Sbjct: 344 EEQAQRKQLEKQLVDSSKKFEKENQILMEADEL-IKTLKLEKSSSKQQIEEQKNVCL 399
>SB_47629| Best HMM Match : Vicilin_N (HMM E-Value=1.1)
Length = 599
Score = 28.3 bits (60), Expect = 6.8
Identities = 20/80 (25%), Positives = 38/80 (47%)
Frame = +1
Query: 478 EKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKK 657
++ +R +E EKK + + K+ K ++K E + + + KE+ + EKK
Sbjct: 30 KRDEREQEREKKERRKKERKKERKKERKK---ERKKEKKKERKKERKEERKKERKKAEKK 86
Query: 658 ISLSIRIKPLTIEGLSVDKL 717
+ S + EGL+V K+
Sbjct: 87 FTDSNESVLVWTEGLNVSKV 106
>SB_22747| Best HMM Match : 7tm_1 (HMM E-Value=3.1e-06)
Length = 337
Score = 27.9 bits (59), Expect = 9.0
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -3
Query: 406 RGAFLLRHEKPCAWPASL*GV 344
RGA L R+E PC W A L G+
Sbjct: 69 RGAALQRNEPPCMWIAILNGI 89
>SB_53672| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 675
Score = 27.9 bits (59), Expect = 9.0
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 559 PNFTIQKKSENFGLSNAQLERNKTKEQLEEE 651
P T + EN GLSN L ++KTK E+
Sbjct: 498 PTPTSSQTQENIGLSNGTLTQDKTKPGCSED 528
>SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0)
Length = 442
Score = 27.9 bits (59), Expect = 9.0
Identities = 16/68 (23%), Positives = 36/68 (52%)
Frame = +1
Query: 478 EKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKK 657
++++RLE +KK Q L+ + K +K+ E +LE+ + KE+ + +K+
Sbjct: 339 KEKERLEAKQKKEQERLEKQAEKEKKEKERLEKKQRE----EKDRLEKKEKKEEEKRKKE 394
Query: 658 ISLSIRIK 681
++ +I+
Sbjct: 395 EEINAKIE 402
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.314 0.130 0.362
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,652,406
Number of Sequences: 59808
Number of extensions: 234923
Number of successful extensions: 919
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 838
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 912
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1962001171
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
- SilkBase 1999-2023 -