Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_M16
(734 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 298 6e-82
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 251 7e-68
SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3... 32 0.098
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 28 1.6
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 26 4.8
SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo... 26 6.4
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 26 6.4
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 8.5
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 8.5
SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomy... 25 8.5
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 8.5
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 298 bits (731), Expect = 6e-82
Identities = 135/238 (56%), Positives = 165/238 (69%)
Frame = -2
Query: 733 CKGQELYHFMGCSTFSQYTVVXEISLCKVAEAAPLDKVCLLGCGVPTGYGAALNTAKVEP 554
C G L HFMGCSTFS+YTVV +IS+ + APLD VCLLGCG+ TGYGAA TA ++
Sbjct: 137 CNGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLGCGITTGYGAATITADIKE 196
Query: 553 GSNCAIFXXXXXXXXXXXXXXXXXANRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQ 374
G + A+F A RI G+D+NP+K A FG +F+NP D PIQ
Sbjct: 197 GDSVAVFGLGSVGLAVIQGAVKKRAGRIFGIDVNPEKKNWAMSFGATDFINPNDLQSPIQ 256
Query: 373 QVLVDLTDGGLEYTFECIGNVGTMRAALEACHKGWGVSVIIGVAAAGEEISTRPFQLVTG 194
VL+ TDGGL++TF+C GNV MR+ALEACHKGWG S++IGVAAAG+EISTRPFQLVTG
Sbjct: 257 DVLIHETDGGLDWTFDCTGNVHVMRSALEACHKGWGQSIVIGVAAAGQEISTRPFQLVTG 316
Query: 193 RTWKGTAFGGYKSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEINEAFHLMHAGKAXR 20
R W+G AFGG K R +P LV EYL+ KL +D+++TH PLKEINEAF MH G +
Sbjct: 317 RVWRGCAFGGVKGRSQLPDLVKEYLDHKLEIDKYITHRRPLKEINEAFTDMHNGNCIK 374
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 251 bits (615), Expect = 7e-68
Identities = 118/243 (48%), Positives = 158/243 (65%)
Frame = -2
Query: 733 CKGQELYHFMGCSTFSQYTVVXEISLCKVAEAAPLDKVCLLGCGVPTGYGAALNTAKVEP 554
C+ + L H+MGCS+FSQYTVV +ISL ++ +APL +CLLGCGV TG+GA ++AKVE
Sbjct: 134 CRDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSICLLGCGVTTGFGAVTHSAKVES 193
Query: 553 GSNCAIFXXXXXXXXXXXXXXXXXANRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQ 374
GS A+ A+RII +DIN DK AKKFG +F++ K +
Sbjct: 194 GSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDINADKEVYAKKFGATDFIDSSKV-KDLV 252
Query: 373 QVLVDLTDGGLEYTFECIGNVGTMRAALEACHKGWGVSVIIGVAAAGEEISTRPFQLVTG 194
Q ++D+TDGG++Y F+C GNV M+ L+ CHKGWG +IGVAAAG+ + RPF +VTG
Sbjct: 253 QYVIDVTDGGVDYAFDCTGNVTVMQQELQFCHKGWGKLCVIGVAAAGKTLDFRPFLVVTG 312
Query: 193 RTWKGTAFGGYKSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEINEAFHLMHAGKAXRAG 14
R G+AFGG K R +P VDEY++ +DE++T+ PLK IN+AF MH GK R
Sbjct: 313 RQVLGSAFGGVKGRSELPNFVDEYMQGHFKVDEYITNEEPLKNINKAFDHMHEGKCIRCV 372
Query: 13 VDM 5
VDM
Sbjct: 373 VDM 375
>SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 260
Score = 31.9 bits (69), Expect = 0.098
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -2
Query: 475 RIIGVDINPDKFEVAKKFGVNEFVNP 398
R++G+D +PD + A++ G+N +V P
Sbjct: 57 RVVGIDASPDMIKAARELGLNAYVIP 82
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 174 AVPFQVRPVTSWNGRVLISSPAAATPMIT 260
+VP+ PVTS N + S+P +TP+ T
Sbjct: 501 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 529
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 174 AVPFQVRPVTSWNGRVLISSPAAATPMIT 260
+VP+ PVTS N + S+P +TP+ T
Sbjct: 560 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 588
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 26.2 bits (55), Expect = 4.8
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -2
Query: 658 LCKVAEAAPLDKVCLLGCGVPTGY 587
+CKV E A L CLL C T +
Sbjct: 979 ICKVREIASLSLTCLLDCSKMTTF 1002
>SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter
Itr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 575
Score = 25.8 bits (54), Expect = 6.4
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = -2
Query: 268 GVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGG 164
G V+IG + G E++ + +T T G GG
Sbjct: 110 GALVVIGTSLGGHELTNGGKEFITSATSLGALLGG 144
>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 489
Score = 25.8 bits (54), Expect = 6.4
Identities = 11/31 (35%), Positives = 14/31 (45%), Gaps = 3/31 (9%)
Frame = -1
Query: 248 CSCCW---RRDQHSSIPTCYRSHLEGNSFWR 165
C CW + D + P C R + E N WR
Sbjct: 42 CRFCWHHIKEDLNGRCPACRRLYTEENVQWR 72
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.4 bits (53), Expect = 8.5
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = -2
Query: 379 IQQVLVDLTDGGLEYTFECIGNVGTMRAALEACH 278
I Q + DLT G + +C+ + A+L CH
Sbjct: 3280 IVQFITDLTVGEFQLCLKCLLSFSQHAASLRICH 3313
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 25.4 bits (53), Expect = 8.5
Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +3
Query: 219 VLISSPA-AATPMITDTPHPLWQASSAALMVPTFPIHSKVYSRPPSVK 359
VL++S A A T + H A +A +++P+F + SK+ + S+K
Sbjct: 1000 VLVNSEADTALRAKTTSQHLKQSAMAAKVVLPSFIVTSKISKQTKSIK 1047
>SPAC20G8.03 |itr2||MFS myo-inositol transporter|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 557
Score = 25.4 bits (53), Expect = 8.5
Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -2
Query: 307 TMRAALEACHKGWGVSVIIGVA-AAGEEIS 221
++ AA E H+GW + IG A A G+ IS
Sbjct: 227 SLNAAFEHVHQGWRIMFGIGAAPALGQLIS 256
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 8.5
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +1
Query: 112 SSPSTHLQALVH-FLYFCNLQKLFPSRCD 195
SSPS+ L+ F Y CN+ KL+ + D
Sbjct: 914 SSPSSDLEVKQQAFTYVCNISKLYVYKSD 942
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,882,994
Number of Sequences: 5004
Number of extensions: 56785
Number of successful extensions: 180
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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