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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_pT_M20
         (670 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_21821| Best HMM Match : MRG (HMM E-Value=0)                        203   1e-52
SB_10431| Best HMM Match : No HMM Matches (HMM E-Value=.)              58   5e-09
SB_27426| Best HMM Match : RseA_C (HMM E-Value=6.5)                    41   0.001
SB_48371| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.5  
SB_24078| Best HMM Match : cNMP_binding (HMM E-Value=1.4e-26)          29   2.6  
SB_44278| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   3.4  
SB_57099| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   3.4  
SB_30168| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.0  
SB_19285| Best HMM Match : Galactosyl_T (HMM E-Value=0.69)             28   6.0  
SB_56054| Best HMM Match : rve (HMM E-Value=0.011)                     28   7.9  
SB_12316| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.9  

>SB_21821| Best HMM Match : MRG (HMM E-Value=0)
          Length = 292

 Score =  203 bits (495), Expect = 1e-52
 Identities = 98/197 (49%), Positives = 138/197 (70%)
 Frame = -2

Query: 666 TQSKDTPADSGSDQPKKKRGRLDLSIESEEQYLAKVEVKIKIPEELKVWLVDDWDVITRQ 487
           + S+ T  +  +   ++KR RL+ +++++E Y+ +V+VKI IP++LK WLVDDW++ITRQ
Sbjct: 89  SDSEKTKLEEVAGPTRRKRTRLNNTLDNDENYVPRVDVKIVIPDDLKQWLVDDWELITRQ 148

Query: 486 QKLAILPAKLTVSQIVDNYLAFKKSSKSHNQAKESVLVDITEGIKEYFNATLGSQLLYKF 307
           ++L  LP K TV+ I+D Y   K  +K+ N  K  V+ ++ +GI+EYFN  LG+QLLYKF
Sbjct: 149 KQLVPLPRKKTVADILDEYA--KHRAKTSNGVKPGVVREVADGIQEYFNVMLGTQLLYKF 206

Query: 306 ERPQYSEILQEYPDTPMSQVYGAVHLLRLFAKMGPMLAYTALDEKSLHHVLSHIQDFLKY 127
           ERPQY EIL E  + PMSQVYGA HLLRLF ++G  L+Y+ LDEK +  ++ HIQD L Y
Sbjct: 207 ERPQYGEILVENSNLPMSQVYGAEHLLRLFVRLGSALSYSNLDEKGVKFIVHHIQDLLDY 266

Query: 126 MVTNRSTLFNLQDYGNA 76
           MV N   LF+  DY  A
Sbjct: 267 MVKNADNLFS-TDYETA 282


>SB_10431| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 269

 Score = 58.4 bits (135), Expect = 5e-09
 Identities = 47/156 (30%), Positives = 76/156 (48%), Gaps = 14/156 (8%)
 Frame = -2

Query: 666 TQSKDTPADSGSDQPKKKRGRLDLSIESEEQYLAKVEVKIKIPEELKVWLVDDWDVITRQ 487
           T +  TP D  +  P          I S +  L KVE  I+IP +LK+ L DD  +I R+
Sbjct: 11  TSTSTTPVDQETTTPTD-------DITSNQSDLPKVE--IEIPSQLKLKLEDDCYLIKRK 61

Query: 486 QKLAI--------------LPAKLTVSQIVDNYLAFKKSSKSHNQAKESVLVDITEGIKE 349
           +K+ +              LP    V  +++ Y +  K ++       +++ ++ +G++ 
Sbjct: 62  KKVILSPLAFFGSLLLLIKLPRHPCVYDVLEEYFSHCKLTQPEESC--NLIREVLDGLRI 119

Query: 348 YFNATLGSQLLYKFERPQYSEILQEYPDTPMSQVYG 241
           YF+ TL + LLY FER QY   +Q YPD P+ Q  G
Sbjct: 120 YFDFTLPTLLLYNFEREQYYAAMQVYPD-PLGQSNG 154


>SB_27426| Best HMM Match : RseA_C (HMM E-Value=6.5)
          Length = 154

 Score = 40.7 bits (91), Expect = 0.001
 Identities = 19/48 (39%), Positives = 31/48 (64%)
 Frame = -2

Query: 384 SVLVDITEGIKEYFNATLGSQLLYKFERPQYSEILQEYPDTPMSQVYG 241
           +++ ++ +G++ YF+ TL + LLY FER QY   +Q  PD P+ Q  G
Sbjct: 3   NLIREVLDGLRIYFDFTLPTLLLYNFEREQYYAAMQVNPD-PLGQSNG 49


>SB_48371| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 811

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 28/105 (26%), Positives = 49/105 (46%), Gaps = 2/105 (1%)
 Frame = -2

Query: 570 LAKVEVKIKIPEELKVWLVDD--WDVITRQQKLAILPAKLTVSQIVDNYLAFKKSSKSHN 397
           +  ++ K K  E+L+  L+D+  W  +     + I P+ LT  Q  +N            
Sbjct: 403 ITDIKEKGKFKEKLQQHLIDNGQWFYVF----VWIQPSTLTQIQTFENANVICMICACSE 458

Query: 396 QAKESVLVDITEGIKEYFNATLGSQLLYKFERPQYSEILQEYPDT 262
           Q +  V+V I  GI+    +TL +++LY F    +    Q+YPD+
Sbjct: 459 QLRYDVIVFIFIGIRP---STLQAKVLYGFILIHFRRRFQKYPDS 500


>SB_24078| Best HMM Match : cNMP_binding (HMM E-Value=1.4e-26)
          Length = 692

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 12/49 (24%), Positives = 28/49 (57%)
 Frame = -2

Query: 480 LAILPAKLTVSQIVDNYLAFKKSSKSHNQAKESVLVDITEGIKEYFNAT 334
           L++L  ++   Q V ++  +KK S+   + K +++ ++ +G   + NAT
Sbjct: 540 LSMLVREIQEEQAVLSHAQYKKDSRKTQERKNAIISELKKGYSVFHNAT 588


>SB_44278| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 565

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 9/18 (50%), Positives = 14/18 (77%)
 Frame = -2

Query: 558 EVKIKIPEELKVWLVDDW 505
           E++ +  EE+K WL+DDW
Sbjct: 371 EMRSRYDEEMKAWLLDDW 388


>SB_57099| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2509

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 23/89 (25%), Positives = 42/89 (47%), Gaps = 7/89 (7%)
 Frame = -2

Query: 606  RLDLSIESEEQYLAKVEVKIKIPEELKVWLVDDWDVITRQQKLA--ILPAKLTVSQI--- 442
            +LD  ++ E  +   V   ++  +E+K  L+D  D+I+  Q L+  + P K   S++   
Sbjct: 991  KLDTQVQLEPLFKDVVTDMLQFKQEMKQELIDYKDIISSLQLLSKQVTPMKQRKSKLRQK 1050

Query: 441  --VDNYLAFKKSSKSHNQAKESVLVDITE 361
              V    ++K    +  Q +E  L D TE
Sbjct: 1051 TSVKAVCSYKNMQNTVEQGEEYTLFDNTE 1079


>SB_30168| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 6863

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 17/74 (22%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
 Frame = -2

Query: 666 TQSKDTPADSGSDQPKKKRGRLDLSIESEEQYLAKVE-VKI---KIPEELKVWLVDDWDV 499
           T+     +     Q +K+  +    I+S E ++ +++ +K    ++PE+    LV  W++
Sbjct: 510 TEKSGDESQQDLQQKQKRLQKFRQEIQSREYHITRIKSIKYPQWQVPEDKLSVLVSQWEI 569

Query: 498 ITRQQKLAILPAKL 457
           + R  K+ +L  KL
Sbjct: 570 VRR--KVTVLEEKL 581


>SB_19285| Best HMM Match : Galactosyl_T (HMM E-Value=0.69)
          Length = 263

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 21/71 (29%), Positives = 33/71 (46%)
 Frame = -2

Query: 474 ILPAKLTVSQIVDNYLAFKKSSKSHNQAKESVLVDITEGIKEYFNATLGSQLLYKFERPQ 295
           I P+ LT  Q  +N            Q +  V+V I  GI+    +TL +++LY F    
Sbjct: 6   IQPSTLTQIQTFENANVICMICACSEQLRYDVIVFIFIGIRP---STLQAKVLYGFILIH 62

Query: 294 YSEILQEYPDT 262
           +    Q+YPD+
Sbjct: 63  FRRRFQKYPDS 73


>SB_56054| Best HMM Match : rve (HMM E-Value=0.011)
          Length = 372

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 15/49 (30%), Positives = 22/49 (44%)
 Frame = -2

Query: 666 TQSKDTPADSGSDQPKKKRGRLDLSIESEEQYLAKVEVKIKIPEELKVW 520
           T +K T   + + + KKKR R   S     +     E   +IP EL+ W
Sbjct: 244 TNAKPTDTTAATSKNKKKRNRRKKSKSKTSEATENPEGASEIPPELQEW 292


>SB_12316| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 676

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 2/48 (4%)
 Frame = -2

Query: 408 KSHNQAKESVLVDITEG--IKEYFNATLGSQLLYKFERPQYSEILQEY 271
           K  +  KES   ++ +G   K Y N   G   +YK +R + +E+  EY
Sbjct: 378 KKKDLLKESEGAEVLKGDEFKRYVNKLRGKSTVYKKKRQELAELRAEY 425


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,491,977
Number of Sequences: 59808
Number of extensions: 300273
Number of successful extensions: 1077
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 947
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1067
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1729817375
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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